Theoretical and Applied Genetics

, Volume 124, Issue 5, pp 893–902

Mapping and characterization of seed dormancy QTLs using chromosome segment substitution lines in rice

Authors

  • Salem Marzougui
    • University of Tsukuba
    • National Institute of Agrobiological Sciences
    • National Institute of Agrobiological Sciences
  • Utako Yamanouchi
    • National Institute of Agrobiological Sciences
  • Masaki Shimono
    • National Institute of Agrobiological Sciences
  • Tomoki Hoshino
    • National Institute of Agrobiological Sciences
  • Kiyosumi Hori
    • National Institute of Agrobiological Sciences
  • Masatomo Kobayashi
    • BioResources Center, RIKEN
  • Kanako Ishiyama
    • BioResources Center, RIKEN
  • Masahiro Yano
    • University of Tsukuba
    • National Institute of Agrobiological Sciences
Original Paper

DOI: 10.1007/s00122-011-1753-y

Cite this article as:
Marzougui, S., Sugimoto, K., Yamanouchi, U. et al. Theor Appl Genet (2012) 124: 893. doi:10.1007/s00122-011-1753-y

Abstract

Seed dormancy—the temporary failure of a viable seed to germinate under favorable conditions—is a complex characteristic influenced by many genes and environmental factors. To detect the genetic factors associated with seed dormancy in rice, we conducted a QTL analysis using chromosome segment substitution lines (CSSLs) derived from a cross between Nona Bokra (strong dormancy) and Koshihikari (weak dormancy). Comparison of the levels of seed dormancy of the CSSLs and their recurrent parent Koshihikari revealed that two chromosomal regions—on the short arms of chromosomes 1 and 6—were involved in the variation in seed dormancy. Further genetic analyses using an F2 population derived from crosses between the CSSLs and Koshihikari confirmed the allelic differences and the chromosomal locations of three putative QTLs: Sdr6 on chromosome 1 and Sdr9 and Sdr10 on chromosome 6. The Nona Bokra alleles of the three QTLs were associated with decreased germination rate. We discuss the physiological features of the CSSLs and speculate on the possible mechanisms of dormancy in light of the newly detected QTLs.

Copyright information

© Springer-Verlag 2011