Theoretical and Applied Genetics

, Volume 102, Issue 5, pp 664–675

Genetic mapping of expressed sequence tag polymorphism (ESTP) markers in loblolly pine (Pinus taeda L.)

  • B. Temesgen
  • G. R. Brown
  • D. E. Harry
  • C. S. Kinlaw
  • M. M. Sewell
  • D. B. Neale
Original Paper

DOI: 10.1007/s001220051695

Cite this article as:
Temesgen, B., Brown, G., Harry, D. et al. Theor Appl Genet (2001) 102: 664. doi:10.1007/s001220051695

Abstract 

The development and mapping of genetic markers based upon expressed sequence tag polymorphisms (ESTPs) in loblolly pine (Pinus taeda L.) are reported. The new markers were generated by PCR-amplification of loblolly pine genomic DNAs with primers designed from sequenced cDNAs. The cDNA libraries were constructed from RNAs expressed in the needles of loblolly pine seedlings or in the xylem from young trees. DNA polymorphisms were identified by analyzing the amplified products for differences in fragment size or restriction sites, or by examining mobility differences using denaturing gradient gel electrophoresis (DGGE). DGGE revealed more DNA polymorphisms than the other two methods. Fifty six ESTPs were mapped using either of two mapping populations and positioned onto a loblolly pine consensus genetic map. Unlike many other markers commonly used in forestry, ESTPs can be used as orthologous markers for comparative mapping, to map genes of known function, or to identify candidate genes affecting important traits in loblolly pine.

Keywords Expressed sequence tag (EST)Denaturing gradient gel electrophoresis (DGGE)Genetic markerGenetic linkage mapLoblolly pine (Pinus taeda L.)

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • B. Temesgen
    • 1
  • G. R. Brown
    • 1
  • D. E. Harry
    • 1
  • C. S. Kinlaw
    • 1
  • M. M. Sewell
    • 1
  • D. B. Neale
    • 1
  1. 1.Institute of Forest Genetics, Pacific Southwest Research Station, USDA Forest Service, c/o Department of Environmental Horticulture, University of California, Davis, CA 95616, USA e-mail: dneale@dendrome.ucdavis.edu Tel.: +1-530-754-8431, Fax: +1-530-754-9366, Present address: B. Temesgen, California Department of Justice, DNA Laboratory, 626 Bancroft Way, Berkeley, CA 94710, USA, Present address: D.E. Harry, Nicholas Turkey Breeding Farms, 19449 Riverside Drive, P.O. Box Y, Sonoma, CA 95476, USA, Present address: M.M. Sewell, Oak Ridge National Laboratory, Environmental Sciences Division, P.O. Box 2088, Oak Ridge, TN37831-2008, USAUS