Theoretical and Applied Genetics

, Volume 95, Issue 8, pp 1289–1299

A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs)

  • B. J. Gilpin
  • J. A. McCallum
  • T. J. Frew
  • G. M. Timmerman-Vaughan

DOI: 10.1007/s001220050695

Cite this article as:
Gilpin, B., McCallum, J., Frew, T. et al. Theor Appl Genet (1997) 95: 1289. doi:10.1007/s001220050695

Abstract

 A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of 29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed for single-copy sequences. Eleven polymorphic STSs were developed.

Key words Pisum sativumLinkage mappingDNA markersExpressed sequence tagsSequence-tagged sites

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • B. J. Gilpin
    • 1
  • J. A. McCallum
    • 1
  • T. J. Frew
    • 1
  • G. M. Timmerman-Vaughan
    • 1
  1. 1.New Zealand Institute for Crop and Food Research Ltd., Private Bag 4704, Christchurch, New ZealandNZ