, Volume 56, Issue 8, pp 1705-1711
Date: 09 May 2013

Next-generation sequencing for viruses in children with rapid-onset type 1 diabetes

Abstract

Aims/hypothesis

Viruses are candidate causative agents in the pathogenesis of autoimmune (type 1) diabetes. We hypothesised that children with a rapid onset of type 1 diabetes may have been exposed to such agents shortly before the initiation of islet autoimmunity, possibly at high dose, and thus study of these children could help identify viruses involved in the development of autoimmune diabetes.

Methods

We used next-generation sequencing to search for viruses in plasma samples and examined the history of infection and fever in children enrolled in The Environmental Determinants of Diabetes in the Young (TEDDY) study who progressed to type 1 diabetes within 6 months from the appearance of islet autoimmunity, and in matched islet-autoantibody-negative controls.

Results

Viruses were not detected more frequently in plasma from rapid-onset patients than in controls during the period surrounding seroconversion. In addition, infection histories were found to be similar between children with rapid-onset diabetes and control children, although episodes of fever were reported less frequently in children with rapid-onset diabetes.

Conclusions/interpretation

These findings do not support the presence of viraemia around the time of seroconversion in young children with rapid-onset type 1 diabetes.

H.-S. Lee and T. Briese contributed equally to this study
Details of the TEDDY Study Group (co-authors) are provided as electronic supplementary material (ESM).