Theoretical and Applied Genetics

, Volume 98, Issue 6, pp 1163–1170

Genetic linkage map of a wheat×spelt cross

  • M. M. Messmer
  • M. Keller
  • S. Zanetti
  • B. Keller

DOI: 10.1007/s001220051181

Cite this article as:
Messmer, M., Keller, M., Zanetti, S. et al. Theor Appl Genet (1999) 98: 1163. doi:10.1007/s001220051181

Abstract

 We constructed a genetic map of a cross between the Swiss winter wheat (Triticum aestivum L.) variety Forno and the Swiss winter spelt (Triticum spelta L.) variety Oberkulmer. For the linkage analysis,176 polymorphic RFLP probes and nine microsatellites were tested on 204 F5 recombinant inbred lines (RILs) of Forno×Oberkulmer revealing 242 segregating marker loci. Thirty five percent of these loci showed significant (P>0.05) deviation from a 1 : 1 segregation, and the percentage of Forno alleles ranged from 21% to 83% for individual marker loci. Linkage analysis was performed with the program MAPMAKER using the Haldane mapping function. Using a LOD threshold of 10, we obtained 37 linkage groups. After finding the best order of marker loci within linkage groups by multi-point analysis we assembled the linkage groups into 23 larger units by lowering the LOD threshold. All except one of the 23 new linkage groups could be assigned to physical chromosomes or chromosome arms according to hybridisation patterns of nulli-tetrasomic lines of Chinese Spring and published wheat maps. This resulted in a genetic map comprising 230 marker loci and spanning 2469 cM. Since the analysed population is segregating for a wide range of agronomically important traits, this genetic map is an ideal basis for the identification of quantitative trait loci (QTLs) for these traits.

Key wordsTriticum aestivumTriticum speltaRFLPGenetic mapQTL

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. M. Messmer
    • 1
  • M. Keller
    • 1
  • S. Zanetti
    • 1
  • B. Keller
    • 3
  1. 1.Swiss Federal Research Station for Agroecology and Agriculture (FAL) Zürich-Reckenholz, Reckenholzstrasse 191, CH-8046 Zürich, Switzerland Tel.: +41-1-3777 445 Fax: +41-1-3777 201 E-mail: monika.messmer@fal.admin.chCH
  2. 2.Institute of Plant Science, Swiss Federal Institute of Technology (ETH), Universitätsstrasse 2, CH-8092 Zürich, SwitzerlandCH
  3. 3.Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, SwitzerlandCH