Theoretical and Applied Genetics

, Volume 94, Issue 1, pp 39–45

Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population

  • M. Maheswaran
  • P. K. Subudhi
  • S. Nandi
  • J. C. Xu
  • A. Parco
  • D. C. Yang
  • N. Huang
Article

DOI: 10.1007/s001220050379

Cite this article as:
Maheswaran, M., Subudhi, P.K., Nandi, S. et al. Theoret. Appl. Genetics (1997) 94: 39. doi:10.1007/s001220050379

Abstract

We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with AFLP markers they will be very useful for marker-assisted backcrossing.

Key words

Genetic mapMarker-aided selectionOryza sativaRFLP markers

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • M. Maheswaran
    • 1
  • P. K. Subudhi
    • 1
  • S. Nandi
    • 1
  • J. C. Xu
    • 1
  • A. Parco
    • 1
  • D. C. Yang
    • 1
  • N. Huang
    • 1
  1. 1.International Rice Research InstituteManilaPhilippines