Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension
- F. PalmAffiliated withDepartment of Medical Cell Biology, Biomedical CenterDepartment of Diagnostic Radiology, Uppsala University Email author
- , J. CederbergAffiliated withDepartment of Medical Cell Biology, Biomedical Center
- , P. HansellAffiliated withDepartment of Medical Cell Biology, Biomedical Center
- , P. LissAffiliated withDepartment of Medical Cell Biology, Biomedical CenterDepartment of Diagnostic Radiology, Uppsala University
- , P.-O. CarlssonAffiliated withDepartment of Medical Cell Biology, Biomedical CenterDepartment of Medical Sciences, Uppsala University
Augmented formation of reactive oxygen species (ROS) induced by hyperglycaemia has been suggested to contribute to the development of diabetic nephropathy. This study was designed to evaluate the influence of streptozotocin (STZ)-induced diabetes mellitus, as well as the effects of preventing excessive ROS formation by α-tocopherol treatment, on regional renal blood flow, oxygen tension and oxygen consumption in anaesthetized Wistar Furth rats.
Non-diabetic and STZ-diabetic rats were investigated after 4 weeks with or without dietary treatment with the radical scavenger DL-α-tocopherol (vitamin E, 5%). A laser-Doppler technique was used to measure regional renal blood flow, whilst oxygen tension and consumption were measured using Clark-type microelectrodes.
Renal oxygen tension, but not renal blood flow, was lower throughout the renal parenchyma of diabetic rats when compared to non-diabetic control rats. The decrease in oxygen tension was most pronounced in the renal medulla. Renal cellular oxygen consumption was markedly increased in diabetic rats, predominantly in the medullary region. Diabetes increased lipid peroxidation and protein carbonylation in the renal medulla. Treatment with α-tocopherol throughout the course of diabetes prevented diabetes-induced disturbances in oxidative stress, oxygen tension and consumption. The diabetic animals had a renal hypertrophy and a glomerular hyperfiltration, which were unaffected by α-tocopherol treatment.
We conclude that oxidative stress occurs in kidneys of diabetic rats predominantly in the medullary region and relates to augmented oxygen consumption and impaired oxygen tension in the tissue.
KeywordsNephropathy oxygen tension reactive oxygen species vitamin E lipid peroxidation Type 2 diabetes
- Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension
Volume 46, Issue 8 , pp 1153-1160
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- oxygen tension
- reactive oxygen species
- vitamin E
- lipid peroxidation
- Type 2 diabetes
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- Author Affiliations
- 1. Department of Medical Cell Biology, Biomedical Center, Box 571, 751 23, Uppsala, Sweden
- 2. Department of Diagnostic Radiology, Uppsala University, Uppsala, Sweden
- 3. Department of Medical Sciences, Uppsala University, Uppsala, Sweden