Article

Diabetologia

, Volume 48, Issue 11, pp 2354-2364

First online:

Role of AMP-activated protein kinase in the coordinated expression of genes controlling glucose and lipid metabolism in mouse white skeletal muscle

  • Y. C. LongAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska Institute
  • , B. R. BarnesAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska InstituteDepartment of Physiology and Pharmacology, Section for Integrative Physiology, Karolinska Institute
  • , M. MahlapuuAffiliated withArexis
  • , T. L. SteilerAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska InstituteDepartment of Physiology and Pharmacology, Section for Integrative Physiology, Karolinska Institute
  • , S. MartinssonAffiliated withArexis
  • , Y. LengAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska InstituteShanghai Institute of Materia Medica, Chinese Academy of Sciences
  • , H. Wallberg-HenrikssonAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska InstituteDepartment of Physiology and Pharmacology, Section for Integrative Physiology, Karolinska Institute
  • , L. AnderssonAffiliated withDepartment of Animal Breeding and Genetics, Swedish University of Agricultural SciencesDepartment of Medical Biochemistry and Microbiology, Uppsala Biomedical Centre, Uppsala University
  • , J. R. ZierathAffiliated withDepartment of Surgical Sciences, Section for Integrative Physiology, Karolinska Institute Email author 

Abstract

Aims/hypothesis

AMP-activated protein kinase (AMPK) regulates metabolic adaptations in skeletal muscle. The aim of this study was to investigate whether AMPK modulates the expression of skeletal muscle genes that have been implicated in lipid and glucose metabolism under fed or fasting conditions.

Methods

Two genetically modified animal models were used: AMPK γ3 subunit knockout mice (Prkag3 −/−) and skeletal muscle-specific transgenic mice (Tg-Prkag3 225Q) that express a mutant (R225Q) γ3 subunit. Levels of mRNA transcripts of genes involved in lipid and glucose metabolism in white gastrocnemius muscles of these mice (under fed or 16-h fasting conditions) were assessed by quantitative real-time PCR.

Results

Wild-type mice displayed a coordinated increase in the transcription of skeletal muscle genes encoding proteins involved in lipid/oxidative metabolism (lipoprotein lipase, fatty acid transporter, carnitine palmitoyl transferase-1 and citrate synthase) and glucose metabolism (glycogen synthase and lactate dehydrogenase) in response to fasting. In contrast, these fasting-induced responses were impaired in Prkag3 −/− mice. The transcription of genes involved in lipid and oxidative metabolism was increased in the skeletal muscle of Tg-Prkag3 225Q mice compared with that in wild-type mice. Moreover, the expression of the genes encoding hexokinase II and 6-phosphofrucktokinase was decreased in Tg-Prkag3 225Q mice after fasting.

Conclusions/interpretation

AMPK is involved in the coordinated transcription of genes critical for lipid and glucose metabolism in white glycolytic skeletal muscle.

Keywords

AMP-activated protein kinase Fasting Metabolism Skeletal muscle Transcription