Diabetologia

, Volume 41, Issue 8, pp 940–946

Uncoupling protein-2 gene: reduced mRNA expression in intraperitoneal adipose tissue of obese humans

  • H. Oberkofler
  • Y. M. Liu
  • H. Esterbauer
  • E. Hell
  • F. Krempler
  • W. Patsch
Originals

DOI: 10.1007/s001250051011

Cite this article as:
Oberkofler, H., Liu, Y., Esterbauer, H. et al. Diabetologia (1998) 41: 940. doi:10.1007/s001250051011

Summary

The mitochondrial uncoupling protein-2 (UCP-2) is a recently discovered homologue of the brown adipose tissue-specific uncoupling protein and could be involved in the regulation of energy balance. Since obesity is associated with disturbed energy homeostasis, we tested the hypothesis that UCP-2 gene expression is deficient in this disorder. We determined, by a competitive reverse transcription-polymerase chain reaction assay, UCP-2 mRNA expression in intra- and extraperitoneal adipose tissues of 107 morbidly obese subjects and 31 lean control subjects. In both obese and non-obese subjects, UCP-2 mRNA abundance was higher in the intraperitoneal than in the extraperitoneal tissue (p < 0.05), but no association was observed between intra- and extraperitoneal expression in either group. Compared with lean control subjects, both male and female obese subjects displayed significantly lower average UCP-2 mRNA expression in the intraperitoneal adipose tissue (p < 0.006), while UCP-2 mRNA abundance in extraperitoneal adipose tissue was not different between obese and non-obese men and women. Intraperitoneal UCP-2 mRNA remained low in nine obese subjects who lost 23 ± 12 kg of weight over a period of 10 ± 5 months subsequent to weight reducing surgery. These data support the concept that impaired adipose tissue expression of UCP-2 may play a role in the pathophysiology of obesity. [Diabetologia (1998) 41: 940–946]

Keywords Energy homeostasisgene expressionobesityRT-PCRuncoupling protein-2.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. Oberkofler
    • 1
  • Y. M. Liu
    • 1
  • H. Esterbauer
    • 1
  • E. Hell
    • 2
  • F. Krempler
    • 3
  • W. Patsch
    • 1
  1. 1.Department of Laboratory Medicine, Landeskrankenanstalten Salzburg, AustriaAT
  2. 2.Department of Surgery, Krankenhaus Hallein, AustriaAT
  3. 3.Department of Internal Medicine, Krankenhaus Hallein, AustriaAT