Original Article

Theoretical and Applied Genetics

, Volume 101, Issue 8, pp 1299-1306

AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.)

  • E. Flores BerriosAffiliated withDepartment of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81
  • , L. GentzbittelAffiliated withDepartment of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81
  • , H. KayyalAffiliated withDepartment d’Amélioration des Plantes, Faculté d’Agriculture, Université de Damas, Syrie
  • , G. AlibertAffiliated withDepartment of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81
  • , A. SarrafiAffiliated withDepartment of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81

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Abstract 

Genetic control for two in vitro organogenesis traits, the number of shoots per explant plated (S/E) and the number of shoots per regenerating explant (S/RE), was investigated in 75 recombinant inbred lines (RILs) of sunflower and their two parents (PAC-2 and RHA-266). Genetic variability was observed among the 75 RILs for the organogenesis traits studied. Some RILs presented significant differences when compared with the best parental line (RHA-266). Genetic gain, in terms of the percentage of the best parent, for 32% of the selected RILs was significant. A set of 99RILs from the same cross including the 75 mentioned above was screened with 333 AFLP markers and a linkage map was constructed based on 264 linked loci. Six putative QTLs for the S/RE (tentatively named osr) and seven QTLs for the S/E (ose) trait were detected using composite interval mapping. For each trait, the QTLs explained 52% (ose) and 67% (osr) of the total phenotypic variance. These results suggested that additive gene effects predominate in explaining a large proportion of the observed genetic variation associated with regeneration ability. The coincidental location of QTLs for S/E and S/RE is discussed.

Keywords Sunflower Helianthus annuus L. AFLP Genetic mapping in vitro regeneration Recombinant inbred lines