Article

Diabetologia

, Volume 47, Issue 5, pp 816-820

Adiponectin receptors gene expression and insulin sensitivity in non-diabetic Mexican Americans with or without a family history of Type 2 diabetes

  • A. E. CivitareseAffiliated withPennington Biomedical Research Center
  • , C. P. JenkinsonAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , D. RichardsonAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , M. BajajAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , K. CusiAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , S. KashyapAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , R. BerriaAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , R. BelfortAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
  • , R. A. DeFronzoAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
    • , L. J. MandarinoAffiliated withDivision of Diabetes, Department of Medicine, University of Texas Health Science Center
    • , E. RavussinAffiliated withPennington Biomedical Research Center Email author 

Abstract

Aims/hypothesis

The recent discovery of two adiponectin receptors (AdipoR1 and AdipoR2) will improve our understanding of the molecular mechanisms underlying the insulin-sensitising effect of adiponectin. The aim of this study was to determine for the first time whether skeletal muscle AdipoR1 and/or AdipoR2 gene expression levels are associated with insulin resistance.

Methods

Using RT-PCR and northern analysis we measured AdipoR1 and AdipoR2 gene expression in skeletal muscle from healthy Mexican Americans with normal glucose tolerance who had (n=8) or did not have (n=10) a family history of Type 2 diabetes.

Results

Gene expression profiling indicated that the AdipoR1 and AdipoR2 isoforms are highly expressed in human skeletal muscle, unlike in mice where AdipoR2 expression was highest in the liver, and AdipoR1 was highest in skeletal muscle. In the study subjects, the expression levels of AdipoR1 (p=0.004) and AdipoR2 (p=0.04), as well as plasma adiponectin concentration (p=0.03) were lower in people with a family history of Type 2 diabetes than in those with no family history of the disease. Importantly, the expression levels of both receptors correlated positively with insulin sensitivity (r=0.64, p=0.004 and r=0.47, p=0.048 respectively).

Conclusions/interpretation

Collectively, these data indicate that both isoforms of the adiponectin receptor play a role in the insulin-sensitising effect of adiponectin.

Keywords

Adiponectin AdipoR1 AdipoR2 Insulin resistance Mexican American Type 2 diabetes