Theoretical and Applied Genetics

, Volume 102, Issue 6, pp 847–852

Molecular cytogenetic evidence for a high level of chromosome pairing among different genomes in Triticum aestivum–Thinopyrum intermedium hybrids

  • Q. Chen
  • R. L. Conner
  • A. Laroche
  • F. Ahmad
Original Paper

DOI: 10.1007/s001220000496

Cite this article as:
Chen, Q., Conner, R., Laroche, A. et al. Theor Appl Genet (2001) 102: 847. doi:10.1007/s001220000496

Abstract 

Intergeneric hybrids (ABDJJsS genomes) were made between Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium. Genomic in situ hybridization (GISH) using genomic DNA probes from Pseudoroegneria libanotica (Hackel) D.R. Dewey (genome S, 2n = 14) was used to study chromosome pairing among J, Js, S and wheat ABD genomes in the hybrids. It was shown that in the hexaploid (ABDJJsS) hybrids, high pairing occurred among wheat chromosomes and among Thinopyrum chromosomes. A closer relationship was observed among the three genomes of Th. intermedium than among the three genomes of T. aestivum. It was further discerned that S genome chromosomes paired with J- and Js-genome chromosomes at a high frequency. The frequency of heterologous pairing between S and J or S and Js chromosomes was higher than those between J and Js chromosomes, indicating that the S-genome was more closely related with these two genomes. Our results provided direct molecular cytogenetic evidence for the hypothesis that S-genome chromosomes are genetically similar to the J-genome chromosomes and, therefore, genetic exchange between these genomes is possible. The discovery of a close relationship among S, J and Js genomes provides valuable markers for molecular cytogenetic analyses using S-genomic DNA probes in monitoring the transfer of useful traits from Thinopyrum species into wheat.

Keywords Genomic in situ hybridization Thinopyrum intermedium Triticum aestivum Js and S genomes Chromosome pairing 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Q. Chen
    • 1
  • R. L. Conner
    • 1
  • A. Laroche
    • 1
  • F. Ahmad
    • 2
  1. 1.Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge AB T1J 4B1, Canada e-mail: Chenqi@em.agr.ca Fax: +1(403) 382-3156CA
  2. 2.Botany Department, Brandon University, Brandon MB R7A 6A9, Canada LRC Contribution No. 3870051CA

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