Theoretical and Applied Genetics

, Volume 96, Issue 1, pp 95–100

Potential of doubled-haploid lines and localization of quantitative trait loci (QTL) for partial resistance to bacterial leaf streak (Xanthomonas campestris pv. hordei) in barley

  • H. El Attari
  • P. M. Hayes
  • A. Rebai
  • G. Barrault
  • G. Dechamp-Guillaume
  • A. Sarrafi

DOI: 10.1007/s001220050714

Cite this article as:
Attari, H., Hayes, P., Rebai, A. et al. Theor Appl Genet (1998) 96: 95. doi:10.1007/s001220050714

Abstract

 Genetic variability for partial resistance to bacterial leaf streak in barley, caused by Xanthomonas campestris pv. hordei, was investigated in 119 doubled-haploid lines (DH) developed by the Hordeum bulbosum method from the F1 progeny of the cross between two cultivars, ‘Morex’ (resistant) and ‘Steptoe’ (susceptible). Two experiments were undertaken in a randomized complete block design with three replicates, in a controlled growth chamber. Twenty seeds per replicate were planted in plastic containers (60×40×8 cm) containing moistened vermiculite. At the two-leaf stage seedlings were inoculated with an Iranian strain of the pathogen. Genetic variability was observed among the 119 DH lines for partial resistance to the disease. Some DH lines were significantly more resistant than ‘Morex’ (resistant parent) to bacterial leaf streak. Genetic gain in percentage of resistant parent for 5% of the selected DH lines was significant (47.70% and 33.72% in the first and the second experiment, respectively). A QTL analysis of bacterial leaf streak resistance showed that three QTLs were detected on chromosomes 3 and 7. Multilocus allelic effects of the three QTLs account for almost 54% of the mean difference between the parents and nearly 30% of the phenotypic variation of the trait in the mean experiment. The resistance locus on chromosome 3, near ABG377, apprears to be a major gene.

Key words Quantitative trait lociBarleyDoubled haploidPartial resistanceXanthomonas campestris pv. hordei

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. El Attari
    • 1
  • P. M. Hayes
    • 2
  • A. Rebai
    • 1
  • G. Barrault
    • 1
  • G. Dechamp-Guillaume
    • 1
  • A. Sarrafi
    • 1
  1. 1.Ecole Nationale Supérieure Agronomique de Toulouse, Institut National Polytechnique (ENSAT-INP), 145, Avenue de Muret, F-31076 Toulouse-Cédex, France Fax: +33(0)562136564 E-Mail: sarrafi@ensat.fr.FR
  2. 2.Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USAUS