Diabetologia

, Volume 45, Issue 2, pp 276–282

Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells

  • J. Hoffstedt
  • M. Rydén
  • P. Löfgren
  • M. Orho-Melander
  • L. Groop
  • P. Arner
ARTICLE

DOI: 10.1007/s00125-001-0732-2

Cite this article as:
Hoffstedt, J., Rydén, M., Löfgren, P. et al. Diabetologia (2002) 45: 276. doi:10.1007/s00125-001-0732-2

Abstract

Aims/hypothesis

A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type II (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells.

Methods

Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects.

Results

For UCSNP-43 the G/G genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/μg RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects.

Conclusions/interpretation

The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism. [Diabetologia (2002) 45: 276–282]

Keywords Adipose tissueGLUT-4insulinlipogenesislipolysismRNApolymerase chain reaction
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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • J. Hoffstedt
    • 1
  • M. Rydén
    • 1
  • P. Löfgren
    • 1
  • M. Orho-Melander
    • 2
  • L. Groop
    • 2
  • P. Arner
    • 1
  1. 1.Department of Medicine, Huddinge University Hospital (CME), Stockholm, SwedenSweden
  2. 2.Department of Endocrinology, University Hospital, Lund University, Malmö, SwedenSweden