Theoretical and Applied Genetics

, Volume 97, Issue 5, pp 905-915

First online:

Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum

  • V. LaucouAffiliated withStation de Génétique et d’Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France Fax :+3-1-30 83 33 19 E-mail: rameau@versailles.inra.fr
  • , K. HaurognéAffiliated withStation de Génétique et d’Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France Fax :+3-1-30 83 33 19 E-mail: rameau@versailles.inra.fr
  • , N. EllisAffiliated withDepartment of Applied Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
  • , C. RameauAffiliated withStation de Génétique et d’Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France Fax :+3-1-30 83 33 19 E-mail: rameau@versailles.inra.fr

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Abstract

 A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed. For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.

Key words Linkage mapping Pisum sativum Random amplified polymorphic DNA Recombinant inbred lines