Diabetologia

, 54:2626

Synergistic induction of lipid catabolism and anti-inflammatory lipids in white fat of dietary obese mice in response to calorie restriction and n-3 fatty acids

  • P. Flachs
  • R. Rühl
  • M. Hensler
  • P. Janovska
  • P. Zouhar
  • V. Kus
  • Z. Macek Jilkova
  • E. Papp
  • O. Kuda
  • M. Svobodova
  • M. Rossmeisl
  • G. Tsenov
  • V. Mohamed-Ali
  • J. Kopecky
Article

DOI: 10.1007/s00125-011-2233-2

Cite this article as:
Flachs, P., Rühl, R., Hensler, M. et al. Diabetologia (2011) 54: 2626. doi:10.1007/s00125-011-2233-2

Abstract

Aims/hypothesis

Calorie restriction is an essential component in the treatment of obesity and associated diseases. Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) act as natural hypolipidaemics, reduce the risk of cardiovascular disease and could prevent the development of obesity and insulin resistance. We aimed to characterise the effectiveness and underlying mechanisms of the combination treatment with LC n-3 PUFA and 10% calorie restriction in the prevention of obesity and associated disorders in mice.

Methods

Male mice (C57BL/6J) were habituated to a corn-oil-based high-fat diet (cHF) for 2 weeks and then randomly assigned to various dietary treatments for 5 weeks or 15 weeks: (1) cHF, ad libitum; (2) cHF with LC n-3 PUFA concentrate replacing 15% (wt/wt) of dietary lipids (cHF + F), ad libitum; (3) cHF with calorie restriction (CR; cHF + CR); and (4) cHF + F + CR. Mice fed a chow diet were also studied.

Results

We show that white adipose tissue plays an active role in the amelioration of obesity and the improvement of glucose homeostasis by combining LC n-3 PUFA intake and calorie restriction in cHF-fed mice. Specifically in the epididymal fat in the abdomen, but not in other fat depots, synergistic induction of mitochondrial oxidative capacity and lipid catabolism was observed, resulting in increased oxidation of metabolic fuels in the absence of mitochondrial uncoupling, while low-grade inflammation was suppressed, reflecting changes in tissue levels of anti-inflammatory lipid mediators, namely 15-deoxy-Δ12,15-prostaglandin J2 and protectin D1.

Conclusions/interpretation

White adipose tissue metabolism linked to its inflammatory status in obesity could be modulated by combination treatment using calorie restriction and dietary LC n-3 PUFA to improve therapeutic strategies for metabolic syndrome.

Keywords

15-Deoxy-Δ12,15-prostaglandin J2DHAEPAFish oilMetabolic syndromeWhite adipose tissue

Abbreviations

15d-PGJ2

15-Deoxy-Δ12,15-prostaglandin J2

AA

Arachidonic acid

AMPK

AMP-activated protein kinase

cHF

Corn-oil-based high fat diet

cHF + F

cHF diet supplemented with LC n-3 PUFA concentrate (15% [wt/wt] of dietary lipids)

CLS

Crown-like structure

COX

Cyclooxygenase

CyOX

Cytochrome c oxidase

DHA

Docosahexaenoic acid

EPA

Eicosapentaenoic acid

FA

Fatty acid

FASTED

Fasted state, specifically defined

FCCP

Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone

LC n-3

Long-chain n-3

LOX

Lipoxygenase

NF-κB

Nuclear factor κ light-chain enhancer of activated B cells

MAC-2

β-Galactoside-binding lectin

PD1

Protectin D1

PGC-1α

PPARγ coactivator 1α

PLS-DA

Partial least squares-discriminant analysis

PPARγ

Peroxisome proliferator-activated receptor γ

PUFA

Polyunsaturated fatty acids

RE-FED

Re-fed state, specifically defined

SIRT1

Sirtuin 1 (silent mating type information regulation 2, homologue) 1

TG

Triacylglycerol

UCP1

Uncoupling protein 1

WAT

White adipose tissue

Introduction

Intrinsic metabolic properties and secretory functions of white adipose tissue (WAT) have a major impact on the development of chronic morbidities associated with obesity, including type 2 diabetes, dyslipidaemia and hypertension. When (post)prandial plasma levels of NEFA and triacylglycerol (TG) exceed the storage capacity of WAT [1], other tissues including the liver and muscle become overloaded with lipids, which results in insulin resistance, the key event in the pathophysiology of metabolic syndrome [2]. The important role of WAT in energy homeostasis is underscored by the findings that WAT is one of the key organs affected by calorie restriction, the most effective strategy to prolong a healthy life in several species [3], and by the fact that accumulation of body fat can be reduced through upregulation of lipid catabolism in WAT [410]. The metabolism and secretory functions of WAT are also modulated by long-chain (LC) n-3 polyunsaturated fatty acids (PUFA), namely eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (DHA; 22:6 n-3), which exert numerous beneficial effects on health, including improvements in lipid metabolism and prevention of obesity and diabetes [11], while decreasing the rate of fatal coronary heart disease in diabetic patients who have had a myocardial infarction [12]. The metabolic changes induced by both calorie restriction and LC n-3 PUFA include induction of mitochondrial biogenesis and lipid catabolism in WAT [3, 13].

Importantly, LC n-3 PUFA also decrease inflammation [14], including obesity-associated low-grade inflammation of WAT [15, 16], characterised by altered secretion patterns of adipokines, which contributes to insulin resistance [1]. The anti-inflammatory effects of LC n-3 PUFA probably depend on the formation of their active metabolites. These lipid mediators originate from either targeted enzymatic synthesis, such as resolvins and protectins [17, 18], or from non-enzymatic oxidation reactions [19, 20]. They can act as ligands for surface receptors or can interact with signalling proteins including the transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and nuclear factor κ light-chain enhancer of activated B cells (NF-κB) [19]. Notably, resolvins and protectins mediate the anti-inflammatory and protective actions of LC n-3 PUFA in obesity-induced insulin resistance and hepatic steatosis [17, 21]. As LC n-3 PUFA and LC n-6 PUFA compete for common enzymatic pathways, a relatively small increase in the LC n-3 PUFA intake usually slows down synthesis of pro-inflammatory metabolites derived from arachidonic acid (AA; 20:4 n-6) [17, 18]. Given the complexity of factors contributing to the development of metabolic syndrome, its prevention and treatment requires strategies combining several approaches. Clinical studies suggest [2224] that combining calorie restriction and LC n-3 PUFA intake may be helpful. As both treatments could promote fatty acid (FA) oxidation in WAT (see above), we have endeavoured in this study to establish whether lipid catabolism in WAT could be augmented by calorie restriction combined with LC n-3 PUFA intake. Our results in dietary obese mice show additive effects of calorie restriction and LC n-3 PUFA in the induction of mitochondrial biogenesis and lipid catabolism, occurring with a surprising specificity in intra-abdominal WAT, which could contribute to the beneficial systemic effects of the combination treatment. The tissue-specificity could be explained by formation of anti-inflammatory lipid mediators derived from both LC n-3 and LC n-6 PUFA.

Methods

Animal treatment

Male mice (C57BL/6J; Jackson Laboratory, ME, USA) were weaned onto standard laboratory chow (Chow; extruded R/M-H diet; Ssniff Spezialdiäten, Soest, Germany). Singly caged mice were habituated to a corn-oil-based high-fat diet (cHF; lipid content 35%, wt/wt; [16]) for 2 weeks starting at 2 months of age and then randomly assigned for 5 weeks (four independent experiments) or 15 weeks (one experiment) to various dietary treatments (see electronic supplementary material [ESM] Fig. 1): (1) cHF, ad libitum; (2) cHF supplemented with LC n-3 PUFA concentrate (46% wt/wt DHA, 14% wt/wt EPA; product EPAX 1050 TG; EPAX, Alesund, Norway) replacing 15% wt/wt of dietary lipids (cHF + F), ad libitum; (3) cHF with restriction of energy intake—the ration was reduced by 10% wt/wt compared with mice fed ad libitum with the same type of diet (CR; cHF + CR); and (4) cHF + F + CR. When indicated, plasma was collected by using tail bleeds during the FASTED to RE-FED transition (for the detailed definition of FASTED and RE-FED state, see ESM). An OGTT was performed in overnight-fasted mice as in Kuda et al. [16], except that glucose was administered by oral gavage. At the end of the treatment, mice were killed in a random fed state (between 08:00 hours and 10:00 hours).

Experiments were conducted according to the guidelines for the use and care of laboratory animals of the Institute of Physiology.

Tissue lipid content

The tissue content of TG was estimated in ethanolic KOH tissue solubilisates [25].

Energy expenditure and metabolic flexibility

Energy expenditure and fuel partitioning were evaluated using the indirect calorimetry system INCA (Somedic, Horby, Sweden) at 30°C [26]. Metabolic flexibility was assessed as a maximal induction in RQ (\( \dot{V}{\text{C}}{{\text{O}}_{{2}}}/\dot{V}{{\text{O}}_{{2}}} \)) values in response to a glucose load (0.45 ml of 50% [wt/vol.] d-glucose) administered by intragastric gavage to overnight (12 h) fasted animals. The induction was calculated from RQ values averaged over 60 min intervals before and after the gavage (ESM Fig. 2).

Physical activity

Animal behaviour was recorded by video camera and analysed off-line (see ESM).

Plasma variables

Levels of glucose, NEFA, TG, leptin, total adiponectin, IL-6, β-hydroxybutyrate and insulin were determined in plasma [16, 27].

Gene expression

Transcript levels were evaluated using quantitative real-time RT-PCR (see ESM).

Ex vivo biochemical analysis

FA oxidation was measured using [1-14C]palmitate in fragments of epididymal fat or gastrocnemius muscle or whole soleus muscle [28], in isolated adipocytes [29], and in hepatocytes isolated from mice following the in vivo treatment [30]. The rate of FA synthesis in epididymal fat was measured by incorporation of 3H2O into saponifiable FAs [9]. Mitochondrial respiration was evaluated using high-resolution respirometry in digitonin-permeabilised adipocytes [29] using OROBOROS Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria; see Fig. 4c,d legend).

Activity of cytochrome c oxidase and cytochrome b and protein content

Crude membrane fraction was used to evaluate activity of cytochrome c oxidase (CyOX) spectrophotometrically [10] and to quantify cytochrome b using a pseudo-dual-wavelength spectrophotometry [29].

Immunohistochemical analysis

Morphometry of adipocytes was performed (5 μm sections) using Lucia IMAGE software (Laboratory Imaging, Prague, Czech Republic). In the case of epididymal fat, sections were processed to detect β-galactoside-binding lectin (MAC-2) and perilipin [16].

Lipidomic analysis

In total, 24 lipid molecular species were quantified in epididymal fat and liver extracts using HPLC MS-MS analysis [31] (see ESM).

Statistical analysis

All values are presented as means ± SEM. Comparisons were judged to be significant at p ≤ 0.05 (see ESM).

Results

Combination treatment counteracts development of obesity and accumulation of ectopic fat

Feeding mice a cHF diet increased their body weight (Fig. 1a). Either cHF + F or cHF + CR treatment partially prevented the cHF-induced obesity, while the combination treatment (cHF + F + CR) provided full protection, as shown by the treatments lasting either 5 weeks (Fig. 1a and Table 1) or 15 weeks (see legend to Fig. 1). As already described [13, 30], food consumption was not affected by dietary LC n-3 PUFA (Table 1). Physical activity, evaluated after 3 weeks of the treatment (ESM Fig. 1) as total distance travelled and total duration of moving per 4 h, was similar in the cHF, cHF + F and cHF + CR mice, while it was lower in the cHF + F + CR mice (Table 1). Prevention of dietary obesity by various treatments correlated with reduction of both adiposity and fat cell size in all fat depots analysed at 5 weeks (Table 1). Significant differences in both variables were observed between the cHF mice and the cHF + F + CR mice, with the strongest effects elicited in the epididymal fat (Table 1). Feeding of the cHF diet induced accumulation of lipids in the liver and skeletal muscle. In the liver, all the treatments decreased TG accumulation and the effect of LC n-3 PUFA was stronger at 5 weeks (Fig. 1b) than at 15 weeks (Fig. 1c). In response to the longer treatment, the maximal reduction of TG accumulation in skeletal muscle was observed in the case of cHF + F + CR mice (Fig. 1e).
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Fig. 1

Prevention of diet-induced obesity, hepatic steatosis, and metabolic inflexibility. a Body weight of mice; week 0, start of the differential dietary treatment. Results from a typical experiment (n = 10; see also Table 1). White circles, cHF; black circles, cHF + F; white triangles, cHF + CR; black triangles, cHF + F + CR; grey circles, Chow. Five independent short-term (5 weeks) and one long-term (15 weeks) experiments were performed with similar results (not shown). After 15 weeks, body weights in mice fed different diets were as followed: cHF, 42.7 ± 1.2 g; cHF + F, 36.8 ± 1.9 g; cHF + CR, 34.7 ± 2.5 g; cHF + F + CR, 33.1 ± 0.8 g; p < 0.05 vs cHF for all the treatments. b–e Ectopic lipid accumulation in liver (b, c) and gastrocnemius muscle (d, e) after 5 weeks (b, d) or 15 weeks (c, e) of the treatment; n = 10. Data for the Chow-fed mice are shown only for week 5, but similar values at week 15 should be expected [16]. NEFA (f) and (g) glucose levels in plasma after 4 weeks of the treatment, measured in either the FASTED or RE-FED state (see ESM; n = 10). White circles, cHF; black circles, cHF + F; white triangles, cHF + CR; black triangles, cHF + F + CR; grey circles, Chow. h During the fourth week of the treatment, metabolic flexibility was assessed as a maximal induction in RQ values in response to a glucose load administered by intragastric gavage to animals fasted overnight (12 h) (n = 9–10). White bars, cHF or cHF + CR diet; black bars, cHF + F or cHF + F + CR. Data from an independent experiment using the Chow-fed mice showed ΔRQ = 0.15 ± 0.01 (n = 9). For details, see ESM Fig. 2 and ESM Table 1. Data are means ± SEM. a,b,cSignificant difference (ANOVA) compared with cHF, cHF + F, and cHF + CR, respectively; dsignificant difference (t test) compared with Chow-fed mice esignificant difference (repeated-measures ANOVA) between the FASTED and RE-FED states

Table 1

Body weight gain, food consumption, physical activity, organ weights, size of adipocytes and plasma variables in mice

Variable

cHF

cHF + F

cHF + CR

cHF + F + CR

Body weight gain (g)

3.7 ± 0.5

2.9 ± 0.6

2.1 ± 0.3a

0.8 ± 0.2a,b,c

Food consumption (kJ/day per animal)

74.6 ± 0.6

73.7 ± 1.8

67.1 ± 0.5a,b

66.3 ± 1.6a,b

Physical activity

  Total distance moved (m)

236 ± 22

231 ± 65

200 ± 19

152 ± 19a

  Moving time (s)

3668 ± 432

3424 ± 775

2974 ± 359

2204 ± 362a

Epididymal fat

  Weight (mg)

624 ± 74

566 ± 90

408 ± 58

262 ± 26a,b

  Adipocyte area (μm2)

2395 ± 147

1920 ± 160

1851 ± 300

1690 ± 152a

Mesenteric fat

  Weight (mg)

298 ± 31

277 ± 19

268 ± 22

205 ± 16a,b

  Adipocyte area (μm2)

1217 ± 84

1136 ± 136

1315 ± 153

997 ± 108a,c

Subcutaneous fat

  Weight (mg)

284 ± 26

269 ± 21

233 ± 20

208 ± 10a

  Adipocyte area (μm2)

1356 ± 60

1055 ± 69a

1188 ± 2

1069 ± 124a

Interscapular brown fat

  Weight (mg)

173 ± 10

121 ± 5a

119 ± 4.8a

76 ± 5a,b,c

Liver

  Weight (mg)

1148 ± 59

1233 ± 18

1134 ± 26

1009 ± 36b

Plasma

  TG (mmol/l)

1.24 ± 0.11

0.62 ± 0.06a

0.65 ± 0.06a

0.35 ± 0.05a,b,c

  β-Hydroxybutyrate (mmol/l)

0.09 ± 0.01

0.13 ± 0.02a

0.20 ± 0.01a,b

0.28 ± 0.02a,b,c

  Glucose (mmol/l)

17.2 ± 0.5

17.0 ± 0.4

17.4 ± 0.5

15.5 ± 0.8

  Insulin (pmol/l)

300 ± 35

219 ± 37a

57 ± 5a,b

35 ± 9a,b,c

  Adiponectin (μg/ml)

8.9 ± 0.3

12.1 ± 0.5a

9.3 ± 0.2b

11.1 ± 0.5a,c

  Leptin (ng/ml)

77.2 ± 7.6

66.2 ± 8.3a

15.5 ± 1.7a,b

5.3 ± 0.7a,b,c

All the variables were evaluated after 5 weeks of the treatment, except for physical activity, which was evaluated during week 4 and is represented by the total distance moved and moving time during both time intervals of the measurement (2 h during light phase and 2 h during dark phase, see Methods). In the case of WAT, subcutaneous dorsolumbar, epididymal and mesenteric depots were collected. Tissue dissection and plasma collection were performed in random-fed state (between 08:00 hours and 10:00 hours)

Data are means ± SEM; n = 10

a,b,cSignificant differences (ANOVA) compared with cHF, cHF + F, and cHF + CR, respectively

Combination treatment improves lipid and glucose homeostasis and preserves metabolic flexibility

After 5 weeks of the treatment and in the ad libitum fed state, plasma TG levels were strongly reduced through either LC n-3 PUFA or calorie restriction alone, and even more with the combination treatment (Table 1). This hypolipidaemic effect was negatively correlated with the effect of the treatments on plasma β-hydroxybutyrate, the marker of lipid catabolism. Glucose levels in plasma were similar in all the groups; however, insulin levels were decreased by cHF + CR treatment and even more by the cHF + F + CR treatment (Table 1). In this respect, cHF + F exerted a smaller but still significant effect. In the fasted state (Fig. 1g), the plasma concentration of glucose was lowered in response to calorie restriction, independent of dietary LC n-3 PUFA, with a trend for the lowest value in the cHF + F + CR mice. Levels of adiponectin were increased by cHF + F to a similar extent in mice with free access to food and in the mice with restricted energy intake (Table 1), also in agreement with our previous findings [27]. As expected from the effects of treatments on adiposity, leptin levels tended to be reduced by cHF + F and were significantly lowered by calorie restriction, with the cHF + F + CR mice showing the lowest leptin levels (Table 1).

To characterise whole-body metabolic flexibility, which is associated with insulin sensitivity [2, 32], the metabolic response to the FASTED/RE-FED transition was also analysed by assessing plasma levels of NEFA and glucose after 4 weeks of the treatment (Fig. 1f,g and ESM Fig. 1). Only in the FASTED, and not in the RE-FED state, did the metabolite levels differ between groups. Among the mice fed cHF-based diets, the cHF + F + CR mice showed the highest NEFA and the lowest glucose levels, while exhibiting the largest differences in plasma levels of both metabolites in response to the re-feeding. In fact, the cHF + F + CR treatment normalised the metabolic response when compared with the Chow-fed mice (Fig. 1f,g). In contrast, the cHF mice showed the lowest NEFA and the highest glucose levels in fasted state, with a minimum response to the FASTED/RE-FED transition observed with both metabolites (Fig. 1f,g). Indirect calorimetry was performed after 3 weeks of the treatment (ESM Figs 1 and 2) to characterise metabolic flexibility, as well as whole-body capacity to use carbohydrate and lipid fuels (Fig. 1h). When metabolic flexibility was expressed as maximal induction of RQ in response to the intragastric gavage of glucose, the combination treatment preserved metabolic flexibility better than any of the treatments applied separately. No effect of any of the treatments on energy expenditure, assessed as oxygen consumption (\( \dot{V}{{\text{O}}_{{2}}} \))/animal, was observed either before or after the glucose gavage (ESM Fig. 2 and ESM Table 1).

To further characterise changes in glucose homeostasis, fasted plasma was analysed (Fig. 2a,b) and an OGTT was performed (Fig. 2c and ESM Fig. 3) after 3–4 weeks of treatment (ESM Fig. 1). Compared with cHF diet, only the combination treatment significantly decreased fasted insulinaemia and HOMA index (Fig. 2a,b). Specifically in mice subjected to calorie restriction, glucose levels were increased in response to overnight fasting, which preceded the glucose tolerance test (ESM Fig. 3). Feeding cHF diet resulted in glucose intolerance (Fig. 2c), while the deterioration of glucose homeostasis was prevented by the combination treatment with the other treatments tending to exert similar effects. In addition, insulin levels estimated in plasma during the test, 30 min after the glucose gavage, revealed the protective effect of the combination treatment (220 ± 51, 296 ± 37, and 253 ± 41 pmol insulin/l in the Chow-fed, cHF and cHF + F + CR mice, respectively). Ex vivo analysis of insulin-stimulated de novo FA synthesis in epididymal fat after 5 weeks of treatment showed an increased rate of FA synthesis due to either LC n-3 PUFA or calorie restriction alone, and a synergistic induction in response to the combination treatment (Fig. 2d).
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Fig. 2

Improvement of glucose homeostasis. a Insulin levels in plasma and b HOMA index of insulin resistance after 4 weeks of the treatment, calculated (see ESM) from the insulin levels (see a) and glycaemia measured in the fasted state (see Fig. 1g); c OGTT after 3 weeks of treatment (0.5 ml 30% [wt/vol.] d-glucose in water; see ESM Fig. 3). The results are expressed as AUC values; d ex vivo analysis of de novo FA synthesis in epididymal fat after 5 weeks of the treatment, measured in the presence of insulin (80 μU/ml) and expressed as dpm 3H incorporated to saponifiable FAs/mg tissue. White bars, cHF or cHF + CR; black bars, cHF + F or cHF + F + CR; grey bars, Chow. Data are means ± SEM. a,b,cSignificant difference (ANOVA) compared with cHF, cHF + F, and cHF + CR, respectively; dsignificant difference (t test) compared with Chow-fed mice

Synergistic induction of genes of mitochondrial biogenesis and lipid catabolism in epididymal fat

Based on the previous results showing induction of mitochondria and lipid catabolism by LC n-3 PUFA [13] and calorie restriction [3], we investigated gene expression in WAT, interscapular brown fat, liver and muscle (Fig. 3a–e). In epididymal WAT (Fig. 3a), the analysis revealed a strong induction of PPARγ co-activator 1α (Pgc-1α [also known as Ppargc1a]), peroxisome proliferator-activated receptor α (Pparα [also known as Ppara]), nuclear respiratory factor 1 (Nrf1) and CyOX subunit 3 (Cyox3 [also known as Cox3]) by the combination treatment, suggesting increased biogenesis and oxidative capacity of mitochondria, independent of the type of diet (see legend for Fig. 3). Only Pparα expression was upregulated in subcutaneous WAT by the combination treatment, while no significant changes in the expression of the above gene cluster was observed in interscapular brown fat (Fig. 3c), liver (Fig. 3d) or skeletal muscle (Fig. 3e). Concerning the genes of β-oxidation such as very long-chain, long-chain and medium-chain acyl-CoA dehydrogenases (Vlcad [also known as Acadvl], Lcad [also known as Acadl] and Mcad [also known as Acadm], respectively), additive induction of Vlcad and Lcad was noted in the epididymal WAT (Fig. 3a), and a slight increase in the expression of the β-oxidation genes observed in response to cHF + F or cHF + F + CR treatments in the liver (Fig. 3d) but not in the muscle (Fig. 3e). FA synthase (Fas) was upregulated in epididymal WAT of the cHF + F + CR mice (Fig. 3a), but significant downregulation of Fas was observed in the liver of these animals (Fig. 3d). No induction of uncoupling protein 1 (Ucp1) by any of the treatments either in epididymal fat or in interscapular brown fat was detected (Fig. 3a,c).
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Fig. 3

Specific induction of mitochondrial genes in epididymal fat. Quantitative real-time RT-PCR data showing relative levels of gene expression (cHF = 1) in epididymal (a) and subcutaneous (b) WAT, interscapular brown fat (c), liver (d), and gastrocnemius muscle (e) after 5 weeks of the treatment. Data are means ± SEM; n = 10; white bars, cHF; black bars, cHF + F; light grey bars, cHF + CR; dark grey bars, cHF + F + CR; a,b,cSignificant difference (ANOVA) compared with cHF, cHF + F and cHF + CR, respectively. An independent experiment showed no difference in either Pgc-1α or Pparα expression in epididymal fat between the cHF and the Chow-fed mice (the cHF/Chow ratio of the Pgc-1α and Pparα transcript levels was 1.08 ± 0.05 and 1.04 ± 0.08, respectively; n = 6). All analyses were performed after 5 weeks of treatment

Combination treatment induced FA oxidation and mitochondrial oxidative capacity in epididymal fat

To confirm the synergistic induction of mitochondrial FA oxidation by LC n-3 PUFA and calorie restriction in epididymal fat, biochemical assessment was performed ex vivo after 5 weeks of the dietary treatment. First, [1-14C]palmitate oxidation was measured in fragments of epididymal fat. Feeding the cHF diet stimulated palmitate oxidation compared with Chow feeding, and this activity was further significantly stimulated only by the combination treatment, documenting a synergism between LC n-3 PUFA and calorie restriction in the induction of FA oxidation (Fig. 4a). Accordingly, adipocytes isolated from epididymal fat of the cHF + F + CR mice also exerted a higher DNA-adjusted rate of palmitate oxidation compared with cHF mice (Fig. 4b). This analysis, as well as the analysis of mitochondrial function described below, was restricted to the comparison between the cHF mice and the cHF + F + CR mice, i.e. the two groups supposed to exhibit the largest differences in the metabolic variables.
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Fig. 4

Induction of fatty acid oxidation and mitochondrial oxidative capacity in epididymal fat. a Oxidation of [1- 14C]palmitate into CO2 by tissue fragments. Data are means ± SEM; n = 10. White bars, cHF or cHF + CR; black bars, cHF + F or cHF + F + CR; grey bars, Chow. aSignificant difference (ANOVA) compared with cHF; dsignificant difference (t test) compared with Chow-fed mice. b Oxidation of [1-14C]palmitate into CO2 by freshly isolated adipocytes. Data are means ± SEM; n = 8. aSignificant difference (t test). c, d, e Evaluation of mitochondrial respiratory capacity and oxidative phosphorylation in permeabilised adipocytes isolated from epididymal fat of the cHF and cHF + F + CR mice using multiple substrate-inhibitor titration respirometry. c, d Representative oxygen flux curves (c cHF; d cHF + F + CR). Adipocyte added in suspension (0.2 ml) to 2 ml incubation medium (10 mmol/l Tris-HCl, 80 mmol/l KCl, 3 mmol/l MgCl2, 5 mmol/l KH2PO4, 1 mmol/l EDTA and 1% wt/wt fatty-acid-free BSA, pH 7.4) were permeabilised with the addition of 3 μl digitonin (10 mg/ml DMSO), mitochondrial complex I was inhibited by 2 μl of 1 mmol/l rotenone, and respiration was stimulated by successive additions of: (1) 20 μl 1 mol/l glycerol 3-phosphate; (2) 20 μl 1 mol/l succinate; and (3) 10 μl 0.3 mol/l ADP (before the ADP addition, mitochondrial integrity was tested by addition of 10 μl of 4 mmol/l reduced cytochrome c). Subsequently, ATP synthase was inhibited by 2 μl oligomycin (4 mg/ml), respiration was re-activated by additions of uncoupler of oxidative phosphorylation (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; FCCP; 0.5 μl of 1 mmol/l FCCP at each addition), and finally, respiration was inhibited by 2 μl of 5 mmol/l antimycin A (inhibitor of complex III). e Averaged values from respirometry (n = 4; pooled samples from two animals). White bars, cHF; black bars, cHF + F + CR. Data are means ± SEM. aSignificant difference (paired t test) between the groups. All analyses were performed after 5 weeks of treatment

Second, the activity of CyOX, the terminal component of the mitochondrial respiratory chain, and the content of cytochrome b were evaluated in epididymal fat. In the cHF + F + CR mice, the CyOX activity was higher compared with cHF mice (4.5 ± 0.3 vs 3.5 ± 0.4 μmol cytochrome c oxidised min1 mg protein1, p < 0.05), in accordance with the difference in the cytochrome b content (926 ± 116 vs 590 ± 34 pmol/mg protein, p < 0.05).

Third, mitochondrial oxidative capacity was characterised in adipocytes using respirometry and adjusted to DNA content (Fig. 4c–e). Adipocytes from the cHF + F + CR mice exerted an approximately twofold higher rate of ADP-dependent oxygen consumption in the presence of respiratory substrates glycerol-3-phosphate and succinate (state 3 respiration) compared with the cHF mice. This indicates a much higher oxidative capacity of adipose tissue mitochondria in the cHF + F + CR mice. In addition, a similar degree of inhibition of state 4 respiration by oligomycin (Fig. 4c–e), similar respiratory control indexes (state 3/state 4; cHF + F + CR, 5.54 ± 1.13 vs cHF, 5.57 ± 1.15), and a reversal of the inhibitory effect of oligomycin by carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, see Fig. 4c,d) indicated tight coupling between respiration and phosphorylation in the epididymal fat mitochondria of both groups.

To assess changes in lipid catabolism in the other tissues, palmitate oxidation was measured in isolated hepatocytes and in skeletal muscles ex vivo, following the 5 week treatment (ESM Table 2). The combination treatment, but not the other treatments, increased the hepatic FA oxidation compared with the cHF mice, but it had no effect on FA oxidation in muscle.

Combination treatment ameliorates low-grade adipose tissue inflammation induced by high-fat diet

As LC n-3 PUFA prevented obesity-associated low-grade inflammation of WAT in mice [15, 16], we analysed the anti-inflammatory action of LC n-3 PUFA in the context of mild restriction of energy intake. Immunohistochemical analysis of epididymal fat of various treatments revealed a reduced content of macrophages aggregated in crown-like structures (CLS) surrounding dead adipocytes [33] compared with the cHF mice. The macrophage infiltration was maximally reduced in mice subjected to the combination treatment (Fig. 5a–c) and mRNA levels for the macrophage marker CD68 were decreased in the epididymal fat of all treatment groups compared with the cHF controls (Fig. 3a). Moreover, plasma levels of IL-6, a marker of systemic inflammation, were significantly reduced only by the combination treatment (Fig. 5d).
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Fig. 5

Prevention of adipose tissue and systemic inflammation. Immunohistochemical analysis of epididymal fat of the cHF mice (a) and the cHF + F + CR mice (b) with visualised MAC-2 antigen (expressed on activated macrophages); arrow indicates aggregates of macrophages forming CLS, which surround dead adipocytes. c Relative count of CLS; n = 6. d Plasma level of IL-6, marker of systemic inflammatory status. Data are means ± SEM; n = 10; white bars, cHF or cHF + CR; black bars, cHF + F or cHF + F + CR; aSignificant difference (ANOVA) compared with the cHF mice. The morphometry data are based on more than 1,000 cells taken randomly from six different areas per animal. All analyses were performed after 5 weeks of the treatment

Specific induction of anti-inflammatory lipid mediators in epididymal fat

To investigate the possible involvement of bioactive lipids derived from PUFA and modulating inflammation (see Introduction and Fig. 6), lipidomic analysis was performed in epididymal fat and in the liver. In total, 24 lipid species were quantified (ESM Table 3). Partial least-squares-discriminant analysis (PLS-DA) revealed the major discriminating lipid species between the groups. In WAT, but not in the liver, the first two PLS-DA components separated mice into four distinct groups corresponding to dietary treatments (ESM Figs 4 and 5). Following contribution-score analysis, the most important lipids were identified for each of the intervention groups. As shown in Fig. 7a,c,e and in ESM Table 3, the levels of AA, EPA and DHA in the free FA fraction of WAT were significantly higher in all treated groups compared with the cHF mice (except for AA in the ad libitum mice treated by LC n - 3 PUFA). The cHF + F + CR treatment exerted the most pronounced effect. In the liver, free EPA and DHA levels were significantly affected only by LC n-3 PUFA, independent of calorie restriction (Fig. 7d,f). In both tissues, the levels of primary monohydroxy metabolites derived from linoleic (HODE), AA (HETE), EPA (HEPE) and DHA (HDoHE) correlated with those of the corresponding FAs (Fig. 6). The lipoxygenase (LOX) products were the most abundant autacoids in both tissues of the cHF controls. Dietary supply of EPA and DHA triggered formation of LC n-3 PUFA-derived metabolites in both tissues. The WAT-specific liberation of FAs from membrane phospholipids in response to calorie restriction resulted in the formation of lipid mediators derived from both LC n-3 and LC n-6 PUFA (Fig. 6). Only in WAT, dietary LC n-3 PUFA combined with calorie restriction synergistically increased the levels of protectin D1 (PD1; Fig. 7g), a well described anti-inflammatory lipid mediator derived from DHA, or PD1 isomers such as PDX. In accordance with a previous finding [18], resolvin E1 (derived from EPA) was below the level of detection. Unexpectedly, 15-deoxy-Δ12,15-prostaglandin J2 (15d-PGJ2), an anti-inflammatory mediator and potent PPARγ agonist derived from AA [34], was also synergistically upregulated by the combination treatment specifically in WAT (Fig. 7i).
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Fig. 6

Overview of the effects of calorie restriction, LC n-3 PUFA and the combination treatment on the levels of lipid mediators in epididymal fat. Scheme is based on the results of lipidomic analysis (see Fig. 7, ESM Table 3 and ESM Fig. 3). Significantly upregulated metabolites are marked by different colours; red colour, upregulated by calorie restriction; blue colour, upregulated by LC n-3 PUFA; green colour, upregulated by each of the treatments; asynergistic upregulation by the combination treatment. Some pathways mentioned in this figure are speculative and not conclusively described. The scheme is inspired by Larsson et al. [50]. ALA, α-linolenic acid (18:3 n-3); DGLA, dihomo-γ-linolenic acid (20:3 n-6); EFOX, electrophilic oxo-derivatives from DHA [19]; GLA, γ-linolenic acid (18:3 n-6); HXB3, hepoxilin B3; LA, linoleic acid (18:2 n-6); 5-,12-,15-LOX, 5,12,15-lipoxygenase; LTB4/B5, leukotriene B4/B5; PD1, protectin D1; PG, prostaglandin; PLA2, phospholipase A2; Rev-E1, resolvin E1; TXB2, thromboxane B2

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Fig. 7

Synergistic induction of anti-inflammatory lipid mediators in adipose tissue. a–j Selected results of lipidomic HPLC MS-MS analysis of epididymal fat (a, c, e, g, i) and liver (b, d, f, h, j) after 5 weeks of treatment. a, b AA; c, d EPA; e, f DHA; g, h PD1; i, j 15d-PgJ2. Data are expressed as relative concentration of individual lipids adjusted to wet weight (cHF = 1) and expressed as means ± SEM; n = 3. White bars, cHF or cHF + CR; black bars, cHF + F or cHF + F + CR. a,b,cSignificant differences (ANOVA) compared with cHF, cHF + F, cHF + CR, respectively. For complete results, see ESM Table 3

Discussion

We show here that the combination treatment of dietary LC n-3 PUFA and mild calorie restriction is more effective than each of the treatments applied separately in the protection against obesity in mice. Pronounced induction of mitochondrial lipid catabolism and synergistic induction of anti-inflammatory lipid mediators in WAT could contribute the systemic effects of the combination treatment (Fig. 8).
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Fig. 8

Putative involvement of AMPK/SIRT1 signalling, 15d-PGJ2 and PD1 in the additive metabolic and anti-inflammatory effects of the combination of LC n-3 PUFA and calorie restriction in adipose tissue. Dietary intake of LC n-3 PUFA combined with calorie restriction activates AMPK/SIRT1 signalling pathway and synergistically induces production of anti-inflammatory and pro-resolving lipid mediators by COX/LOX enzymatic systems. AMPK and SIRT1 can increase activity of transcriptional co-activator PGC-1α by phosphorylation and de-acetylation, respectively. PGC-1α can enhance transcriptional activity of PPAR nuclear receptors, resulting in higher mitochondrial oxidative capacity and induction of FA catabolism in situ in white fat. Simultaneously, unsaturated FAs (light green circle) as well as their active metabolites (lipid mediators) can also activate directly PPARα and PPARγ (e.g. 15d-PGJ2). Lipid mediators are formed after the release of FAs from membrane phospholipids and can inhibit the NF-κB signalling pro-inflammatory pathway (15d-PGJ2, PD1) or induce the resolution phase of inflammation (PD1). Of note, SIRT1 can also interfere with NF-κB signalling. AC, acetyl group; P, phosphate

Our results demonstrate an improvement of metabolic flexibility by the combination treatment, based on both the response of plasma levels of NEFA and glucose to FASTED to RE-FED transition and the glucose-induced increase in RQ. This improvement of metabolic flexibility correlated with changes in body weight, adiposity and glycaemic control in accordance with the notion that metabolic flexibility is closely associated with insulin sensitivity [2, 32] and that impaired flexibility represents an early defect in the development of type 2 diabetes [35].

Metabolic syndrome is linked to inflammatory changes in both WAT and liver [1]. In this study, in accordance with the previous findings [17, 18, 21], dietary LC n-3 PUFA supplementation resulted in the inhibition of formation of various LC n-6 PUFA-derived pro-inflammatory eicosanoids in both tissues as well as in the induction of the anti-inflammatory molecules. In WAT, in contrast to the liver, the levels of EPA, DHA, AA and their active metabolites, including the anti-inflammatory molecules PD1 and prostaglandin 15d-PGJ2, were increased in a synergistic manner by the combination treatment (Fig. 7). A key role of the enzyme involved in PD1/PDX formation, 12/15 LOX, in adipose tissue inflammation and insulin resistance has been described [36, 37]. The induction of prostaglandin 15d-PGJ2 was an unexpected finding as 15d-PGJ2 is derived from the metabolism of LC n-6 PUFA, and enzymatic formation of LC n-6 PUFA metabolites is, in general, inhibited upon LC n-3 PUFA supplementation. This suggests that dietary LC n-3 PUFA, especially in combination with calorie restriction, selectively activates formation of 15d-PGJ2 from prostaglandin D2 (PGD2), the major product of cyclooxygenase (COX) in many tissues [34], reflecting possibly the ability of EPA- and DHA-derived peroxyl radicals to favour the formation of ‘less pro-inflammatory’ peroxidation products derived form AA [38].

Here we suggest for the first time that 15d-PGJ2 represents an important mediator of LC n-3 PUFA effects. 15d-PGJ2 can modulate the activity of various signalling molecules by covalent binding and their subcellular localisation [34], including various components of the NF-κB signalling pathway [19, 39]. Moreover, 15d-PGJ2 is the most potent endogenous ligand for PPARγ [40, 41]. Interestingly, DHA is a precursor for anti-inflammatory electrophilic cyclopentenone neuroprostanes in macrophages [20] and electrophilic oxo-derivatives generated by COX-2 reactions [19], which can act similarly to 15d-PGJ2. Thus, the activation of PPARγ via 15d-PGJ2 binding could be responsible for the additive effects of the combination treatment on adipose tissue energy and lipid metabolism. This idea is supported by the fact that rosiglitazone, a PPARγ ligand, increases PPARγ coactivator 1α (PGC-1α) production, mitochondrial mass, palmitate oxidation and mitochondrial uncoupling protein 1 (UCP1) in the adipose tissue of genetically obese ob/ob mice [42].

It has been found that brown adipocyte-like cells originating from rosiglitazone-treated epididymal WAT cell precursors represent a new subtype of adipocytes, called ‘brite’ cells, which differ from classic white and brown adipocytes, but possess mitochondrial UCP1-mediated thermogenesis [43]. Recent studies [44, 45] demonstrated the involvement of COX-2 in the induction of these fat-burning cells and the importance of the COX-2-mediated mechanisms for the resistance to dietary obesity in mice. Our results convincingly demonstrate a marked induction of mitochondrial oxidative capacity in permeabilised adipocytes isolated from epididymal fat in response to mild calorie restriction combined with LC n-3 PUFA intake, using succinate as the optimum fuel [46]. Moreover, changes in palmitate oxidation in both intact isolated adipocytes and in fat fragments document induction of energy expenditure by the combination treatment. Importantly, these changes occurred even in the absence of UCP1, depending probably on the simultaneous activation of PPARα/PGC-1α and PPARγ signalling (Fig. 8). Induction of futile substrate cycling in adipocytes by this mechanism [7] might explain the increased lipid catabolism in the absence of mitochondrial uncoupling. This idea is consistent with our current findings that the combination treatment increases capacity for de novo lipogenesis in epididymal fat, both at the level of gene expression and biochemical activity. In adult mice reared at 20°C, total oxidative capacity in WAT represents ∼30–50% of brown adipose oxidative capacity [10] indicating that energy expenditure in WAT may influence total energy balance (see Introduction). Even small but persistent changes in WAT energy expenditure, undetectable using indirect calorimetry, could influence body weight substantially. Fat-depot-specific differences affecting the inducibility of energy-dissipating adipocytes should be explored further. In addition to the stimulation of lipid catabolism in WAT, FA oxidation in liver—but not in skeletal muscle—could contribute to the whole-body effects of the combination treatment.

We have demonstrated previously that AMP-activated protein kinase (AMPK), a cellular energy sensor, is involved in the effects of LC n-3 PUFA on hepatic lipid and glucose metabolism [30] and that it is activated in WAT by LC n-3 PUFA [47]. AMPK activation in adipocytes results in increased mitochondrial biogenesis and lipid catabolism [4, 48]. Moreover, AMPK closely interacts with deacetylase sirtuin 1 (SIRT1), which controls metabolic processes in response to calorie restriction and exerts anti-inflammatory effects in WAT [49]. Therefore, AMPK/SIRT1 signalling is probably involved in the additive metabolic and anti-inflammatory effects of the combination treatments in epididymal WAT (Fig. 8).

In summary, dietary LC n-3 PUFA augment the anti-obesity effects of mild calorie restriction in mice while improving lipid metabolism and glucose homeostasis. These effects are probably reflected by the large synergistic induction of mitochondrial FA oxidation in WAT, linked to a suppression of low-grade inflammation of this tissue. The synergistic induction of specific anti-inflammatory lipid mediators, namely 15d-PGJ2, the LC n-6 PUFA metabolite, and PD1, the LC n-3 PUFA metabolite, may underlie both the anti-inflammatory and metabolic effects of the combination treatment in WAT. Further exploration of the strategy to target WAT by combining two complementary and physiological approaches, i.e. dietary intake of LC n-3 PUFA and mild restriction of energy intake, may be valuable for the prevention and treatment of metabolic syndrome.

Acknowledgements

This work was supported by the research project of the Ministry of Education, Youth and Sports (1M6837805002) and by the Czech Science Foundation (303/08/0664). Further support includes a grant from EPAX A.S. (Aalesund, Norway), COST action FA0602 and the research project AV0Z 50110509. We thank J. Bemova, S. Hornova and D. Salkova (Institute of Physiology AS CR, Prague) for technical assistance and U. Jahn (Institute of Organic Chemistry and Biochemistry AS CR, Prague, Czech Republic) for critical reading of the manuscript.

P.F. contributed to the conception and design of experiments, analysis and interpretation of data, and drafted the manuscript; R.R. and M.R. contributed to the conception and design of experiments, analysis and interpretation of data, and revising the manuscript; M.H., P.J., P.Z., V.K., Z.M.J., E.P., O.K., M.S., G.T. and V.M-A. contributed to the analysis and interpretation of data, and revising the manuscript; and J.K. coordinated the research, contributed to the conception and design of experiments, and drafted the manuscript. All authors approved the final version of the manuscript.

Duality of interest

The authors declare that there is no duality of interest associated with this manuscript.

Supplementary material

125_2011_2233_MOESM1_ESM.pdf (169 kb)
ESM Methods(PDF 168 kb)
125_2011_2233_MOESM2_ESM.pdf (10 kb)
ESM Table 1Indirect calorimetry data evaluation (PDF 9 kb)
125_2011_2233_MOESM3_ESM.pdf (12 kb)
ESM Table 2Palmitate oxidation rates in liver and skeletal muscle (PDF 12 kb)
125_2011_2233_MOESM4_ESM.pdf (19 kb)
ESM Table 3HPLC MS/MS quantification of lipid-derived mediators in adipose tissue and liver (PDF 18 kb)
125_2011_2233_MOESM5_ESM.pdf (12 kb)
ESM Figure 1Overview of experimental setup. Scheme of individual dietary experiments, which lasted for either 5 for 15 weeks (PDF 11 kb)
125_2011_2233_MOESM6_ESM.pdf (47 kb)
ESM Figure 2Indirect calorimetry. Oxygen consumption (VO2) and carbon dioxide production (VCO2) were recorded in singly-caged mice every 2 min at 30°C. a Levels of substrate partitioning were estimated by calculating respiratory quotient (RQ; i.e. VCO2/ VO2). Metabolic flexibility was assessed as a maximal induction in RQ values in response to a glucose load (0.45 ml of 50% D-glucose) administered by intragastric gavage to overnight (12 hours) fasted animals (Time 0). The induction was calculated from RQ values averaged over 60-min-intervals before and after gavage, respectively (indicated by horizontal bars). Black line-cHF; blue line- cHF+F; red line- cHF+CR; green line- cHF+F+CR. bVO2 before and after gavage. Data are means±SE. Mice were analyzed during the fourth week of the treatment, i.e. before any significant differences in body weights between the treatments appeared. For evaluation of calorimetric data see ESM Table 1 (PDF 47 kb)
125_2011_2233_MOESM7_ESM.pdf (9 kb)
ESM Figure 3An oral glucose tolerance test was performed after overnight fasting (15–16 h). White circles- cHF; black circles- cHF+F; white inverted triangles- cHF+CR; black inverted triangles- cHF+F+CR; grey circles- Chow (PDF 9 kb)
125_2011_2233_MOESM8_ESM.pdf (15 kb)
ESM Figure 4Partial least squares-discriminant analysis (PLS-DA) of lipidomic data in adipose tissue. In total, 24 lipid molecular species were quantified in epididymal fat using HPLC MS-MS after 5 weeks of the treatment (ESM Table 3). To identify the major lipid species discriminating between the groups, PLS-DA was performed using Umetrics SIMCA-P+12 statistical software. a 2D-scatter plots of the first (axis X) and the second (axis Y) PLS-DA component. Black circle- cHF; blue circle- cHF+F; red circle- cHF+CR; green circle- cHF+F+CR. b Contribution score analysis using the corresponding linear combination of the p vector as weights in the epididymal fat of mice fed respective diets. The dominating bars indicate which variables deviate the most from the average, and the sign of the bar (down and up means minus and plus, respectively) indicate in which direction the variables deviate (PDF 14 kb)
125_2011_2233_MOESM9_ESM.pdf (15 kb)
ESM Figure 5PLS-DA of lipidomic data in liver. In total, 24 lipid species were quantified in liver after 5 weeks of the treatment (see ESM Table 3) and data were analyzed as in ESM Fig 3. Black circle- cHF; blue circle- cHF+F; red circle- cHF+CR; green circle- cHF+F+CR (PDF 15 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • P. Flachs
    • 1
  • R. Rühl
    • 2
    • 3
  • M. Hensler
    • 1
  • P. Janovska
    • 1
  • P. Zouhar
    • 1
  • V. Kus
    • 1
  • Z. Macek Jilkova
    • 1
  • E. Papp
    • 2
    • 3
  • O. Kuda
    • 1
  • M. Svobodova
    • 1
  • M. Rossmeisl
    • 1
  • G. Tsenov
    • 4
  • V. Mohamed-Ali
    • 5
  • J. Kopecky
    • 1
  1. 1.Department of Adipose Tissue BiologyInstitute of Physiology Academy of Sciences of the Czech Republic v.v.i.PragueCzech Republic
  2. 2.Department of Biochemistry and Molecular Biology, Laboratory of Nutritional Bioactivation and BioanalysisUniversity of DebrecenDebrecenHungary
  3. 3.Apoptosis and Genomics Research Group of the Hungarian Academy of ScienceDebrecenHungary
  4. 4.Department of Developmental EpileptologyInstitute of Physiology, Academy of Sciences of the Czech Republic v.v.i.PragueCzech Republic
  5. 5.Adipokines and Metabolism Research GroupUniversity College LondonLondonUK