Theoretical and Applied Genetics

, Volume 101, Issue 4, pp 580–589

Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

  • T. Toojinda
  • L. H. Broers
  • X. M. Chen
  • P. M. Hayes
  • A. Kleinhofs
  • J. Korte
  • D. Kudrna
  • H. Leung
  • R. F. Line
  • W. Powell
  • L. Ramsay
  • H. Vivar
  • R. Waugh
Original paper

DOI: 10.1007/s001220051519

Cite this article as:
Toojinda, T., Broers, L., Chen, X. et al. Theor Appl Genet (2000) 101: 580. doi:10.1007/s001220051519

Abstract 

Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two- locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

Key words BarleyGenome mappingStripe rustLeaf rustBYDVResistance Gene Analog PolymorphismQTL

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • T. Toojinda
    • 1
  • L. H. Broers
    • 2
  • X. M. Chen
    • 3
  • P. M. Hayes
    • 4
  • A. Kleinhofs
    • 5
  • J. Korte
    • 6
  • D. Kudrna
    • 5
  • H. Leung
    • 7
  • R. F. Line
    • 3
  • W. Powell
    • 8
  • L. Ramsay
    • 8
  • H. Vivar
    • 9
  • R. Waugh
    • 8
  1. 1.DNA Fingerprinting Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kampangsaen Campus, Nakorn Pathom, ThailandTH
  2. 2.Nunhems Zaden BV, P.O. Box 4005, 6080 AA Haelen, the NetherlandsNL
  3. 3.US Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164–6430, USAUS
  4. 4.Department of Crop and Soil Sciences, Oregon State University, Corvallis, OR 97331, USA e-mail: patrick.m.hayes@orst.edu, Fax:+1-541-737-1589US
  5. 5.Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164–6420, USAUS
  6. 6.AgriBioTech, 530 Liberty lane, West Kingston, RI 02892, USAUS
  7. 7.IRRI, P.O. Box933, Manila, 1099, PhilippinesPH
  8. 8.Scottish Crop Research Institute Invergowrie, Dundee DD2 5DA, ScotlandGB
  9. 9.Barley program, ICARDA/CIMMYT, Apdo. 370, Mexico 6, D.F. MexicoMX