Intensive Care Medicine

, Volume 28, Issue 7, pp 963–968

The effect of dehydroepiandrosterone on hemorrhage-induced suppression of cellular immune function

  • Reiner Oberbeck
  • Eike Nickel
  • Marthijn von Griensven
  • Thomas Tschernig
  • Tobias Wittwer
  • Daniel Schmitz
  • Hans-Christoph Pape
Experimental

DOI: 10.1007/s00134-002-1292-8

Cite this article as:
Oberbeck, R., Nickel, E., von Griensven, M. et al. Intensive Care Med (2002) 28: 963. doi:10.1007/s00134-002-1292-8

Abstract

Objective. To determine whether the steroid hormone dehydroepiandrosterone (DHEA) improves cellular immune functions after hemorrhagic shock.

Design and setting. Prospective controlled study in a research laboratory at an university medical center.

Subjects. Male NMRI mice.

Interventions. Animals received 0.9% saline or DHEA (20 mg/kg subcutaneously) before induction of a volume-controlled hemorrhagic shock (55% of estimated circulating blood volume) by retro-orbital puncture. One hour after hemorrhage mice underwent fluid resuscitation by intravenous infusion of lactated Ringer's solution (300% of the shed blood). Separate groups of mice were killed to obtain whole blood and spleen 1 h after hemorrhage, 1 h after fluid resuscitation, and 24 h after hemorrhage to determine lymphocyte distribution (CD4+, CD8+, NK1.1-AG+), splenocyte apoptosis, and plasma concentrations of tumor necrosis factor-α and interleukin-10.

Measurements and results. Hemorrhage in control mice was associated with a rapid increase in circulating NK cell numbers. Elevated splenocyte apoptosis, an increased CD4/CD8 ratio, and decreased number of circulating CD8+ T-cells was observed 24 h after hemorrhagic shock. DHEA administration was accompanied by a normalization of splenocyte apoptosis and lymphocyte migration. Induction of hemorrhagic shock did not affect TNF-α or IL-10 plasma concentrations in either treatment group.

Conclusions. DHEA administration improves cellular immune function after hemorrhage and may therefore be beneficial in patients with hemorrhagic shock.

Dehydroepiandrosterone Hemorrhage Shock Immune system Immune-endocrine interaction Mouse

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Reiner Oberbeck
    • 1
  • Eike Nickel
    • 2
  • Marthijn von Griensven
    • 2
  • Thomas Tschernig
    • 3
  • Tobias Wittwer
    • 2
  • Daniel Schmitz
    • 1
  • Hans-Christoph Pape
    • 2
  1. 1.Department of Trauma Surgery, University Hospital of Essen, Hufelandstrasse 55, 45122 Essen, GermanyGermany
  2. 2.Department of Trauma Surgery, Hannover Medical School, Hannover, GermanyGermany
  3. 3.Institute of Anatomy, Hannover Medical School, Hannover, GermanyGermany