Diabetologia

, Volume 42, Issue 1, pp 113–116

Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans: a 1H NMR spectroscopy study

  • M. Krssak
  • K. Falk Petersen
  • A. Dresner
  • L. DiPietro
  • S. M. Vogel
  • D. L. Rothman
  • G. I. Shulman
  • M. Roden
Rapid communication

DOI: 10.1007/s001250051123

Cite this article as:
Krssak, M., Falk Petersen, K., Dresner, A. et al. Diabetologia (1999) 42: 113. doi:10.1007/s001250051123

Summary

Recent muscle biopsy studies have shown a relation between intramuscular lipid content and insulin resistance. The aim of this study was to test this relation in humans by using a novel proton nuclear magnetic resonance (1H NMR) spectroscopy technique, which enables non-invasive and rapid ( ∼ 45 min) determination of intramyocellular lipid (IMCL) content. Normal weight non-diabetic adults (n = 23, age 29 ± 2 years, BMI = 24.1 ± 0.5 kg/m2) were studied using cross-sectional analysis. Insulin sensitivity was assessed by a 2-h hyperinsulinaemic ( ∼ 450 pmol/l)-euglycaemic ( ∼ 5 mmol/l) clamp test. Intramyocellular lipid concentrations were determined by using localized 1H NMR spectroscopy of soleus muscle. Simple linear regression analysis showed an inverse correlation (r = –0.692, p = 0.0017) between intramyocellular lipid content and M-value (100–120 min of clamp) as well as between fasting plasma non-esterified fatty acid concentration and M-value (r = –0.54, p= 0.0267). Intramyocellular lipid content was not related to BMI, age and fasting plasma concentrations of triglycerides, non-esterified fatty acids, glucose or insulin. These results show that intramyocellular lipid concentration, as assessed non invasively by localized 1H NMR spectroscopy, is a good indicator of whole body insulin sensitivity in non-diabetic, non-obese humans. [Diabetologia (1999) 42: 113–116]

Keywords Intramyocellular lipids insulin sensitivity NMR spectroscopy skeletal muscle triglycerides. 
Download to read the full article text

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. Krssak
    • 1
  • K. Falk Petersen
    • 1
  • A. Dresner
    • 1
  • L. DiPietro
    • 2
  • S. M. Vogel
    • 1
  • D. L. Rothman
    • 1
  • G. I. Shulman
    • 3
  • M. Roden
    • 4
  1. 1.Department of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USAUS
  2. 2.The John B. Pierce Laboratory and Department of Epidemiology & Public Health Yale University School of Medicine, New Haven, Connecticut, USAUS
  3. 3.Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, USAUS
  4. 4.Division of Endocrinology and Metabolism, Department of Internal Medicine III, University of Vienna, Vienna, AustriaAT

Personalised recommendations