Theoretical and Applied Genetics

, Volume 103, Issue 2, pp 288–296

Inheritance of fenoxaprop-P-ethyl resistance in a blackgrass (Alopecurus myosuroides Huds.) population

  • A. Letouzé
  • J. Gasquez

DOI: 10.1007/s001220100607

Cite this article as:
Letouzé, A. & Gasquez, J. Theor Appl Genet (2001) 103: 288. doi:10.1007/s001220100607

Abstract 

A blackgrass population has developed resistance to fenoxaprop-P-ethyl following field selection with the herbicide for 6 consecutive years. Within this population, 95% of the individuals are also resistant to flupyrsulfuron. Both the inheritance(s) and the mechanism(s) of resistances were investigated by making crosses between the resistant and a susceptible biotype. The inheritance was followed through the F1 and F2 generations either by spraying the herbicide on seedlings at the three-leaf stage or using a seedling bioassay, based on coleoptile length. No maternal effects were evident in the fenoxaprop-P-ethyl responses of the F1 plants, suggesting that the inheritance was nuclear. Some F1 families treated with fenoxaprop-P-ethyl segregated in a 3:1 (resistant:susceptible) ratio, indicating that the resistance was conferred by two dominant and independent nuclear genes. This was confirmed by the 15:1 (R:S) ratio observed in the F2 generation treated with fenoxaprop- P-ethyl. The use of selective inhibitors of herbicide de-toxifying enzymes (aminobenzotriazole, pyperonylbutoxide, malathion and tridiphane) with the F2 plants suggested that each of the two genes may govern two different mechanisms of fenoxaprop-P-ethyl resistance: the ACCase mutation previously postulated and an enhanced herbicide metabolism, mediated by cytochrome P 450 mono-oxygenases (P 450) susceptible to malathion. The P 450 activity may also confer resistance to flupyrsulfuron. This study clearly indicates that two distinct mechanisms of resistance may co-exist in the same plant.

Keywords Fenoxaprop-P-ethyl Flupyrsulfuron Herbicide resistance Blackgrass Inheritance 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • A. Letouzé
    • 1
  • J. Gasquez
    • 1
  1. 1.Institut National de la Recherche Agronomique, Unité de Malherbologie et Agronomie, BP 86510, 21065 Dijon cedex, France, Present address A. Letouzé, Institut Universitaire Technologique, Département ”Génie Biologique”, 39 rue Paul Mazy, 24019 Périgueux cedex, FranceFR

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