Seasonality in clinical onset of Type 1 diabetes in Belgian patients above the age of 10 is restricted to HLA-DQ2/DQ8-negative males, which explains the male to female excess in incidence
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- Weets, I., Kaufman, L., Van der Auwera, B. et al. Diabetologia (2004) 47: 614. doi:10.1007/s00125-004-1369-8
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Type 1 diabetes arises from an interplay between environmental and genetic factors. The reported seasonality at diagnosis supports the hypothesis that currently unknown external triggers play a role in the onset of the disease. We investigated whether a seasonal pattern is observed at diagnosis in Belgian Type 1 diabetic patients, and if so whether seasonality varies according to age, sex and genetic risk, all known to affect the incidence of Type 1 diabetes.
The seasonal pattern at clinical diagnosis was assessed in 2176 islet antibody-positive diabetic patients aged 0 to 39 years diagnosed between 1989 and 2000. Additional stratification was performed for age, sex and HLA-DQ genotype.
Overall, a significant seasonal pattern at clinical diagnosis of diabetes was observed (p<0.001). More subjects were diagnosed in the period of November to February (n=829) than during the period of June to September (n=619) characterised by higher averages of maximal daily temperature and daily hours of sunshine. However, the seasonal pattern was restricted to patients diagnosed above the age of 10 (0–9 years: p=0.398; 10–19 years: p<0.001; 20–29 years: p=0.003; 30–39 years: p=0.015). Since older age at diagnosis is associated with a male to female excess and a lower prevalence of the genetic accelerator HLA-DQ2/DQ8, we further stratified the patients aged 10 to 39 years (n=1675) according to HLA-DQ genotype and sex, and we found that the seasonal pattern was largely restricted to male subjects lacking DQ2/DQ8 (n=748; p<0.001 vs all others: n=927; p=0.031).
In a subgroup of male patients diagnosed over the age of 10, the later stages of the subclinical disease process may be more driven by sex- and season-dependent external factors than in younger, female and genetically more susceptible subjects. These factors may explain the male to female excess in diabetes diagnosed in early adulthood.
KeywordsAgeClimateEnvironmental factorsEpidemiologyHLA-DQIncidenceSeasonalitySexType 1 diabetesViral infections
Europe and Diabetes
glutamate decarboxylase antibodies
insulinoma-associated protein 2 antibodies
islet cell cytoplasmic antibodies
Immune-mediated Type 1 diabetes is a multifactorial disease in which destruction of beta cells is triggered by largely unknown environmental or lifestyle factors in genetically susceptible individuals [1, 2]. The role of external factors is illustrated by the incomplete concordance rate (less than 50%) observed in monozygotic twins, by the geographical variation in incidence of Type 1 diabetes, and by the worldwide increase of the disease in young children [2, 3, 4, 5, 6, 7]. Early introduction of cows’ milk proteins, short duration of breastfeeding, rapid growth or weight gain, puberty, pregnancy, obesity, vitamin D deficiency, cold climate, psychological stress, maternal blood group incompatibility, dietary toxins and viral infections have all been proposed to initiate or promote diabetes, but none has emerged so far as the prime aetiological factor [2, 6, 7, 8, 9].
Several epidemiological studies have described a seasonal pattern in the onset of juvenile diabetes, with a peak incidence in winter and a nadir in the warmer summer months [10, 11, 12, 13]. This seasonal variation has been taken as an indirect argument in favour of the role of environmental factors, such as viral infections or cold climate, in the development of the disease. Studies by EURODIAB (Europe and Diabetes) in patients diagnosed under age 15 have shown heterogeneity in the seasonal pattern of clinical onset according to age at diagnosis and region [10, 13]. However, most cases of Type 1 diabetes are diagnosed in people over the age of 15 [1, 14]. Moreover, the disease displays marked age-dependent heterogeneity in terms of incidence rate, inaugural clinical manifestation, biological markers and the proportion of males to females [14, 15, 16]. Indeed, compared with childhood-onset diabetes, adult-onset diabetes has a less severe clinical presentation with a striking male to female excess, in sharp contrast with other autoimmune diseases, and less biological evidence of islet autoimmunity [14, 15, 16]. This includes less prominent insulitis and better preserved beta cell mass , lower prevalences and levels of islet antibodies except for glutamate decarboxylase autoantibodies (GADA), and a lower frequency of the high-risk HLA-DQ2/DQ8 genotype, a genetic accelerator associated with early onset of Type 1 diabetes and with a more rapid progression toward Type 1 diabetes in non-diabetic antibody-positive siblings [18, 19].
Therefore, we investigated whether the seasonal pattern of clinical onset of Type 1 diabetes is a general characteristic of immune-mediated diabetes or whether it is restricted to subgroups with older age at diagnosis, subgroups of males and/or subgroups with fewer genetic accelerators. The prospective registration of clinical and demographic data from Type 1 diabetic patients diagnosed under age 40 in Belgium, and the assessment of their initial biological characteristics (islet autoantibodies, HLA-DQ genotype) during a 12-year period of activity allowed us to address this issue.
Subjects and methods
Between 1 January 1989 and 31 December 2000, 3271 diabetic patients were prospectively registered by the Belgian Diabetes Registry through voluntary reporting by participating diabetologists. The patients were all diagnosed according to National Diabetes Data Group criteria , were aged between 0 and 39 years, and were resident in Belgium. Of the 3271 registered patients, 2176 (1242 male and 934 female) were finally included after verification of the following criteria (some patients had more than one exclusion criterion): (i) having primary diabetes (cases of gestational diabetes, secondary diabetes or diabetes of unknown type were excluded, n=102); (ii) being of Caucasian descent (European or northern African) (excluded: n=132); (iii) being resident in Belgium for at least 6 months prior to diagnosis (excluded: n=0); (iv) having available demographic data, and available blood samples, taken within 18 months of diagnosis, for DNA and auto-antibody analysis (excluded: n=523); (v) testing positive for at least one type of diabetes-associated autoantibody (including islet cell cytoplasm antibodies [ICA], insulin autoantibodies [IAA], insulinoma-associated protein 2 antibodies [IA-2A] and GADA; excluded, n=414) to establish the presence of immune-mediated Type 1 diabetes . Comparison with a sub-region (Antwerp district) with near-complete case ascertainment (94%) has demonstrated that the larger Belgian group of patients, with incomplete ascertainment (52% over the entire study period), is representative for the Belgian diabetic patients who were diagnosed below age 40 [5, 22].
We also stratified the sample group according to the presence or absence of HLA-DQ susceptibility genotypes. DNA was extracted from K-EDTA blood, amplified and HLA-DQA1*-DQB1* genotyped as described previously . Based on the comparison of HLA-DQA1-DQB1 genotypes in 1866 antibody-positive recent-onset diabetic patients aged under 40 years at diagnosis (recruited in Belgium between January 1989 and March 1999) and in 750 non-diabetic Belgian residents, the following genotypes were found to confer significant susceptibility to Type 1 diabetes in the Belgian population: (i) DQA1*0501-DQB1*0201/DQA1*0301-DQB1*0302 (DQ2/DQ8); (ii) homozygosity for DQA1*0301-DQB1*0302 (DQ8) or DQA1*0501-DQB1*0201 (DQ2); and (iii) DQA1*0301-DQB1*0302 (DQ8) in combination with one of the neutral DQA1*-DQB1* haplotypes (0100–0501/0604/0605, 0102–0201, 0102–0502, 0301–0201, 0301–0301, 0301–0303, 0301–0401, 0401–0402 or 0501–0302) . In the present study, patients aged 10 to 39 years at diagnosis (n=1675) were further stratified according to the presence (n=418) or absence (n=1257) of the highest risk HLA-DQ2/DQ8 genotype preferentially associated with young age at diagnosis . Furthermore, additional stratification was carried out according to the presence of one of the other susceptible genotypes (DQ2/DQ2, DQ8/DQ8 or DQ8 in combination with a neutral genotype, not preferentially associated with young age at diagnosis; n=538) or the presence of one of the protective, rare or neutral genotypes (n=719) as described previously .
For the duration of the study, monthly averages for daily maximal temperature and daily hours of sunshine in central Belgium (Zaventem) were obtained from the Royal Belgian Meteorological Institute (Brussels, Belgium).
The study was approved by the ethics committees of the Belgian Diabetes Registry and of the universities participating in its scientific projects. Informed consent was obtained from each subject and/or their parents in accordance with the Helsinki Declaration.
Immune and genetic markers
We measured ICA by indirect immunofluorescence assay using cryosections of freshly frozen human blood group O pancreas for substrate . The results were expressed in Juvenile Diabetes Foundation units. We detected IAA, GADA and IA-2A by liquid-phase radiobinding assay using, respectively, recombinant human 125I-labelled insulin, 35S-labelled intact GAD (65 Mr isoform) and the 35S-labelled intracellular domain of IA-2 (IA-2ic) for tracer . The cDNA of human GAD65 was kindly provided by Professor Å. Lernmark (University of Washington, Seattle, Wash., USA) and the IA-2ic by Dr M. Christie (King’s College, London, UK). The recombinant human insulin used for preparing insulin tracer was provided by Dr Schmidt (Humulin; Eli Lilly, Brussels, Belgium). Cut-off values for positivity for ICA (≥12 Juvenile Diabetes Foundation units), IAA (≥0.6% tracer bound), GADA (≥2.6% tracer bound) and IA-2A (≥0.4% tracer bound) were established to secure 99% diagnostic specificity after omission of outlying values based on the analysis of 789 healthy control subjects . The antibody assays performed repeatedly well in successive external quality control programmes (Immunology of Diabetes Workshops , proficiency testing of the University of Florida [Gainesville, Fla., USA] and of the Research Institute for Children [New Orleans, La., USA], and the Diabetes Antibody Standardization Program ). In the Immunology of Diabetes combinatorial islet autoantibody workshop, diagnostic sensitivity adjusted for 99% specificity amounted to 73% for ICA, 85% for GADA and 36% for IAA (IA-2A was not yet available in our laboratory at the time of the workshop ). In the Diabetes Antibody Standardization Program in 2002, sensitivity reported at 90% specificity was 64% for IAA, 90% for GADA and 68% for IA-2A.
To evaluate seasonality in diagnosis of diabetes, the statistical significance of any deviation of the observed number of subjects from the expected number of subjects was calculated using the Roger’s test . This method assumes a sinusoidal pattern over the months of the year with a peak and a nadir that are 6 months apart, and provides a χ2 statistic on 2 degrees of freedom. The parameters describing the model (α and β) were determined by non-linear regression analysis. Data were also analysed after stratification for age at onset, sex and HLA-DQ genotypes or haplotypes. We adjusted for variability of month length by transforming the observations into results for months of equal length (365/12 days). The amplitude of oscillation (expressed as a percentage) was calculated from the mean of the highest and lowest expected number of subjects, which was determined by the model. Statistical tests were two-tailed and were performed using Microsoft Excel 2000. We considered p values of less than 0.05 to be statistically significant.
Because older age at diagnosis is associated with a striking male predominance and a lower prevalence of the HLA-DQ2/DQ8 risk genotype [14, 15, 16, 18], all patients under age 10 at diagnosis were compared with an equal number of patients aged between 10 and 40 years after matching for sex and HLA-DQ2/DQ8. The observed difference in seasonality persisted after matching for both confounders (0–9 years: n=364, χ2=3.0, p=0.221; 10–39 years: n=364, χ2=8.0, p=0.018; not shown). Seasonality was further studied in the patient group aged 10 to 39 years, which had a significant seasonal pattern at clinical diagnosis, after stratification according to sex and HLA-DQ status. There was a significant seasonal pattern in male (n=983; χ2=37.8, p<0.001) but not in female (n=692; χ2=4.9, p=0.086) patients. This sex-dependent difference persisted when all female patients aged 0 to 39 years were matched for age and DQ2/DQ8 to an equal number of male patients (males: n=934, χ2=20.4, p<0.001; females: n=934, χ2=5.4, p=0.069; not shown). Due to the preferential occurrence of a seasonal pattern at diagnosis in male patients, the male to female excess is mainly restricted to the high incidence period (November–February: 1.66; June–September: 1.06; p=0.001). In addition, regardless of sex, seasonality in those diagnosed over the age of 10 was significant in the absence of the DQ2/DQ8 genotype (n=1257; χ2=27.2, p<0.001) but only borderline in carriers of DQ2/DQ8 (n=418; χ2=7.5, p=0.024; not shown). Furthermore, we compared the seasonal pattern in patients carrying susceptible HLA-DQ genotypes other than DQ2/DQ8 (as identified in the Belgian population)  with that of patients with a protective, rare or neutral genotype (see Methods for genotype classification) . In both groups a significant seasonality was found (susceptible genotypes other than DQ2/DQ8: n=538, χ2=15.8, p<0.001; protective/rare/neutral HLA-DQ genotypes: n=719, χ2=14.1, p<0.001; not shown).
Effects of age, sex and HLA-DQ genotype on seasonal pattern at diagnosis in immune-mediated diabetes
Consistent with previous reports [10, 11, 12, 13], we found a significant seasonal pattern at clinical diagnosis of immune-mediated diabetes in Belgium, with peak values between November and February. In contrast to previous reports focusing on diabetes diagnosed in patients under age 15, the present study has also investigated patients diagnosed between 15 and 40 years of age. In order to avoid variable admixture of Type 2 or idiopathic Type 1 diabetes with uncertain aetiology, all patients were selected on the basis of antibody positivity as proof of the presence of an immune-mediated disease process. Consistent with observations from EURODIAB [10, 13], the seasonal pattern was comparable with a sinusoidal curve, and the observed amplitude of oscillation averaged ±17.2%. At variance with EURODIAB, where age groups 0 to 4, 5 to 9 and 10 to 14 were compared, seasonality was only significant above age 10. This discrepancy is possibly due to a lower number of observations under age 10 in Belgium compared with in the European study, but may also be due to regional differences as described in the EURODIAB studies . Overall, our results confirm and extend other reports that seasonality of Type 1 diabetes onset increases with age at diagnosis [10, 13, 27].
Compared with juvenile Type 1 diabetes, adult-onset disease (≥15 years) is characterised by a striking male predominance and a lower frequency of the high-risk HLA-DQ2/DQ8 genotype. This genotype is the strongest known genetic accelerator associated with early onset of Type 1 diabetes and with more rapid progression toward Type 1 diabetes in non-diabetic antibody-positive siblings [1, 18, 19]. We therefore investigated whether the apparent age dependency of seasonality could be due to differences in seasonal pattern in terms of sex- and/or HLA-DQ-linked genetic predisposition rather than due to differences in age at diagnosis. Our results indicate that seasonality at diagnosis is not a general characteristic of immune-mediated diabetes but seems largely restricted to older male patients lacking the highest risk genotype HLA-DQ2/DQ8. The data suggest that in a subgroup of male pre-diabetic subjects, the underlying pathological process is driven to a greater extent by environmental or lifestyle factors than in other patients, as further supported by (i) the observation of an equally large male to female excess in age-matched adult-onset Type 2 patients ; (ii) the association between GADA positivity and being overweight in male but not in female non-diabetic first degree relatives of Type 1 diabetic patients ; (iii) the secular trend toward decreasing age at diagnosis in male but not in female patients in Belgium ; and (iv) the existence of a seasonal pattern at diagnosis in antibody-negative patients lacking DQ2/DQ8 in this study. Our observations are also compatible with results from EURODIAB describing a less-pronounced seasonality in Scandinavian regions with a high incidence of childhood-onset diabetes and a high prevalence of susceptible HLA-DQ genotypes [10, 13, 28].
Putative external factors contributing to the seasonal pattern in males
It has been proposed that several external aetiological factors influence the incidence of Type 1 diabetes. These include viral infections, maternal blood group incompatibility, dietary toxins, early introduction of cows’ milk, avoidance of breastfeeding, vitamin D deficiency, obesity, cold climate, puberty, pregnancy, rapid growth or weight gain, and psychological stress [6, 8, 15, 29, 30, 31, 32]. The diversity of these factors raises the question of whether they act through a limited number of common pathways. Since the destructive insulitis of Type 1 diabetes is believed to result from changes in the beta cells and/or the immune system, aetiological external factors are likely to directly or indirectly alter at least one of these cell systems [17, 33]. The male to female excess in Type 1 diabetes contrasts sharply with the striking female preponderance in other autoimmune diseases . As there is growing evidence that an increased metabolic burden drives the disease process underlying immune-mediated diabetes [7, 35, 36], and since there is also a large male bias in Type 2 diabetes diagnosed in early adulthood , it is tempting to speculate that the metabolic component of Type 1 diabetes may play an important role in the bias. Most of the observed risk factors (e.g. a wide range of viral infections, and perinatal stressful events such as maternal blood group incompatibility, psychological stress, rapid growth, obesity, puberty, pregnancy, cold climate) may have as a common denominator that they increase the metabolic strain on the beta cells [29, 36, 37]. Other factors, such as vitamin D deficiency, may act preferentially on the immune system .
The restriction of the male excess to the winter months suggests that one or several aetiological factors display seasonal variation, and that they are more persistent in men owing to their higher susceptibility or owing to a higher level of protection in women. In view of the observed north–south gradient in the incidence of Type 1 diabetes throughout Europe, and the worldwide observation of an incidence peak during the winter months, less daylight and low ambient temperature as well as their metabolic concomitants, e.g. higher vitamin D requirements, higher food intake or less exercise, may play a precipitating role [6, 7, 31, 32]. In line with this assumption, we found that the monthly averages of maximal daily temperature and daily hours of sunshine were inversely related to the number of new patients per month in Belgium. Interestingly, the incidence of diabetes in non-obese diabetic mice was lower when animals were kept at a higher ambient temperature . It is conceivable that male subjects, with their overall greater height and weight and/or more central fat distribution, are selectively sensitive to the additional metabolic burden caused by many of the external risk factors. Seasonal variation in testosterone levels and waist-to-hip ratio was noted in Norwegian men, with peak values in December and a nadir in August . Moreover, unlike androgens, oestrogens either alone or in combination with progestins may have a beneficial effect on blood glucose levels in women . Viral infections, vitamin D requirements and psychological stress are also likely to present a seasonal pattern.
In conclusion, the presence of a seasonal pattern at diagnosis of Type 1 diabetes is largely restricted to male patients lacking the HLA-DQ2/DQ8 high-risk genotype. Sex-related differences in incidence, age distribution and seasonality of Type 1 diabetes may be due to a variable contribution of genetic and external aetiological factors in the disease process in terms of age, sex and season. Future studies that aim to identify environmental factors may benefit from focusing on patient subgroups with marked seasonality at diagnosis, e.g. male subjects over the age of 10 lacking DQ2/DQ8. In risk groups such as first-degree relatives, these subjects may also become the best targets for primary prevention of Type 1 diabetes.
The present work was supported by the Fund for Scientific Research (Flanders, Belgium; grants 3-0113-97, 3-0456-99 and G-0319-01, and Levenslijn-Diabetes project 7.0021.96) and by Bayer (Leverkusen, Germany), Life Scan (Beerse, Belgium), Novo Nordisk (Bagsvaerd, Denmark), Ortho Clinical Diagnostics(Beerse, Belgium) and Roche (Vilvoorde, Belgium). The central unit of the Belgian Diabetes Registry is supported financially by grants from the Ministries of Public Health of the Flemish and French Community. We thank the Flemish Diabetes Association, the Belgian Diabetes Association (French-speaking branch) and the following members of the Belgian Diabetes Registry for their invaluable help in recruiting diabetic patients and first-degree relatives and/or in the handling of blood samples: P. Abrams, E. Anckaert, P. Arnouts, E. Balasse, D. Ballaux, J.-P. Baeyens, D. Beckers, H. Becq, A. Beirinckx, J. Beirinckx, M. Bex, A. Bocquet, A. Bodson, R. Bouillon, J. Bourguignon, H. Bossaert, M. Bouquegneau, H. Brussaard, M. Buysschaert, M. Cardon, A. Carlier, K. Casteels, E. Cavatorta, A. Chachati, C. Claeys, L. Claeys, M. Claeys, M. Cnop, P. Cochez, M. Coeckelberghs, I.M. Colin, J.-L. Coolens, P. Coremans, B. Corvilain, P. Couck, F. Coucke, W. Coucke, E. Couturier, R. Craen, A. Crapé, L. Crenier, P. Damoiseaux, C. Daubresse, J.-C. Daubresse, C. De Block, K. Decochez, P. Decraene, I. De Feyter, F. Defoer, R. De Hauwere, I. De Leeuw, K. De Keyser, B. Delgrange, R. Demaeseneer, L. Derdelinckx, J. De Schepper, D. Désir, G. De Wasch, S. Deweer, L. Dooms, H. Dorchy, M. Du Caju, J. Ducobu, E. Duvivier, F. Duyck, A. Dysseleer, L. Emsens, C. Ernould, C. Evenepoel, A. Eykens, A. Fassotte, F. Féry, R. Fils, N. Gaham, K. Garmijn, J. Gérard, I. Geronooz, P. Gillard, C. Gillet, M. Giri, F. Gorus, J. Guiot, A. Haemers, S. Haemers, F. Hay, C. Hendrieckx, C. Herbaut, G. Heremans, F. Heureux, G. Hubermont, J. Huyghe, V. Immegeers, B. Jandrain, P. Jopart, B. Keymeulen, Y. Kockaerts, G. Krzentowski, K. Laga, E. Lagasse, G. Lamberigts, M.-C Lebrethon, P. Lefèbvre, M. Letiexhe, B. Leus, F. Lienart, T-T. Lim, C. Litvine, K. Logghe, M. Maes, W. Maes, M. Marchal, P. Mariën, C. Mathieu, Y. Maus, J. Mockel, J. Monballyu, G. Moorkens, E. Muls, W. Musch, P. Nachtegaele, D. Nicolaij, F. Nobels, A. Nollet, F. Paciorkowski, N. Paquot, F. Peiffer, M.-C. Pelckmans, H. Penninckx, O. Peters, J.-C. Philips, M. Pieron, D. Pipeleers, L. Poelmans, A. Purnode, C. Remy, C. Righes, D. Rocour-Brumioul, R. Rooman, R. Rottiers, D. Scarniere, A. Scheen, E. Schils, I. Schoemaker, M. Schueman, F. Schuit, J. Schutyser, N. Seret, J.-C. Sodoyez†, G. Somers†, M.-P. Stassen, M. Struelens, P. Taelman, L. Terriere, J. Teuwen, G. Thenaers, G. Thiry-Counson, J. Tits, I. Truyen, J. Unger, K. Van Acker, E. Van Aken, P. Van Belle, P. Van Crombrugge, H. Van De Cauter, E. Vandemeulebroucke, M. Vandenbroucke, A. Van Den Bruel, E. Vandenbussche, B. Van der Auwera, M. Vanderijst, H. Vanderstappen, E. Vanderstraeten, D. Van Doorn, E. Van Fleteren, L. Van Gaal, L. Van Garsse, E. Van Gastel, D. Van Gijsegem, M. Van Helvoirt, S. Van Imschoot, S. Vanneste, D. Vannimmen, C. Van Parijs, P. Van Rooy, C. Van Winghem, R. Verbiest, C. Vercammen, A. Verhaegen, G. Verhaert, I. Verschraegen, J. Verschuere, E. Weber, I. Weemaes, G. Wyffels.
We are indebted to N. Alaerts, V. Baeten, M. Bodson, M. Carpentier, A. Ceusters, V. Claessens, N. Christophe, E. De Man, A. Demarré, T. Demesmaeker, S. De Neve, L. De Pree, S. Exterbille, T. Ghysels, P. Goubert, C. Groven, A. Hutse, A. Ivens, D. Kesler, C. Lauwers, F. Lebleu, J. Michiels, H. Morobé, E. Quartier, G. Schoonjans, H. Thomas and J. Vertommen for excellent technical assistance.