Theoretical and Applied Genetics

, Volume 97, Issue 1, pp 83–89

A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines

  • I. Eujayl
  • M. Baum
  • W. Powell
  • W. Erskine
  • E. Pehu

DOI: 10.1007/s001220050869

Cite this article as:
Eujayl, I., Baum, M., Powell, W. et al. Theor Appl Genet (1998) 97: 83. doi:10.1007/s001220050869

Abstract

 A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of 6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion. More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification of markers linked to quantitative trait loci in this population.

Key words Lens culinaris subsp. orientalisRecombinant inbred linesAFLPRAPDGenetic map

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • I. Eujayl
    • 1
  • M. Baum
    • 1
  • W. Powell
    • 2
  • W. Erskine
    • 1
  • E. Pehu
    • 3
  1. 1.Biotechnology, Germplasm Program, ICARDA, P.O. Box 5466, Aleppo, Syria e-mail: m.baum@cgnet.comXX
  2. 2.Cellular and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UKGB
  3. 3.Department of Plant Production, PL 27 (Viikki), 00014 University of Helsinki, FinlandFI