Rapid communication

Diabetologia

, Volume 42, Issue 1, pp 113-116

First online:

Intramyocellular lipid concentrations are correlated with insulin sensitivity in humans: a 1H NMR spectroscopy study

  • M. KrssakAffiliated withDepartment of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USA
  • , K. Falk PetersenAffiliated withDepartment of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USA
  • , A. DresnerAffiliated withDepartment of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USA
  • , L. DiPietroAffiliated withThe John B. Pierce Laboratory and Department of Epidemiology & Public Health Yale University School of Medicine, New Haven, Connecticut, USA
  • , S. M. VogelAffiliated withDepartment of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USA
  • , D. L. RothmanAffiliated withDepartment of Internal Medicine Yale University School of Medicine, New Haven, Connecticut, USA
  • , G. I. ShulmanAffiliated withHoward Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, USA
  • , M. RodenAffiliated withDivision of Endocrinology and Metabolism, Department of Internal Medicine III, University of Vienna, Vienna, Austria

Summary

Recent muscle biopsy studies have shown a relation between intramuscular lipid content and insulin resistance. The aim of this study was to test this relation in humans by using a novel proton nuclear magnetic resonance (1H NMR) spectroscopy technique, which enables non-invasive and rapid ( ∼ 45 min) determination of intramyocellular lipid (IMCL) content. Normal weight non-diabetic adults (n = 23, age 29 ± 2 years, BMI = 24.1 ± 0.5 kg/m2) were studied using cross-sectional analysis. Insulin sensitivity was assessed by a 2-h hyperinsulinaemic ( ∼ 450 pmol/l)-euglycaemic ( ∼ 5 mmol/l) clamp test. Intramyocellular lipid concentrations were determined by using localized 1H NMR spectroscopy of soleus muscle. Simple linear regression analysis showed an inverse correlation (r = –0.692, p = 0.0017) between intramyocellular lipid content and M-value (100–120 min of clamp) as well as between fasting plasma non-esterified fatty acid concentration and M-value (r = –0.54, p= 0.0267). Intramyocellular lipid content was not related to BMI, age and fasting plasma concentrations of triglycerides, non-esterified fatty acids, glucose or insulin. These results show that intramyocellular lipid concentration, as assessed non invasively by localized 1H NMR spectroscopy, is a good indicator of whole body insulin sensitivity in non-diabetic, non-obese humans. [Diabetologia (1999) 42: 113–116]

Keywords Intramyocellular lipids insulin sensitivity NMR spectroscopy skeletal muscle triglycerides.