Theoretical and Applied Genetics

, Volume 102, Issue 5, pp 726–732

Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat

Authors

  • J. S. S. Ammiraju
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
  • B. B. Dholakia
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
  • D. K. Santra
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
  • H. Singh
    • Biotechnology Centre, Punjab Agricultural University, Ludhiana, India
  • M. D. Lagu
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
  • S. A. Tamhankar
    • Agharkar Research Institute, Pune, India
  • H. S. Dhaliwal
    • Biotechnology Centre, Punjab Agricultural University, Ludhiana, India
  • V. S. Rao
    • Agharkar Research Institute, Pune, India
  • V. S. Gupta
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
  • P. K. Ranjekar
    • Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India e-mail: pkr@ems.ncl.res.in Fax: +91-20 5884032
Original Article

DOI: 10.1007/s001220051703

Cite this article as:
Ammiraju, J., Dholakia, B., Santra, D. et al. Theor Appl Genet (2001) 102: 726. doi:10.1007/s001220051703

Abstract 

The feasibility of identifying inter-simple sequence repeat markers associated with seed weight in hexaploid wheat was tested using 113 recombinant inbred lines developed by the single-seed descent method, from a cross between Rye selection111, an Indian genetic stock obtained through the introgression of genes for bold seed size from rye, and Chinese Spring having small seed size. Three markers were associated with low seed size with gene effects of 14.8%, 9.5%, and 6%, while four markers with contributions of 8%, 4.66%, 2.92% and 2.61% were found to be linked to high seed size, together contributing 31% of the phenotypic variance in seed size. Nulli-tetrasomic and di-telosomic analysis revealed the presence of three low seed size QTL-associated markers on three chromosomes, 6BL, 2DL, and 1DS respectively. This study clearly demonstrates that ISSRs are highly useful for finding markers associated with major and minor genes controlling agronomically important traits in wheat.

Keywords WheatThousand kernel weight (TKW)Quantitative trait loci (QTLs)Inter simple sequence repeat (ISSR) markersRecombinant inbred lines (RILs)Nullitetrasomic (NT) lines
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© Springer-Verlag Berlin Heidelberg 2001