Theoretical and Applied Genetics

, Volume 100, Issue 7, pp 1121–1128

Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice

Authors

  • S. Hittalmani
    • International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in
  • A. Parco
    • International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in
  • T. V. Mew
    • International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in
  • R. S. Zeigler
    • International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in
  • N. Huang
    • International Rice Research Institute, P.O. Box 933, Manila, Philippines e-mail: maslab@satyam.net.in
Original paper

DOI: 10.1007/s001220051395

Cite this article as:
Hittalmani, S., Parco, A., Mew, T. et al. Theor Appl Genet (2000) 100: 1121. doi:10.1007/s001220051395

Abstract 

Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf blast in the Philippines and India indicated that combinations including Piz-5 have enhanced resistance than when it is present alone. The genes from the pyramided lines are at present being deployed into agronomically superior ricevarieties by marker-aided selection (MAS).

Key words RFLP markersFine-mappingBlast resistancePyramidingMarker-assisted selection
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Copyright information

© Springer-Verlag Berlin Heidelberg 2000