Theoretical and Applied Genetics

, Volume 101, Issue 5, pp 984–989

Development of simple sequence repeats (SSRs) in olive tree (Olea europaea L.)

  • P. Rallo
  • G. Dorado
  • A. Martín

DOI: 10.1007/s001220051571

Cite this article as:
Rallo, P., Dorado, G. & Martín, A. Theor Appl Genet (2000) 101: 984. doi:10.1007/s001220051571

Abstract 

We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied in a complex population from a cross between the commercial cultivars ’Leccino’ and ’Dolce Agogia’.

Key words Microsatellites Variable number of tandem repeats (VNTRs) Enriched library Hexamer repeat Null allele Marker-assisted selection (MAS) 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • P. Rallo
    • 1
  • G. Dorado
    • 2
  • A. Martín
    • 1
  1. 1.Instituto de Agricultura Sostenible-CSIC, Alameda del Obispo s/n., P.O. box 4084. 14080-Córdoba, Spain e-mail: ge2ramop@uco.es Fax: + 34-957 499252ES
  2. 2.Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Córdoba, SpainES