Diabetologia

, Volume 43, Issue 9, pp 1165–1169

Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells

  • M. Dubois
  • F. Pattou
  • J. Kerr-Conte
  • V. Gmyr
  • B. Vandewalle
  • P. Desreumaux
  • J. Auwerx
  • K. Schoonjans
  • J. Lefebvre
Short communication

DOI: 10.1007/s001250051508

Cite this article as:
Dubois, M., Pattou, F., Kerr-Conte, J. et al. Diabetologia (2000) 43: 1165. doi:10.1007/s001250051508

Abstract

Aims/hypothesis. Thiazolidinediones are reported to improve pancreatic islet morphology and beta-cell function in rodents, supporting the hypothesis of a direct action of thiazolidinediones on endocrine islet cells. In this study we examined the expression of the peroxisome proliferator-activated receptor γ, a nuclear receptor that is activated by naturally occurring fatty acids and synthetic thiazolidinediones, in normal human endocrine pancreatic cells. Methods. Human islets were isolated from pancreata harvested in ten brain-dead lean non-diabetic adult donors. We analysed the gene and protein expression of the human peroxisome proliferator-activated receptor γ and evaluated the effects of peroxisome proliferator-activated receptor γ agonist on insulin secretion in human islet preparations. Results. The RT-PCR carried out on total RNA from four distinct human islet preparations demonstrated the presence of peroxisome proliferator-activated receptor γ mRNA. Western blot analysis showed the consistent expression of peroxisome proliferator-activated receptor γ protein. Peroxisome proliferator-activated receptor γ was shown to be present in all three endocrine cell types studied (alpha, beta and delta cells) by immunohistochemistry. Conclusion/interpretation. We found that peroxisome proliferator-activated receptor γ is highly expressed in human islet endocrine cells, both at the mRNA and protein levels. These results support the hypothesis of a direct influence of peroxisome proliferator-activated receptor γ agonist on human pancreatic endocrine cells. [Diabetologia (2000) 43: 1165–1169]

Keywords Type II diabetesperoxisome proliferator-activated receptor γhuman pancreasisletsbeta cellthiazolidinedionestroglitazonerosiglitazone.
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© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Dubois
    • 1
  • F. Pattou
    • 1
  • J. Kerr-Conte
    • 1
  • V. Gmyr
    • 1
  • B. Vandewalle
    • 1
  • P. Desreumaux
    • 2
  • J. Auwerx
    • 3
  • K. Schoonjans
    • 3
  • J. Lefebvre
    • 1
  1. 1.UPRES 1048, University Hospital Centre of Lille, Lille, FranceFR
  2. 2.Research Laboratory of Inflammatory Intestinal Disorders (CRI4U004B), University Hospital Centre of Lille, Lille, FranceFR
  3. 3.Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, FranceFR