Theoretical and Applied Genetics

, Volume 127, Issue 2, pp 481–488

Mapping and identification of a Cicer arietinumNSP2 gene involved in nodulation pathway

  • L. Ali
  • E. Madrid
  • R. K. Varshney
  • S. Azam
  • T. Millan
  • J. Rubio
  • J. Gil
Original Paper

DOI: 10.1007/s00122-013-2233-3

Cite this article as:
Ali, L., Madrid, E., Varshney, R.K. et al. Theor Appl Genet (2014) 127: 481. doi:10.1007/s00122-013-2233-3

Abstract

Key message

For the first time the putativeNSP2gene in chickpea has been identified using pairs of NILs differing for theRn1/rn1nodulation gene that was located in LG5 of chickpea genetic map.

Abstract

An intraspecific cross between the mutant non-nodulating genotype PM233, carrying the recessive gene rn1, and the wild-type CA2139 was used to develop two pairs of near-isogenic lines (NILs) for nodulation in chickpea. These pairs of NILs were characterized using sequence tagged microsatellite site (STMS) markers distributed across different linkage groups (LGs) of the chickpea genetic map leading to the detection of polymorphic markers located in LG5. Using this information, together with the genome annotation in Medicago truncatula, a candidate gene (NSP2) known to be involved in nodulation pathway was selected for mapping in chickpea. The full length sequence obtained in chickpea wild-type (CaNSP2) was 1,503 bp. Linkage analysis in an F3 population of 118 plants derived from the cross between the pair of NILS NIL7-2A (nod) × NIL7-2B (non-nod) revealed a co-localization between CaNSP2 and Rn1 gene. These data implicate the CaNSP2 gene as a candidate for identity to Rn1, and suggest that it could act in the nodulation signaling transduction pathway similarly to that in other legumes species.

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • L. Ali
    • 1
  • E. Madrid
    • 2
  • R. K. Varshney
    • 3
  • S. Azam
    • 3
  • T. Millan
    • 1
  • J. Rubio
    • 4
  • J. Gil
    • 1
  1. 1.Dpto GenéticaUniv Córdoba, Campus de excelencia internacional CeiA3, Campus de RabanalesCórdobaSpain
  2. 2.Institute for Sustainable Agriculture, CSICCórdobaSpain
  3. 3.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)HyderabadIndia
  4. 4.Área de Mejora y BiotecnologíaIFAPA Centro “Alameda del Obispo”CórdobaSpain

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