Diabetologia

, Volume 44, Issue 10, pp 1274–1280

Nephrin is expressed in the pancreatic beta cells

Authors

  • T. Palmén
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
  • H. Ahola
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
  • J. Palgi
    • Haartman Institute, Transplantation Laboratory and Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
  • P. Aaltonen
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
  • P. Luimula
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
  • S. Wang
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
  • I. Jaakkola
    • National Public Health Institute, Medicity Research Laboratory, University of Turku, Turku, Finland
  • M. Knip
    • Haartman Institute, Transplantation Laboratory and Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
  • T. Otonkoski
    • Haartman Institute, Transplantation Laboratory and Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
  • H. Holthöfer
    • Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, and Helsinki University Central Hospital Helsinki, Finland
Article

DOI: 10.1007/s001250100641

Cite this article as:
Palmén, T., Ahola, H., Palgi, J. et al. Diabetologia (2001) 44: 1274. doi:10.1007/s001250100641

Abstract.

Aims/hypothesis:

The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types.

Methods:

RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas.

Results:

Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165 000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19.

Conclusion/interpretation:

These data show that nephrin is a novel molecule of pancreatic beta cells. [Diabetologia (2001) 44: 1274–1280]

KeywordsNPHS1 gene nephrin immunoglobulin superfamily islets of Langerhans beta cell.

Copyright information

© Springer-Verlag Berlin Heidelberg 2001