Original paper

Theoretical and Applied Genetics

, Volume 101, Issue 4, pp 580-589

First online:

Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare)

  • T. ToojindaAffiliated withDNA Fingerprinting Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kampangsaen Campus, Nakorn Pathom, Thailand
  • , L. H. BroersAffiliated withNunhems Zaden BV, P.O. Box 4005, 6080 AA Haelen, the Netherlands
  • , X. M. ChenAffiliated withUS Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164–6430, USA
  • , P. M. HayesAffiliated withDepartment of Crop and Soil Sciences, Oregon State University, Corvallis, OR 97331, USA e-mail: patrick.m.hayes@orst.edu, Fax:+1-541-737-1589
  • , A. KleinhofsAffiliated withDepartment of Crop and Soil Sciences, Washington State University, Pullman, WA 99164–6420, USA
  • , J. KorteAffiliated withAgriBioTech, 530 Liberty lane, West Kingston, RI 02892, USA
  • , D. KudrnaAffiliated withDepartment of Crop and Soil Sciences, Washington State University, Pullman, WA 99164–6420, USA
  • , H. LeungAffiliated withIRRI, P.O. Box933, Manila, 1099, Philippines
  • , R. F. LineAffiliated withUS Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164–6430, USA
    • , W. PowellAffiliated withScottish Crop Research Institute Invergowrie, Dundee DD2 5DA, Scotland
    • , L. RamsayAffiliated withScottish Crop Research Institute Invergowrie, Dundee DD2 5DA, Scotland
    • , H. VivarAffiliated withBarley program, ICARDA/CIMMYT, Apdo. 370, Mexico 6, D.F. Mexico
    • , R. WaughAffiliated withScottish Crop Research Institute Invergowrie, Dundee DD2 5DA, Scotland

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Abstract 

Stripe rust, leaf rust, and Barley Yellow Dwarf Virus (BYDV) are important diseases of barley (Hordeum vulgare L). Using 94 doubled-haploid lines (DH) from the cross of Shyri x Galena, multiple disease phenotype datasets, and a 99-marker linkage map, we determined the number, genome location, and effects of genes conferring resistance to these diseases. We also mapped Resistance Gene Analog Polymorphism (RGAP) loci, based on degenerate motifs of cloned disease resistance genes, in the same population. Leaf rust resistance was determined by a single gene on chromosome 1 (7H). QTLs on chromosomes 2 (2H), 3 (3H), 5 (1H), and 6 (6H) were the principal determinants of resistance to stripe rust. Two- locus QTL interactions were significant determinants of resistance to this disease. Resistance to the MAV and PAV serotypes of BYDV was determined by coincident QTLs on chromosomes 1 (7H), 4 (4H), and 5 (1H). QTL interactions were not significant for BYDV resistance. The associations of molecular markers with qualitative and quantitative disease resistance loci will be a useful information for marker-assisted selection.

Key words Barley Genome mapping Stripe rust Leaf rust BYDV Resistance Gene Analog Polymorphism QTL