Theoretical and Applied Genetics

, Volume 96, Issue 5, pp 602–611

AFLP markers for the study of rice biodiversity

  • J. Zhu
  • M. D. Gale
  • S. Quarrie
  • M. T. Jackson
  • G. J. Bryan

DOI: 10.1007/s001220050778

Cite this article as:
Zhu, J., Gale, M., Quarrie, S. et al. Theor Appl Genet (1998) 96: 602. doi:10.1007/s001220050778

Abstract

 AFLP was used as a DNA fingerprinting technique in rice (Oryza sativa L.) germplasm analysis. The high efficiency and random coverage of AFLP markers were established. With only five combinations of primers and RFLP anchors, a framework linkage map was constructed. This map demonstrated that the AFLP markers from a limited number of primers were not confined to any particular regions or chromosomes in the rice genome. To analyse the biodiversity of 57 rice germplasm accessions, we examined 179 polymorphic AFLP markers generated from four primer combinations. Both principal component analysis and cluster analysis were used, and three groups were clearly identified which corresponded to genotypes of Isozyme Groups I, II and VI. The number of markers needed for robust classification of rice germplasm and the diversity between/within the groups was established.

Key words AFLP Rice Biodiversity Germplasm Mapping 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. Zhu
    • 1
  • M. D. Gale
    • 1
  • S. Quarrie
    • 1
  • M. T. Jackson
    • 2
  • G. J. Bryan
    • 1
  1. 1.John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK Fax: +44-01603 502241 E-mail: zhu@bbsrc.ac.ukGB
  2. 2.International Rice Research Institute, PO Box 933, 1099 Manila, PhilippinesXX

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