Theoretical and Applied Genetics

, Volume 100, Issue 8, pp 1176–1182

Mapping quantitative trait loci (QTLs) for resistance to Cercospora leaf spot disease (Cercospora beticola Sacc.) in sugar beet (Beta vulgaris L.)

  • A. Setiawan
  • G. Koch
  • S. R. Barnes
  • C. Jung

DOI: 10.1007/s001220051421

Cite this article as:
Setiawan, A., Koch, G., Barnes, S. et al. Theor Appl Genet (2000) 100: 1176. doi:10.1007/s001220051421

Abstract 

The breeding of sugar beet varieties that combine resistance to Cercospora and high yield under non-diseased conditions is a major challenge to the breeder. The understanding of the quantitative trait loci (QTLs) contributing to Cercospora resistance offers one route to solving this problem. A QTL analysis of Cercospora resistance in sugar beet was carried out using a linkage map based on AFLP and RFLP markers. Two different screening methods for Cercospora resistance (a field test at Copparo, Italy, under natural infection, and a newly-developed leaf disc test) were used to estimate the level of Cercospora resistance; the correlation between scores from the field (at 162 days after sowing) and the leaf disc test was significant. QTL analysis was based on F2 and F3 (half-sib family) generations derived from crosses between diploid single plants of 93164P (resistant to Cercospora leaf spot disease) and 95098P (susceptible). Four QTLs associated with Cercospora resistance (based on Lsmean data of the leaf disc test) on chromosomes III, IV, VII and IX were revealed using Composite interval mapping. To produce populations segregating for leaf spot resistance as a single Mendelian factor, we selected for plants heterozygous for only one of the QTLs (on chromosome IV or IX) but homozygous for the others.

Key words Beta vulgarisCercospora beticolaAFLPQTL

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • A. Setiawan
    • 1
  • G. Koch
    • 1
  • S. R. Barnes
    • 2
  • C. Jung
    • 1
  1. 1.Institute of Crop Science and Plant Breeding, Christian-Albrechts-University Kiel, Olshausenstr. 40, D-24118 Kiel, Germany e-mail: cjung@plantbreeding.uni-kiel.deDE
  2. 2.SES Europe, Industriepark 15, 3300 Tienen, BelgiumBE
  3. 3.A. Dieckmann-Heimberg, Saatsucht Sülbeck, Postfach 1165, D-31684 Nienstadt, GermanyDE