Original article

Theoretical and Applied Genetics

, Volume 99, Issue 3, pp 656-662

First online:

Comparative mapping in loblolly and radiata pine using RFLP and microsatellite markers

  • M. E. DeveyAffiliated withCSIRO Forestry and Forest Products
  • , M. M. SewellAffiliated withUSDA Forest Service, Institute of Forest Genetics, Department of Environmental Horticulture, University of California
  • , T. L. UrenAffiliated withCSIRO Forestry and Forest Products
  • , D. B. NealeAffiliated withUSDA Forest Service, Institute of Forest Genetics, Department of Environmental Horticulture, University of California

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Abstract 

Genetic linkage maps were constructed for loblolly pine (Pinus taeda L.) and radiata pine (P. radiata D. Don) using a common set of RFLP and microsatellite markers. The map for loblolly pine combined data from two full-sib families and consisted of 20 linkage groups covering 1281 cM. The map for radiata pine had 14 linkage groups and covered 1223 cM. All of the RFLP probes readily hybridise between loblolly and radiata pine often producing similar hybridisation patterns. There were in total 60 homologous RFLP loci mapped in both species which could be used for comparative purposes. A set of 20 microsatellite markers derived from radiata pine were also assayed; however, only 9 amplified and revealed polymorphic loci in both species. Single-locus RFLP and microsatellite markers were used to match up linkage groups and compare order between species. Twelve syntenic groups were obtained each consisting of from 3 to 9 homologous loci. The order of homologous loci was colinear in most cases, suggesting no major chromosomal rearrangements in the evolution of these species. Comparative mapping between loblolly and radiata pine should facilitate genetic research in both species and provide a framework for mapping in other pine species.

Key words Comparative mapping RFLP Microsatellite markers Loblolly pine Radiata pine