Theoretical and Applied Genetics

, Volume 103, Issue 2, pp 197–203

Differentiation and inter-genomic relationships among C, E and D genomes in the Oryzaofficinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization

  • C.-B. Li
  • D.-M. Zhang
  • S. Ge
  • B.-R. Lu
  • D.-Y. Hong
Original Paper

DOI: 10.1007/s001220100562

Cite this article as:
Li, CB., Zhang, DM., Ge, S. et al. Theor Appl Genet (2001) 103: 197. doi:10.1007/s001220100562

Abstract 

The multicolor genomic in situ hybridization (McGISH) method was used to study differentiation and relationships among the C, D and E genomes in the officinalis complex of the genus Oryza. The chromosomes of Oryza alta (CCDD genomes) were hybridized with labelled probes of the C genome (from diploid Oryza eichingeri and Oryza officinalis) and the E genome (from Oryza australiensis) simultaneously. By adjusting the post-hybri- dization washing stringency in a gradual series, differentiation between the genomes was detected according to the homology between the target genomes and the probes. The McGISH results indicate that the C, D and E genomes share a substantial amount of similar sequences, and differentiation between the D and C genomes of O. alta is less than that between the E genome and each of the C and D genomes. The differentiation within the C genomes of the diploid species (O. officinalis and O. eichingeri) and the C genome of O. alta was clearly discerned by McGISH, suggesting strongly that neither O. officinalis nor O. eichingeri was the direct C-genome donor of O. alta. The evidence of the GISH results also indicates that the E genome was considerably differentiated from the C and D genomes. Therefore, the E genome should not be the direct donor of O. alta; on the contrary, the E genome is closer to the C than to the D genome. McGISH is an efficient method in revealing the relationships among the genomes in question, particularly under the gradual stringent-washing condition.

Keywords Genomic in situhybridization (GISH) Oryza officinalis complex C genome E genome CD genome 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • C.-B. Li
    • 1
  • D.-M. Zhang
    • 1
  • S. Ge
    • 1
  • B.-R. Lu
    • 2
  • D.-Y. Hong
    • 1
  1. 1.Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China e-mail: zhangdm@ns.ibcas.ac.cnCN
  2. 2.Genetic Resources Center, International Rice Research Institute (IRRI), MCPO Box 3127, 1271 Makati City, The Philippines, Current address: Institute of Biodiversity Science, Fudan University, Shanghai 200433, ChinaCN