Intensive Care Medicine

, Volume 33, Issue 4, pp 694–702

Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping

  • Jochen Kick
  • Balázs Hauser
  • Hendrik Bracht
  • Maura Albicini
  • Sükrü Öter
  • Florian Simon
  • Ulrich Ehrmann
  • Catherine Garrel
  • Jörn Sträter
  • Uwe B. Brückner
  • Xavier M. Leverve
  • Hubert Schelzig
  • Günter Speit
  • Peter Radermacher
  • Claus-Martin Muth
Experimental

DOI: 10.1007/s00134-006-0518-6

Cite this article as:
Kick, J., Hauser, B., Bracht, H. et al. Intensive Care Med (2007) 33: 694. doi:10.1007/s00134-006-0518-6

Abstract

Objective

We previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing SOD, catalase and residual glutathione peroxidase (GPx), protected against DNA strand-break damage induced by hyperbaric oxygen (HBO), a well-established model of DNA damage resulting from oxidative stress. Aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether this melon extract/gliadin would also reduce DNA damage after aortic cross-clamping and reperfusion.

Design

Prospective, randomized, controlled experimental study.

Setting

Animal laboratory.

Patients and participants

18 anesthetized, mechanically ventilated and instrumented pigs.

Interventions

After 14 days of oral administration of 1250 mg of the melon extract/gliadin (n = 9) or vehicle (n = 9), animals underwent 30 min of thoracic aortic cross-clamping and 4 h of reperfusion.

Measurements and results

Before clamping, immediately before declamping, and at 2 and 4 h of reperfusion, we measured blood isoprostane (immunoassay) and malondialdehyde concentrations (fluorimetric thiobarbituric acid test), SOD, catalase and GPx activities (spectrophotometric kits), NO formation (nitrate+nitrite; chemoluminescence), DNA damage in whole blood samples and isolated lymphocytes exposed to hyperbaric oxygen (comet assay). Organ function was also evaluated. Kidney and spinal cord specimen were analysed for apoptosis (TUNEL assay). The melon extract/gliadin blunted the DNA damage, reduced spinal cord apoptosis and attenuated NO release, however, without any effect on lipid peroxidation and organ function.

Conclusions

Pre-treatment with the oral melon extract/gliadin may be a therapeutic option to reduce oxidative cell injury affiliated with aortic cross-clamping.

Keywords

Ischemia/reperfusion injuryHyperbaric oxygenComet assayApoptosisNitric oxideKidneySpinal cord

Supplementary material

134_2006_518_MOESM1_ESM.doc (94 kb)
Electronic Supplementary Material (DOC 94K)

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jochen Kick
    • 1
    • 2
  • Balázs Hauser
    • 2
    • 8
  • Hendrik Bracht
    • 2
  • Maura Albicini
    • 2
    • 9
  • Sükrü Öter
    • 2
    • 10
  • Florian Simon
    • 1
    • 2
  • Ulrich Ehrmann
    • 2
  • Catherine Garrel
    • 3
  • Jörn Sträter
    • 4
  • Uwe B. Brückner
    • 5
  • Xavier M. Leverve
    • 6
  • Hubert Schelzig
    • 1
  • Günter Speit
    • 7
  • Peter Radermacher
    • 2
  • Claus-Martin Muth
    • 2
  1. 1.Abteilung Thorax- und GefässchirurgieUniversitätsklinikum UlmUlmGermany
  2. 2.Sektion Anästhesiologische Pathophysiologie und VerfahrensentwicklungUniversitätsklinikum UlmUlmGermany
  3. 3.Département de Biologie Intégrée, Centre Hospitalier UniversitaireUniversité Joseph FourierGrenobleFrance
  4. 4.Abteilung PathologieUniversitätsklinikum UlmUlmGermany
  5. 5.Sektion Chirurgische ForschungUniversitätsklinikum UlmUlmGermany
  6. 6.Laboratoire de Bioénergétique Fondamentale et AppliquéeUniversité Joseph FourierGrenobleFrance
  7. 7.Abteilung HumangenetikUniversitätsklinikum UlmUlmGermany
  8. 8.Semmelweis EgyetemAneszteziológiai és Intenzív Terápiás KlinikaBudapestHungary
  9. 9.Istituto di Anestesiologia e Rianimazione dell’Universita degli Studi di Milano, Azienda OspedalieraPolo Universitario San PaoloMilanoItaly
  10. 10.Fizyoloji Anabilim DaliGülhane Askeri Tip AkademisiAnkaraTurkey