Bulletin of Environmental Contamination and Toxicology

, Volume 90, Issue 4, pp 401–404

Ecotoxicity Bioassays on Leachates from Poultry Manure


    • Environmental DepartmentINIA
  • R. Miralles de Imperial
    • Environmental DepartmentINIA
  • F. Alonso
    • Agricultural and Livestock Facilities and Environment. EUIT AgrícolaUniversidad Politécnica de Madrid
  • C. Rodríguez
    • Environmental DepartmentINIA
  • J. V. Martín
    • Environmental DepartmentINIA

DOI: 10.1007/s00128-012-0936-9

Cite this article as:
Delgado, M., de Imperial, R.M., Alonso, F. et al. Bull Environ Contam Toxicol (2013) 90: 401. doi:10.1007/s00128-012-0936-9


The main aim of this study was to evaluate the toxicity of different poultry manure landfill leachates, using a well-known toxicity test system (MS3). The bioassay was made using a battery of toxicity tests including acute toxicity with crustacean (Daphnia magna), algae (Chlorella vulgaris) and the in vitro toxicity test with the fish cell line RTG-2. On D. magna was high mortality for zero time and almost 100 % and 70 %–80 % mortality for sawdust and straw poultry manure respectively. No effects on C. vulgaris, was observed after the leachate exposure. None of the parameters considered: protein, EROD activity, β-gal activity and neutral red, showed differences between control test and the leachate collected from exposure to poultry manure.


Poultry manureDaphnia magnaChlorella vulgarisIn vitro RTG-21

Copyright information

© Springer Science+Business Media New York 2012