Mechanical ventilation modulates Toll-like receptor signaling pathway in a sepsis-induced lung injury model
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- Villar, J., Cabrera, N., Casula, M. et al. Intensive Care Med (2010) 36: 1049. doi:10.1007/s00134-010-1799-3
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Experimental and clinical studies on sepsis have demonstrated activation of the innate immune response following the initial host–bacterial interaction. In addition, mechanical ventilation (MV) can induce a pulmonary inflammatory response. How these two responses interact when present simultaneously remains to be elucidated. We hypothesized that MV modulates innate host response during sepsis by influencing Toll-like receptor (TLR) signaling.
Prospective, randomized, controlled animal study.
Male, septic Sprague–Dawley rats.
Sepsis was induced by cecal ligation and perforation. At 18 h, surviving animals had the cecum removed and were randomized to spontaneous breathing or two strategies of MV for 4 h: high (20 ml/kg) tidal volume (VT) with no positive end-expiratory pressure (PEEP) versus low VT (6 ml/kg) plus 10 cmH2O PEEP.
Measurements and main results
Histological evaluation, TLR-2, TLR-4, inhibitory kappaB alpha (IκBα), interleukin-1 receptor-associated kinase-3 (IRAK-3) gene expression, protein levels and immunohistochemical lung localization, inflammatory cytokines gene expression, and protein serum concentrations were analyzed. MV with low VT plus PEEP attenuated sepsis-associated TLR-4 activation, and produced a significant decrease of IRAK-3 gene expression and protein levels, a significant increase of IκBα, and a decrease in lung gene expression and serum levels of cytokines. High-VT MV caused a significant increase of TLR-4 and IRAK-3 protein levels, lung and systemic cytokines, and mortality, and a significant decrease of IκBα.
Our findings suggest a novel mechanism that could partially explain how MV modulates the innate immune response in the lung by interfering with cellular signaling pathways that are activated in response to pathogens.