Diabetologia

, Volume 44, Issue 9, pp 1189–1196

The LEW.1AR1/Ztm-iddm rat: a new model of spontaneous insulin-dependent diabetes mellitus

Authors

  • S. Lenzen
    • Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
  • M. Tiedge
    • Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
  • M. Elsner
    • Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
  • S. Lortz
    • Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
  • H. Weiss
    • Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
  • A. Jörns
    • Centre of Anatomy, Hannover Medical School, Hannover, Germany
  • G. Klöppel
    • Institute of Pathology, University of Kiel, Kiel, Germany
  • D. Wedekind
    • Institute for Laboratory Animal Science, Hannover Medical School, Hannover, Germany
  • C. -M. Prokop
    • Institute for Laboratory Animal Science, Hannover Medical School, Hannover, Germany
  • H. J. Hedrich
    • Institute for Laboratory Animal Science, Hannover Medical School, Hannover, Germany

DOI: 10.1007/s001250100625

Cite this article as:
Lenzen, S., Tiedge, M., Elsner, M. et al. Diabetologia (2001) 44: 1189. doi:10.1007/s001250100625

Abstract.

Aims/hypothesis:

We describe a new Type I (insulin-dependent) diabetes mellitus rat model (LEW.1AR1/Ztm-iddm) which arose through a spontaneous mutation in a congenic Lewis rat strain with a defined MHC haplotype (RT1.A a B/D u C u ).

Methods:

The development of diabetes was characterised using biochemical, immunological and morphological methods.

Results:

Diabetes appeared in the rats with an incidence of 20 % without major sex preference at 58 ± 2 days. The disease was characterised by hyperglycaemia, glycosuria, ketonuria and polyuria. In peripheral blood, the proportion of T lymphocytes was in the normal range expressing the RT6.1 differentiation antigen. Islets were heavily infiltrated with B and T lymphocytes, macrophages and NK cells with beta cells rapidly destroyed through apoptosis in areas of insulitis.

Conclusion/interpretation:

This Type I diabetic rat develops a spontaneous insulin-dependent autoimmune diabetes through beta cell apoptosis. It could prove to be a valuable new animal model for clarifying the mechanisms involved in the development of autoimmune diabetes. [Diabetologia (2001) 44: 1189–1196]

Keywords Type I diabetes mellitus, animal model, rat, apoptosis.

Copyright information

© Springer-Verlag Berlin Heidelberg 2001