Theoretical and Applied Genetics

, Volume 100, Issue 1, pp 75–81

An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations

  • R. L. Sebastian
  • E. C. Howell
  • G. J. King
  • D. F. Marshall
  • M. J. Kearsey
Original Article

DOI: 10.1007/s001220050011

Cite this article as:
Sebastian, R., Howell, E., King, G. et al. Theor Appl Genet (2000) 100: 75. doi:10.1007/s001220050011

Abstract 

Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.

Key words Brassica oleraceaIntegrated mapMolecular markersDoubled-haploidComparative mapping

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • R. L. Sebastian
    • 1
  • E. C. Howell
    • 1
  • G. J. King
    • 2
  • D. F. Marshall
    • 1
  • M. J. Kearsey
    • 1
  1. 1.Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UKGB
  2. 2.Horticulture Research International, Wellesbourne, Warwickshire, CV35 9EF, UKGB