Differential effects of monounsaturated, polyunsaturated and saturated fat ingestion on glucose-stimulated insulin secretion, sensitivity and clearance in overweight and obese, non-diabetic humans
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- Xiao, C., Giacca, A., Carpentier, A. et al. Diabetologia (2006) 49: 1371. doi:10.1007/s00125-006-0211-x
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Prolonged elevation of plasma specific fatty acids may exert differential effects on glucose-stimulated insulin secretion (GSIS), insulin sensitivity and clearance.
Subjects and methods
We examined the effect of oral ingestion, at regular intervals for 24 h, of an emulsion containing either predominantly monounsaturated (MUFA), polyunsaturated (PUFA) or saturated (SFA) fat or water (control) on GSIS, insulin sensitivity and insulin clearance in seven overweight or obese, non-diabetic humans. Four studies were conducted in each individual in random order, 4–6 weeks apart. Twenty-four hours after initiation of oral ingestion, subjects underwent a 2 h, 20 mmol/l hyperglycaemic clamp to assess GSIS, insulin sensitivity and insulin clearance.
Following oral ingestion of any of the three fat emulsions over 24 h, plasma NEFAs were elevated by ∼1.5- to 2-fold over the basal level. Ingestion of any of the three fat emulsions resulted in reduction in insulin clearance, and SFA ingestion reduced insulin sensitivity. PUFA ingestion was associated with an absolute reduction in GSIS, whereas insulin secretion failed to compensate for insulin resistance in subjects who ingested SFA.
Oral ingestion of fats with differing degrees of saturation resulted in different effects on insulin secretion and action. PUFA ingestion resulted in an absolute reduction in insulin secretion and SFA ingestion induced insulin resistance. Failure of insulin secretion to compensate for insulin resistance implies impaired beta cell function in the SFA study.
KeywordsInsulin clearanceInsulin resistanceInsulin secretionLipotoxicityNon-esterified fatty acidsNEFAsOral fat ingestion
glucose infusion rate
glucose-dependent insulinotropic polypeptide
glucose-stimulated insulin secretion
plasma insulin concentration during the last 30 min of the hyperglycaemic clamp
insulin secretion rate
monounsaturated fatty acid
polyunsaturated fatty acid
saturated fatty acid
Type 2 diabetes is characterised by defects in both insulin secretion and insulin action [1, 2], with a specific defect in glucose-stimulated insulin secretion (GSIS) early in the evolution of this disease [3, 4]. While it has been generally accepted that fatty acids are essential for maintaining basal insulin secretion  and that an acute increase in plasma NEFAs enhances GSIS, prolonged elevation of plasma NEFAs has been shown to desensitise GSIS in humans in vivo (reviewed in [6, 7]), particularly in predisposed individuals . These findings are supported by a large body of in vitro work (reviewed in ).
The acute enhancement of GSIS by i.v. infusion of fat in rats depends on the type of NEFA, being proportional to the NEFA chain length and degree of saturation . In humans, short-term oral ingestion of monounsaturated fat is more potent than ingestion of polyunsaturated and saturated fats in stimulating GSIS . However, it remains unclear whether the saturation of fatty acids affects GSIS when levels are chronically elevated in vivo in humans. Much of the data from human studies to date come from infusion studies in which i.v. fat emulsions (Intralipid or Liposyn) and heparin were administered to provide an exogenous source of triglycerides and to stimulate lipoprotein lipase, respectively. The effect of saturated fatty acids (SFAs) or monounsaturated fatty acids (MUFAs) on beta cell function may differ from that of the largely polyunsaturated fatty acids (PUFAs) in these lipid emulsions. In addition, dietary NEFAs also stimulate intestinal secretion of incretins such as glucagon-like-peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), and can therefore indirectly potentiates GSIS . Infusion of heparin–Intralipid to induce elevation of plasma NEFAs bypasses the gastrointestinal tract, which limits the use of this experimental system in examining the effect of dietary fatty acids on insulin action and sensitivity. For these reasons, and because solutions of MUFA and SFA are not currently available for safe i.v. administration to humans, in the present study we compared the effects of oral ingestion of SFA, PUFA and MUFA on insulin secretion and sensitivity.
The composition of plasma NEFAs was altered in the present study by the frequent ingestion of a hot, chocolate-flavoured drink of fat emulsion that was virtually free of carbohydrate and protein, according to the method described recently by Beysen et al. . In pilot studies we determined the maximal dose and duration of fat that could be administered in this fashion without causing nausea or diarrhoea. The objective of this study was to investigate the effects of the ingestion of various fatty acids, i.e. saturated, monounsaturated and polyunsaturated, on GSIS, insulin sensitivity and insulin clearance in humans. We elected to study overweight and obese, non-diabetic humans because we have previously shown that they are more susceptible to the impairing effect of chronically elevated fatty acids on GSIS than lean, insulin-sensitive individuals .
Subjects and methods
Characteristics of the subjects
Fasting plasma glucose (mmol/l)
Fasting plasma insulin (pmol/l)
Fasting plasma NEFA (mmol/l)
Fasting plasma C-peptide (nmol/l)
Participants were admitted to the Endocrine/Metabolic Diagnostic Unit of the Toronto General Hospital at 07.00 h on four occasions, in randomised order, 4–6 weeks apart, after a 12 h overnight fast. On each occasion, participants ingested a hot, chocolate-flavoured drink consisting of either one of three fat emulsions (oil tests) or of water (control) every hour for the first 12 h followed by every 2 h for the remainder of the study, continuing through the clamp studies that began at 08.00 h on day 2 (i.e. the emulsion was ingested for a total of 30 h). For the three oil tests, the hot drink at each ingestion time point consisted of the following ingredients: 13 g oil, 0.5 g emulsifier (monoglycerides), 1 g sweetener, 3 g cocoa powder and 100 ml hot water. The macronutrient composition of this emulsion was 89.02% energy from fat, 9.24% from carbohydrate and 1.74% from protein (136 kcal or 571 kJ ingested at each time point). The emulsion was made up each day, kept warm in a Thermos flask and mixed by shaking prior to ingestion. The three oils used in the study were olive oil (78% monounsaturates, 8% polyunsaturates and 14% saturates; 71% oleic acid, 13% palmitic acid and 10% linoleic acid; referred to as MUFA), palm oil (50% saturates, 40% monounsaturates and 10% polyunsaturates; 48% palmitic acid, 35% oleic and 8% linoleic acid; SFA) and safflower oil (78% polyunsaturates, 13% monounsaturates and 9% saturates; 74% linoleic acid, 12% oleic acid and 6% palmitic acid; PUFA). We acknowledge that palm oil contains not only SFA but also a fair amount of MUFA. In the control test, oil was replaced with water. Subjects were not permitted to eat for the duration of the experiments but were allowed free access to water. Blood samples were drawn at 08.00, 12.00 and 16.00 h on day 1 and 08.00 h on day 2 for monitoring blood glucose, insulin, triglycerides, NEFA, C-peptide, GLP-1 and GIP. Ingestion of the emulsion began after the 08.00 h sample had been drawn on day 1.
Assessment of glucose-stimulated insulin secretion, insulin sensitivity and insulin clearance
On day 2, an i.v. catheter was placed in the superficial vein of one forearm for infusion of glucose and insulin and another catheter was placed in the other forearm for blood sampling. The arm in which the sampling catheter was placed was maintained in a heating blanket (∼65°C) to ‘arterialise’ venous blood. At approximately 08.00 h a 2 h, 20 mmol/l hyperglycaemic clamp study was conducted as previously described . Prior to starting the hyperglycaemic clamp, blood samples were drawn at 10 min intervals for analysis of glucose, insulin, triglycerides, NEFAs and C-peptide during a 30 min basal period before glucose infusion. Samples for NEFA and triglyceride analysis were collected into chilled EDTA-containing tubes on ice containing 30 μg/ml of the lipase inhibitor tetrahydrolipstatin (Orlistat; Hoffman La Roche, Mississauga, ON, Canada), to prevent ongoing in vitro lipolysis of the samples . The subjects then received a primed i.v. infusion of 20% dextrose for 2 h to rapidly achieve and then maintain hyperglycaemia of 20 mmol/l, as previously described . Blood samples were drawn every 5 min during the remainder of the clamp for glucose assay at the bedside. Samples for insulin, NEFAs and C-peptide were obtained 30 and 60 min into the clamp and every 5 min during the last 30 min of the clamp.
Laboratory methods (see Electronic Supplementary Material)
Insulin secretion rate
Insulin secretion rate (ISR) was calculated from peripheral venous plasma C-peptide levels by deconvolution using a two-compartment mathematical model with standard parameters for C-peptide distribution and metabolism as previously described .
Insulin clearance (ClI) was calculated as ClI=ISR/Insclamp, where Insclamp is plasma insulin concentration during the last 30 min of the hyperglycaemic clamp.
Estimation of insulin sensitivity
Since insulin levels during the last 30 min of the hyperglycaemic clamp varied significantly among treatments, it was assumed that, in the absence of changes in insulin sensitivity among treatments (null hypothesis), glucose infusion rates would exhibit an approximately linear relationship with clamp insulin levels, within the restricted range of insulin levels (from 1,000 to 2,000 pmol/l) observed in this protocol. The glucose infusion rate (Ginf) was plotted against clamp insulin level (Insclamp) to illustrate the effects of differences in fat ingestion on whole-body insulin action.
The data were expressed as mean±SEM. Levels of glucose, insulin, triglycerides, NEFA, C-peptide, specific fatty acids, GLP-1, GIP, cortisol and β-hydroxybutyrate at time intervals during the first 24 h were analysed by three-way ANOVA (independent variables: treatment, time and subject, with interaction between treatment and time) with post hoc Tukey’s t-test for multiple comparisons of means. The analysis was repeated for each time point. The comparison of plasma glucose, insulin and C-peptide concentrations, Ginf, ISR and ClI during the steady-state period (from 90 to 120 min of the hyperglycaemic clamp) was assessed by two-way ANOVA (independent variables: treatment and subject), with post hoc Tukey’s t-test for multiple comparisons of means to detect significant differences between treatments. A p value of <0.05 was considered significant. Statistical analyses were performed using SAS software, version 8.0 (SAS Institute, Cary, NC, USA).
Plasma glucose, insulin, triglycerides, NEFA, C-peptide, GLP-1 and GIP during fasting and ingestion of the emulsion for the first 24 h of the study
Plasma glucose, insulin and C-peptide levels during fasting and oral ingestion of control solution or SFA, MUFA or PUFA oil emulsion
Plasma NEFA and triglycerides during fasting and oral ingestion of control solution or SFA, MUFA or PUFA oil emulsion
GLP-1 and GIP levels during the 24 h ingestion period, prior to starting the clamp studies, were not different between studies (not illustrated). During this 24 h period the active GLP-1 levels in plasma did not increase from baseline in any of the studies (10.96±1.72 pmol/l for control, 9.86±4.09 pmol/l for SFA, 10.56±3.53 pmol/l for MUFA and 10.30±5.50 pmol/l for PUFA). Plasma GIP levels did not increase from baseline in the control group and even decreased slightly during the 24 h period (32.33±11.68 ng/l at 24 h versus 43.17±7.70 ng/l at baseline). Fat ingestion for 24 h resulted in increased levels of GIP in all treatment groups (74.14±10.75 ng/l at 24 h versus 40.00±7.52 ng/l at baseline for SFA, 98.00±13.14 vs 50.86±7.35 ng/l for MUFA, and 84.57±16.68 vs 51.50±15.14 ng/l for PUFA; p<0.05). GIP levels in all oil tests after 24 h of ingestion were higher than those in the control group (p<0.01 for SFA and MUFA, p<0.05 for PUFA).
Serum cortisol levels (not illustrated) were not different between treatment protocols prior to fat ingestion (330.4, 347.5, 364.5 and 337.4 nmol/l for control, SFA, MUFA and PUFA, respectively) and 24 h after fat ingestion (334.1, 328.6, 363.5 and 374.3 nmol/l for control, SFA, MUFA and PUFA, respectively). Ketones were not detected in plasma either at baseline or 24 h after ingestion of the emulsion in any of the four study protocols.
Plasma glucose, glucose infusion rates, insulin and C-peptide during the 20 mmol/l hyperglycaemic clamp
Insulin secretion rate, insulin sensitivity and insulin clearance
In the present study we demonstrated that, in overweight and obese non-diabetic humans, frequent oral ingestion of fats containing NEFAs with varying degrees of saturation, i.e. MUFA, PUFA or SFA, resulted in differential effects on insulin secretion, action and clearance. Ingestion of PUFA resulted in an absolute reduction in GSIS whereas SFA ingestion induced insulin resistance. Insulin clearance was reduced with ingestion of all fats; however, the reduction was greatest with PUFA. To our knowledge, no previous studies have directly examined the effects of prolonged oral ingestion of fats on GSIS and the action and clearance of insulin in humans.
Previous studies, including our own, that have examined the effect of prolonged elevation of NEFAs on beta cell function and whole-body insulin sensitivity in humans have been performed by infusing a triglyceride emulsion, such as Intralipid, together with a continuous low-dose heparin infusion [13, 15, 20–23]. Because we specifically wished to compare the effect of SFA, MUFA and PUFA on insulin secretion, sensitivity and clearance in the present study and for the other reasons outlined in the Introduction, we turned to a method of oral fat ingestion recently described by Beysen et al. , in which the authors examined the stimulatory effects of an acute elevation of various fatty acids on insulin secretion. This method has not been used previously to examine the effects of a prolonged elevation of NEFA. The protocol used for this study required the patient to ingest the emulsion every hour during the first 12 h and 2-hourly for the second 12 h of the 24 h ingestion period. The disturbed sleep pattern of subjects could have induced a certain degree of stress and it is well known that stress can affect glucose metabolism. Serum cortisol levels were assessed in all subjects at 0 and 24 h, and there were no differences between the four treatment protocols. Since each hourly aliquot of the emulsion contained 136 kcal, the total energy intake during 24 h of oil ingestion for each subject was 2,448 kcal (i.e. 136×18=2,448 kcal), which is slightly above the calculated resting metabolic rates (2,016±100.8 kcal) using the Harris–Benedict equation . Caloric intake during the control study was less than that during the three oil ingestion studies. Because the control study was performed with the subjects ingesting the same volume of hot chocolate-flavoured drink (with water rather than oil added), at the same time intervals as the three oil ingestion studies, the amount of carbohydrate ingested in all studies was sufficient to prevent frank ketosis.
In the present study, PUFA ingestion was the only fat ingestion associated with a significant reduction in absolute GSIS. We have previously shown that infusion for 24–48 h of heparin–Intralipid, which contains mainly PUFA, impairs GSIS in humans [13, 15, 23, 25]. The Intralipid and heparin infusion protocol results in a 4-, 2- and 1.7-fold increase in linoleate, oleate and palmitate levels, respectively, at fasting plasma insulin concentration . In rats, the acute effect of elevated NEFAs in enhancing beta cell function is greatest with SFA, intermediate with MUFA and least with PUFA [10, 27]. In a recent study in humans, short-term elevation of plasma NEFAs by oral fat ingestion augmented GSIS with the order of MUFA>PUFA>SFA . It is less clear whether the prolonged effect of NEFAs in impairing beta cell function also depends on the degree of unsaturation of the fatty acids, and previous studies conducted in a variety of species and using different experimental protocols have shown highly variable results. Data in isolated rat islets  and beta cell lines  indicate that prolonged exposure to oleate (MUFA) might impair GSIS slightly more than exposure to linoleate (n-6 PUFA) or palmitate (SFA). In rats, prolonged i.v. infusion of MUFA had a greater impairing effect on GSIS than n-6 PUFA . Interestingly, in the same rat model, i.v. infusion of SFA did not impair GSIS . In this last study, the effects of a PUFA-rich versus a SFA-rich diet were also compared: the PUFA-rich diet impaired insulin secretion, whereas the diet rich in SFA did not, which is in accordance with the present study using oral fat ingestion in humans. The mechanisms whereby fatty acids differing in saturation might lead to different effects on beta cell function remain unclear. It was suggested that unsaturated but not saturated fatty acids can activate peroxisome proliferator-activated receptor γ (PPARγ) [32–34]. PPARγ upregulates uncoupling protein 2 (UCP2), the overexpression of which impairs GSIS . It is also possible that, compared with saturated fatty acids, unsaturated fatty acids induce a greater degree of oxidative stress, a process that is suggested to underlie glucolipotoxicity [36, 37].
In the present study, SFA ingestion induced insulin resistance. Our results are in line with those of previous studies showing that the impairing effect of high-fat diets on insulin action is proportional to the degree of saturation of fatty acids, SFA inducing the greatest insulin resistance, followed by MUFA and n-6 PUFA, whereas n-3 PUFA may have protective effects [38–40]. We cannot with confidence exclude impairment of insulin sensitivity with the ingestion of PUFA or MUFA, however, because insulin sensitivity was not determined using a gold standard in vivo experimental technique, such as the euglycaemic–hyperinsulinemic clamp.
The fact that only PUFA ingestion resulted in an absolute reduction in insulin secretion in this study may lead one to conclude that only PUFA impairs beta cell function. While we cannot exclude a greater impairing effect of PUFA on beta cell function, it should be considered that with SFA insulin secretion failed to increase to compensate for insulin resistance. In individuals with normal beta cell function, a decline in insulin sensitivity is followed by a compensatory increase in insulin secretion to maintain the ability of the body to dispose of glucose . The fact that ISR was not increased to compensate for the decreased insulin action suggests that there was also impairment in beta cell function in the SFA study, either primary or secondary to the decrease in insulin clearance (see below).
GLP-1 and GIP are two incretins that are secreted by the endocrine L-cells and K-cells, respectively, and potentiate GSIS . MUFA, but not SFA, have been shown to stimulate GLP-1 secretion . Stimulated GLP-1 secretion was not observed in the present study. It is possible that the short half-life of GLP-1 masked the stimulatory effects of ingested fats on GLP-1 secretion. It has also been suggested that fats stimulate GLP-1 secretion only when the total energy intake of an oral fat load exceeds an energy threshold of 400 kcal . In the present study the relatively small amounts of fat ingested in each drink would have been below this threshold, providing one possible explanation for the absence of GLP-1 stimulation. Nevertheless, elevated total plasma GIP suggests the presence of incretin effects on potentiating GSIS.
We have shown previously that elevation of NEFAs induced by intravenous infusion of Intralipid for 24–48 h impairs insulin clearance in humans  and in dogs at both high and low insulin concentrations . In the present study, ingestion of any of the fats decreased insulin clearance, which explains the elevated plasma insulin concentrations in the fat ingestion studies. A reduction in insulin clearance might represent a compensatory mechanism for peripheral insulin resistance or impaired insulin secretion, by providing greater escape of insulin from first-pass hepatic degradation, thereby contributing to peripheral hyperinsulinaemia or preventing peripheral insulin deficiency, respectively . The mechanism for this apparently coordinated reduction in insulin clearance when the action or secretion of insulin is reduced is not known at present. Because reduced insulin clearance can maintain glucose homeostasis in the face of impairment in insulin secretion, it is possible that the reduction in insulin clearance compensates for an impairment of beta cell function. Alternatively, the reduction in beta cell function represents an appropriate beta cell ‘rest’ in response to a reduction in insulin clearance. Although the present study does not answer the question of whether beta cell function is truly impaired by fat ingestion or is simply resting in response to a reduction in insulin clearance, we favour the view that fat ingestion primarily impairs insulin secretion because of the large body of in vitro work from various groups supporting the concept of reduced GSIS in beta cells exposed in a prolonged fashion to high NEFA concentrations  and because of other in vivo studies showing absolute reductions in glucose-stimulated insulin levels after prolonged NEFA elevation [8, 30, 47].
In conclusion, we have demonstrated that continuous oral ingestion of PUFA for 24 h impairs GSIS, that ingestion of SFA reduces whole-body insulin sensitivity, and that ingestion of MUFA, PUFA and SFA all decrease insulin clearance. In the face of insulin resistance in the SFA study, insulin secretion was not increased, which suggests decreased beta cell function in the SFA study, but we cannot exclude greater impairment of beta cell function with PUFA, as evidenced by the absolute reduction in ISR. These data have important implications for understanding the complex interplay between the nature of dietary fat and whole-body insulin, glucose and lipid dynamics, but we acknowledge that long-term dietary intervention studies are required to determine whether the chronic ingestion of a high-fat diet is implicated in the beta cell failure that characterises the conversion from insulin resistance to type 2 diabetes.
This work was supported by an operating grant from the Canadian Diabetes Association. C. Xiao is funded by a New Emerging Team grant from the Canadian Institutes of Health Research. G. F. Lewis holds a Canada Research Chair in Diabetes and is a Career Investigator of the Heart and Stroke Foundation of Canada. A. Carpentier is a new investigator of the Canadian Institutes of Health Research. We would like to thank A. Kuksis (University of Toronto) for performing the fatty acid compositional analyses for this study.