Effects of Glyphosate and 2,4-D on Earthworms (Eisenia foetida) in Laboratory Tests


DOI: 10.1007/s00128-010-0089-7

Cite this article as:
Correia, F.V. & Moreira, J.C. Bull Environ Contam Toxicol (2010) 85: 264. doi:10.1007/s00128-010-0089-7


Laboratory tests were conducted to compare the effects of various concentrations of glyphosate and 2,4-D on earthworms (Eisenia foetida) cultured in Argissol during 56 days of incubation. The effects on earthworm growth, survival, and reproduction rates were verified for different exposure times. Earthworms kept in glyphosate-treated soil were classified as alive in all evaluations, but showed gradual and significant reduction in mean weight (50%) at all test concentrations. For 2,4-D, 100% mortality was observed in soil treated with 500 and 1,000 mg/kg. At 14 days, 30%–40% mortality levels were observed in all other concentrations. No cocoons or juveniles were found in soil treated with either herbicide. Glyphosate and 2,4-D demonstrated severe effects on the development and reproduction of Eisenia foetida in laboratory tests in the range of test concentrations.


Terrestrial ecotoxicology Earthworm Eisenia foetida Contaminated soils Herbicides 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Ecotoxicology LaboratoryCenter for Studies on Workers’ Health and Human EcologyManguinhosBrazil