Potential involvement of adipocyte insulin resistance in obesity-associated up-regulation of adipocyte lysophospholipase D/autotaxin expression
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- Boucher, J., Quilliot, D., Pradères, J.P. et al. Diabetologia (2005) 48: 569. doi:10.1007/s00125-004-1660-8
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Autotaxin is a lysophospholipase D that is secreted by adipocytes and whose expression is substantially up-regulated in obese, diabetic db/db mice. The aim of the present study was to depict the physiopathological and cellular mechanisms involved in regulation of adipocyte autotaxin expression.
Autotaxin mRNAs were quantified in adipose tissue from db/db mice (obese and highly diabetic type 2), gold-thioglucose-treated (GTG) mice (highly obese and moderately diabetic type 2), high-fat diet-fed (HFD) mice (obese and moderately diabetic type 2), streptozotocin-treated mice (thin and diabetic type 1), and massively obese humans with glucose intolerance.
When compared to non-obese controls, autotaxin expression in db/db mice was significantly increased, but not in GTG, HFD, or streptozotocin-treated mice. During db/db mice development, up-regulation of autotaxin occurred only 3 weeks after the emergence of hyperinsulinaemia, and simultaneously with the emergence of hyperglycaaemia. Adipocytes from db/db mice exhibited a stronger impairment of insulin-stimulated glucose uptake than non-obese and HFD-induced obese mice. Autotaxin expression was up-regulated by treatment with TNFα (insulin resistance-promoting cytokine), and down-regulated by rosiglitazone treatment (insulin-sensitising compound) in 3T3F442A adipocytes. Finally, adipose tissue autotaxin expression was significantly up-regulated in patients exhibiting both insulin resistance and impaired glucose tolerance.
The present work demonstrates the existence of a db/db-specific up-regulation of adipocyte autotaxin expression, which could be related to the severe type 2 diabetes phenotype and adipocyte insulin resistance, rather than excess adiposity in itself. It also showed that type 2 diabetes in humans is also associated with up-regulation of adipocyte autotaxin expression.
KeywordsAdipocyteAdipose tissueAutotaxindb/dbDiabetesHigh-fat dietHumansMouseObesity
African green monkey
ectonucleotide-pyrophosphate pyrophosphatase phosphodiesterase
Homeostasis model assessment
impaired glucose tolerance, diabetic
normal glucose tolerance
peroxisome proliferator-activated receptor
In parallel to its implication in regulating energy balance via its capacity to store excess lipid energy, adipose tissue is known to possess an important secretory function. It secretes several factors (leptin, adiponectin, TNFα, angiotensinogen, eicosanoids, lysophosphatidic acid, etc.), also called adipokines, that are involved in the development of morbid complications of obesity such as cardiovascular diseases, hypertension, diabetes and cancer .
Our group has described the existence of a new adipokine: autotaxin , which is a soluble protein of 120 Mr belonging to the ectonucleotide-pyrophosphate pyrophosphatase phosphodiesterase (ENPP) family . Autotaxin catalyses a lysophospholipase D-activity [2, 4, 5] leading to the hydrolysis of lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA), a bioactive phospholipid known to control several cellular events via activation of specific G-protein-coupled receptors .
We demonstrated that the expression of autotaxin was substantially up-regulated during adipocyte differentiation in culture, as well as in adipocytes from obese, diabetic db/db mice when compared to non-obese mice . These observations suggested a possible involvement of autotaxin in the normal or pathological development of adipose tissue and/or pathologies associated with obesity.
In the present work, we analysed autotaxin expression in adipose tissue from different mouse models exhibiting various degrees of obesity and/or diabetes, as well as from massively obese human subjects. We concluded that adipocyte autotaxin expression is not directly influenced by excess adiposity, but is rather linked to impaired glucose homeostasis and insulin resistance in adipocytes.
Subjects, materials and methods
Mice were obtained from Charles River Laboratories (l’Arbresle, France) and were handled in accordance with the principles and guidelines established by the National Institute of Medical Research (INSERM). They were housed conventionally in an animal room with constant temperature (20–22°C), humidity (50–60%) and with a 12-h light/dark cycle (lights on at 8.00 h). All mice had free access to food and water throughout the experiments. Obese db/db mice were 5- to 12-week-old C57BlKS/J db/db females fed daily with standard rodent chow. Non-obese controls of db/db mice were C57BlKS/J +/+ mice of the same age and were fed with the same diet. Obese gold thioglucose (GTG) mice were 13-week-old FVB/n females which had received a single intraperitoneal injection (0.5 g/kg) of gold thioglucose (GTG, Sigma, St. Louis, MO, USA) at 8 weeks of age and been fed with a regular diet. Non-obese controls of GTG mice were 13-week-old FVB/n female mice fed with the same diet. Obese high-fat diet (HFD) mice were 13-week-old C57Bl6/J females fed daily with a high-fat diet (energy content distribution in %: 20% protein, 35% carbohydrate and 45% fat) from 5 weeks of age. Non-obese controls of HFD mice were 13-week-old C57Bl6/J mice fed daily with a low-fat diet (energy content distribution in %: 20% protein, 70% carbohydrate and 15% fat). Standardised diets were from UAR, France.
For streptozotocin treatment, 8-week-old C57Bl6/J mice received four daily intra-peritoneal injections of 70 mg/kg streptozotocin and were killed 4 weeks later.
Animals were killed in the non-fasting state. The blood was collected on heparin and glucose was immediately measured with a glucose meter. Plasma concentrations of insulin were determined with an RIA kit (Diagnostics Pasteur, Paris, France). Adipose tissue was dissected out and weighed before separating the adipocytes from the stroma-vascular fraction (see below).
Isolation of adipocytes and stroma-vascular cells of adipose tissue
Adipose tissue was minced and incubated while shaking in 5 ml of DMEM (Gibco, Invitrogen, Paisley, UK) supplemented with 1 mg/ml collagenase and 1% BSA. This was done for 30 min at 37°C. Digested tissue was filtered through a 150-μm screen and centrifuged for 5 min at 900×g in order to get adipocytes in the floating fraction, and stroma-vascular cells (preadipocytes, endothelial cells and macrophages) in the pellet.
Deoxyglucose transport in isolated adipocytes
Deoxyglucose transport was measured as previously described . Isolated adipocytes were incubated in the presence or not of 100 nmol/l insulin for 45 min at 37°C in a final volume of 400 μl. Then, 2-deoxy-d-[3H]-glucose (2-DG, 14.8 kBq) was added at a final concentration of 0.1 mmol/l for 10 min. Assays were stopped with 100 μl of 100 μmol/l cytochalasin B and aliquots of the cell suspension were centrifuged in microtubes containing di-isononyl phthalate (density 0.974 g/ml), to separate adipocytes from the buffer and count the intracellular [3H]2-DG.
Subcutaneous adipose tissue
Human subcutaneous adipose tissue samples were obtained from patients undergoing abdominal dermolipectomy for plastic surgery. No clinical data from these patients were available.
Intra-abdominal adipose tissue
We investigated 23 patients before bariatric surgery. In order to limit any confounding effect, we excluded patients with evidence of chronic or inflammatory diseases. We obtained 20 cm3 of intra-abdominal adipose tissue perioperatively at the level of the omentum. Tissue samples were immediately frozen in liquid nitrogen.
Blood glucose was measured by the glucose oxidase method (Beckman Instruments, Fullerton, CA, USA). Plasma insulin was determined by immunoassay (Insulin IMX, Abbott Laboratories, Tokyo, Japan). The intra-assay coefficient of variation was 4% and the inter-assay coefficient was 6%. Cross reactivity with proinsulin was 0.05%. HDL-cholesterol was measured after precipitation of apo B-containing lipoproteins using sodium phosphotungstate/magnesium chloride (Boehringer Mannheim, Mannheim, Germany). Total cholesterol and triglycerides were measured with commercial kits (Boehringer) adapted to a Hitachi 911 analyser (Japan). LDL-cholesterol was calculated according to Friedewald et al. . Homeostasis model assessment (HOMA) was used to evaluate insulin sensitivity, according to the following formula: HOMA = fasting insulin (mU/ml) × fasting glucose (mmol/l) / 22.5.
HbA1c, determined in patients with known diabetes, was measured as a percentage of the total haemoglobin following high-performance liquid chromatography separation (Diamat, BioRad Laboratories, Hercules, CA, USA) (normal range 4.5–6%).
Some patients were given an OGTT. Subjects, having fasted overnight, received 75 g glucose in water solution, and plasma was sampled before intake and 30, 60, 90, and 120 min afterwards, to determine glucose and insulin.
The subjects of our study gave informed consent and investigations were carried out in accordance with the Declaration of Helsinki as revised in 2000 (http://www.wma.net/e/policy/b3.htm).
We obtained 3T3F442A adipocytes as previously described . Briefly, confluent 3T3F442A preadipocytes were cultured in a differentiating medium consisting of DMEM supplemented with 10% fetal calf serum plus 50 nmol/l insulin. 3T3F442A adipocytes were used after 7 days of culture. African green monkey (COS-7) cells were grown in DMEM supplemented with 10% FCS and transfected with pcDNA3 (Invitrogen) or with pcDNA-m autotaxin-FLAG , using DEAE dextran as previously reported .
RNA extraction and real-time RT-PCR analysis
Total RNAs were extracted from adipose tissue, adipocytes or stroma-vascular cells using the RNeasy mini kit (Qiagen, Hilden, Germany). Total RNA (1 μg) was reverse-transcribed for 60 min at 37°C using Superscript II reverse transcriptase (Life Technology, Invitrogen SARL, Cergy Pontoise, France) in the presence of random hexamers. A minus RT reaction was performed in parallel to ensure the absence of genomic DNA contamination. Real-time PCR was performed starting with 25 ng cDNA and a 900-nmol/l concentration of both sense and antisense oligonucleotides (mouse autotaxin: sense 5′-GACCCTAAAGCCATTATTGCTAA-3′, antisense 5′-GGGAAGGTGCTGTTTCATGT-3′; human autotaxin: sense 5′-GGACCAACATCTCCGGATCTT-3′, antisense 5′-GGAGGTCCAGCCTCTTGAAG-3′) in a final volume of 25 μl using the SYBR green TaqMan Universal PCR Master Mix (Applied Biosystems, Applera, Courtaboeuf, France). Fluorescence was monitored and analysed in a GeneAmp 7000 detection system instrument (Applied Biosystems). Analysis of 18S ribosomal RNA was performed in parallel using the Ribosomal RNA control Taqman Assay Kit (Applied Biosystem) in order to normalise gene expression. Results are expressed as follows: 2(Ct18S–Ctgene) where Ct corresponds to the number of cycles needed to generate a fluorescent signal above a predefined threshold. Oligonucleotide primers were designed using Primer Express software (Perkin-Elmer Life Sciences, Courtaboeuf, France).
Preparation of conditioned media
Adipose tissue explants were prepared from subcutaneous adipose tissue as previously described . Conditioned media were prepared by incubating 7 g of explants in 10 ml of serum-free DMEM for 7 h at 37°C in a humidified atmosphere containing 7% CO2. Conditioned media from 3T3F442A adipocytes and from COS-7-cells were obtained by incubating the cells in 5 ml (for a 10-cm-diameter plate) in serum-free DMEM for 7 h at 37°C in a humidified atmosphere containing 7% CO2. Conditioned media were concentrated (about 50-fold) using an Amicon Ultra 10,000 (Millipore, SAS, St-Quentin en Yvelines, France) and the presence of autotaxin protein was studied by Western blot analysis as well as by measuring its lysophospholipase D activity.
Measurement of lysophospholipase D activity
Lysophospholipase D activity of autotaxin was measured by converting radiolabelled LPC into radiolabelled LPA as previously described . First, a solution of [14C]palmitoyl-lysophosphatidylcholine (NEN 2035 MBq/mmol) at 0.092 kBq/μl in DMEM supplemented with 1% non-esterified fatty acid BSA was prepared, and 20 μl of this solution was incubated with 500 μl thawed conditioned medium plus 1 μl of sodium orthovanadate 0.5 mmol/l for 90 min at 37°C. At the end of the incubation period, phospholipids were extracted with 500 μl of 1-butanol, evaporated, spotted on a silica gel 60 TLC glass plate (Merck International SAS, Strasbourg, France), and separated using CHCl3/MeOH/NH4OH (60:35:8) as a migration solvent. The thin layers were autoradiographed overnight at −80°C using a Biomax-MS film (Kodak) in order to localise radiolabelled LPA spots, which were scraped and counted with 3 ml of scintillation cocktail.
Western blot analysis
We separated 30 μg of protein from concentrated conditioned medium on a Gel Nu-PAGE 4–12% (Invitrogen) and transferred it on to nitrocellulose membrane. The blot was pre-incubated for 1 h at room temperature in TBS/Tween 0.05% containing 3% dried milk, and incubated overnight at 4°C in the same solution supplemented with 0.7 μg/ml autotaxin-antibody. After washing in PBS/Tween 0.05%, autotaxin was visualised by enhanced chemiluminescence detection (ECL, Amersham Biosciences) using an anti rabbit-HRP antibody (Sigma). The autotaxin-antibody (AGRO-BIO, La Ferté Saint Aubin, France) was a rabbit polyclonal antibody directed against the peptide ESCNSSEDESKWVE present in both human (position 881–894) and mouse (position 803–816) autotaxin.
Results are means±SE. Student’s t-test was used to compare two groups of data. Correlations were analysed using the non-parametric Spearman rank test. A p value of less than 0.05 was considered significant.
Adipocyte autotaxin expression is not up-regulated in mouse models of obesity other than db/db mice
Autotaxin is not up-regulated in streptozotocin-induced hyperglycaemic mice
These results show that streptozotocin-induced hyperglycaemia had no influence on adipocyte autotaxin expression, suggesting that hyperglycaemia in itself was not sufficient to explain the up-regulation of adipocyte autotaxin expression in db/db mice.
Association between hyperglycaemia and up-regulation of adipocyte autotaxin expression during the development of db/db mice
These observations reveal that up-regulation of adipocyte autotaxin expression occurred rather late in the onset of obesity in db/db mice, and was more closely associated with the emergence of hyperglycaemia than with fat-store accretion or hyperinsulinaemia.
Up-regulation of autotaxin in adipose tissue of db/db mice is restricted to adipocytes
These observations reveal that the up-regulation of autotaxin expression in adipose tissue of db/db mice was restricted to adipocytes. We concluded that there could be an adipocyte-specific mechanism of regulation of autotaxin gene expression.
Adipocytes from db/db mice exhibit severe impairment of glucose uptake and insulin sensitivity
These results indicate that db/db adipocytes exhibited severe impairment of glucose uptake and insulin sensitivity.
TNFα and rosiglitazone regulate autotaxin expression in 3T3F442A adipocytes
These results indicate that factors altering glucose utilisation and insulin sensitivity in adipocytes were also able to regulate adipocyte autotaxin expression, suggesting a possible involvement of adipocyte insulin sensitivity in adipocyte autotaxin expression.
Expression and secretion of autotaxin expression by human adipose tissue
In contrast to mouse adipose tissue , the expression and secretion of autotaxin by human adipose tissue had not yet been investigated. Secretion and expression of autotaxin were first investigated on fresh subcutaneous abdominal adipose tissue samples obtained from plastic surgery (see “Subjects, materials and methods”). Autotaxin mRNA levels were found to be slightly, but significantly higher in adipocytes than in stroma-vascular cells: autotaxin mRNA/18S RNA 69±13 for adipocytes (n=10); 35±6 for stroma-vascular cells (n=9); p<0.03.
These results show that human adipose tissue expresses and secretes autotaxin.
Expression of autotaxin in adipose tissue from insulin-resistant patients
Autotaxin mRNA levels were quantified in intra-abdominal adipose tissue from 23 women exhibiting massive obesity (mean BMI 48±1) justifying abdominal surgery (gastroplasty) (see “Subjects, materials and methods”). All the patients were hyperinsulinaemic (mean plasma insulin concentration 21.7±2 mU/l, expected normal values 5–6 mU/l) and insulin-resistant (mean HOMA values 5.26±0.58, expected normal values 1–1.2).
Since adipose tissue samples were frozen immediately after surgery, isolation of adipocytes could not be performed and autotaxin expression was analysed on whole-tissue samples from each patient. Autotaxin mRNAs were detected in all samples with autotaxin mRNA/18S RNA ratio values ranging from 12 to 45.
Clinical parameters of obese women either with NGT or with IGT and diabetes
Body weight (kg)
Body mass index (kg/m2)
Plasma glucose (mmol/l)
Plasma insulin (mU/l)
Plasma triglycerides (mmol/l)
Plasma cholesterol (mmol/l)
Plasma HDL (mmol/l)
Plasma LDL (mmol/l)
Oral glucose tolerance test
Glucose after 120 min
These results suggest a possible link between alterations in glucose homeostasis and autotaxin expression in human adipose tissue.
We have previously demonstrated that autotaxin is a lysophospholipase D secreted by adipocytes and is responsible for the synthesis of LPA . We also reported that autotaxin expression is up-regulated in obese-diabetic db/db mice when compared with non-obese mice , suggesting the involvement of adipocyte autotaxin in obesity and/or diabetes.
In the present study, up-regulation of adipocyte autotaxin expression in db/db mice was not observed in other mice models of obesity (GTG, HFD), suggesting the existence of a db/db-specific mechanism involved in regulation of adipocyte autotaxin expression.
Since GTG and HFD mice exhibited equivalent (HFD mice) or higher (GTG mice) adiposity than db/db mice, we conclude that adiposity was not sufficient, in db/db mice, to explain up-regulation of adipocyte autotaxin expression. The same conclusion could be drawn from the analysis of db/db mouse development, showing that young db/db mice (5 weeks of age) already had excess adiposity when compared to control mice, but did not yet exhibit changes in autotaxin expression.
Obese db/db mice had higher hyperinsulinaemia and higher hyperglycaemia than obese GTG or HFD mice. Therefore, db/db mice obviously exhibited a strong impairment of glucose homeostasis. Consequently, db/db-specific up-regulation of adipocyte autotaxin expression could be linked to the type 2 diabetes phenotype of db/db mice. However, analysis of db/db mouse development showed that young (5 weeks) db/db mice already had maximal hyperinsulinaemia, whereas up-regulation of autotaxin expression occurred only at week 8. In addition, in streptozotocin-treated mice, hyperglycaemia reached a level comparable to that observed in db/db mice, but no change in adipocyte autotaxin expression was observed. We can therefore conclude that hyperinsulinaemia or hyperglycaemia in themselves are not sufficient to up-regulate autotaxin expression, suggesting the involvement of an additional regulating factor.
As previously described  and confirmed in the present study, autotaxin was not only expressed in adipocytes, but also in stroma-vascular cells of adipose tissue. However, conversely to adipocytes, autotaxin-expression in stroma-vascular cells from db/db mice was not modified when compared with control mice. This suggests the existence of an adipocyte-specific mechanism involved in the regulation of autotaxin expression in the db/db mouse.
The present work shows that adipocytes from db/db mice exhibited a strong impairment in insulin sensitivity as demonstrated by the reduction in insulin-stimulated glucose uptake when compared with non-obese and HFD-induced obese mice. Since insulin resistance is known to greatly influence gene expression in adipocytes , it is likely that insulin resistance of db/db adipocytes could play a role in the up-regulation of autotaxin gene expression. This hypothesis was supported by the direct positive effect of TNFα (a cytokine that promotes insulin resistance), and the negative regulatory effect of rosiglitazone (a PPARγ agonist well known for its ability to improve insulin sensitivity) on autotaxin expression in 3T3F442A adipocytes.
Finally, the present study shows that autotaxin expression is higher in patients with reduced tolerance to glucose or diabetes mellitus, when compared to patients with NGT. This observation suggests that, as in db/db mice, human autotaxin expression could be influenced by alterations in glucose homeostasis. Human adipose tissue autotaxin expression was not correlated with BMI, plasma glucose, or plasma insulin, indicating that these parameters, as in db/db mice, are not sufficient to explain the up-regulation of autotaxin expression in IGT Diabetes subjects.
Based upon plasma insulin concentrations and HOMA values, there was no apparent difference in insulin sensitivity between NGT and IGT Diabetes subjects. This could indicate that, conversely to db/db mice, insulin resistance was not responsible for regulating autotaxin in human adipose tissue. However, these clinical parameters characterise only global insulin sensitivity and say nothing about insulin sensitivity of adipocytes. Complementary experiments will be necessary to evaluate adipocyte insulin sensitivity in NGT and IGT Diabetes subjects. In parallel, other factors could also be involved in the up-regulation of autotaxin in IGT Diabetes patients.
Indeed, we observed a significant correlation between autotaxin expression and age as well as plasma concentrations of LDL cholesterol. In addition, IGT Diabetes subjects exhibited lower plasma triglyceride and HDL cholesterol. These observations suggest a possible involvement of circulating cholesterol and/or duration of type 2 diabetes in the regulation of autotaxin expression. This hypothesis is supported by a previous report showing an increase of lysophospholipase D-activity in hypercholesterolaemic rabbits .
In conclusion, the present work revealed the existence of a db/db-specific up-regulation of adipocyte autotaxin expression that is independent of excessive adiposity in itself, but could instead be linked to the severe type 2 diabetes phenotype and adipocyte insulin resistance. In parallel, the up-regulation of autotaxin expression in adipose tissue of patients exhibiting type 2 diabetes suggests the possible implication of autotaxin in the human form of the disease.