Theoretical and Applied Genetics

, Volume 99, Issue 3, pp 383–390

Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase

Authors

  • Y. Nishizawa
    • National Institute of Agrobiological Resources, 2-1-2,Kannondai
  • Z. Nishio
    • National Institute of Agrobiological Resources, 2-1-2,Kannondai
  • K. Nakazono
  • M. Soma
    • Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi
  • E. Nakajima
    • National Institute of Agrobiological Resources, 2-1-2,Kannondai
  • M. Ugaki
    • National Institute of Agrobiological Resources, 2-1-2,Kannondai
  • T. Hibi
    • Department of Agricultural and Environmental Biology, The University of Tokyo, 1-1-1, Yayoi
Article

DOI: 10.1007/s001220051248

Cite this article as:
Nishizawa, Y., Nishio, Z., Nakazono, K. et al. Theor Appl Genet (1999) 99: 383. doi:10.1007/s001220051248

Abstract 

Rice blast is the most devastating plant disease in Japan. Our goal is to create new rice varieties which show enhanced resistance against blast, regardless of the race of blast. By an Agrobacterium-mediated transformation method, we reintroduced a rice class-I chitinase gene, Cht-2 or Cht-3, under the control of the enhanced CaMV 35S promoter and a hygromycin phosphotransferase gene, as a selection marker into the Japonica rice varieties Nipponbare and Koshihikari, which have retained the best popularity over a long period in Japan. In regenerated plants (R0), the Cht-2 product was found to accumulate intracellularly whereas the Cht-3 product was found to be targeted extracellularly. The transgenic rice plants which constitutively expressed either chitinase gene showed significantly higher resistance against the rice blast pathogen Magnaporthe grisea races 007.0 and 333. Both high-level expression of the chitinase and blast-resistance were stably inherited by the next generation in several lines.

Key words Blast disease-resistanceChitinaseNon-race-specific resistanceOverexpressionTransgenic rice

Copyright information

© Springer-Verlag Berlin Heidelberg 1999