Theoretical and Applied Genetics

, Volume 127, Issue 6, pp 1449–1459

Mapping of Yr62 and a small-effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252

  • Yan Lu
  • Meinan Wang
  • Xianming Chen
  • Deven See
  • Shiaoman Chao
  • Jinxue Jing
Original Paper

DOI: 10.1007/s00122-014-2312-0

Cite this article as:
Lu, Y., Wang, M., Chen, X. et al. Theor Appl Genet (2014) 127: 1449. doi:10.1007/s00122-014-2312-0

Abstract

Key message

This manuscript reports a new gene (Yr62) and a small-effect QTL for potentially durable resistance to stripe rust and usefulness ofYr62markers for marker-assisted selection.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease of wheat worldwide. Spring wheat germplasm PI 192252 showed a high level of high-temperature adult-plant (HTAP) resistance to stripe rust in germplasm evaluation over 8 years in the State of Washington. To elucidate the genetic basis of resistance, PI 192252 was crossed with ‘Avocet susceptible’. A mapping population of 150 F5 recombinant inbred lines was developed using single-seed descent. Stripe rust tests were conducted with selected Pst races in a greenhouse and in field conditions under natural infections. The relative area under the disease progress curve (rAUDPC) data showed continuous distributions, indicating that HTAP resistance of PI 192252 was controlled by quantitative trait loci (QTL). Two QTL were identified in PI 192252, explaining 74.2 % of the total phenotypic variation for rAUDPC. These two QTL were mapped to chromosomes 4BL (QYrPI192252.wgp-4BL) and 5BS (QYrPI192252.wgp-5BS) with SSR and SNP markers and explained 40–60 and 22–27 %, respectively, of the phenotypic variation across the four environments. Because the major-effect QTL on 4BL is different from previously named Yr genes and inherited as a single gene, it is named Yr62. The SSR marker alleles Xgwm192222 and Xgwm251133 flanking Yr62 were different from the alleles in various wheat varieties, suggesting that these markers could be useful in marker-assisted selection for incorporating Yr62 into commercial cultivars.

Supplementary material

122_2014_2312_MOESM1_ESM.docx (24 kb)
Supplementary Table 1 (DOCX 24 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2014

Authors and Affiliations

  • Yan Lu
    • 1
    • 2
  • Meinan Wang
    • 2
  • Xianming Chen
    • 2
    • 3
  • Deven See
    • 2
    • 3
  • Shiaoman Chao
    • 4
  • Jinxue Jing
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant ProtectionNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Department of Plant PathologyWashington State UniversityPullmanUSA
  3. 3.US Department of AgricultureAgricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research UnitPullmanUSA
  4. 4.US Department of AgricultureAgricultural Research Service, Cereal Crops ResearchFargoUSA