Theoretical and Applied Genetics

, Volume 124, Issue 1, pp 1–11

Genetic analysis of adult plant, quantitative resistance to stripe rust in wheat cultivar ‘Stephens’ in multi-environment trials

  • M. Dolores Vazquez
  • C. James Peterson
  • Oscar Riera-Lizarazu
  • Xianming Chen
  • Adam Heesacker
  • Karim Ammar
  • Jose Crossa
  • Christopher C. Mundt
Original Paper

DOI: 10.1007/s00122-011-1681-x

Cite this article as:
Dolores Vazquez, M., James Peterson, C., Riera-Lizarazu, O. et al. Theor Appl Genet (2012) 124: 1. doi:10.1007/s00122-011-1681-x

Abstract

The wheat (Triticum aestivum L.) cultivar ‘Stephens’ has been grown commercially in the USA Pacific Northwest for 30 years. The durable resistance of ‘Stephens’ to stripe rust (Puccinia striiformis f. sp. tritici) was believed to be due to a combination of seedling and adult plant resistance genes. Multilocation field trials, diversity array technology (DArT), and simple sequence repeat (SSR) markers were used to identify quantitative trait loci (QTL) for resistance. Recombinant inbred lines were assessed for stripe rust response in eight locations/years, five in 2008 and three in 2009. The data from Mt. Vernon, WA, differed from all other environments, and composite interval mapping (CIM) identified three QTL, QYrst.orr-1AL, QYrst.orr-4BS, and QYrpl.orr-6AL, which accounted for 12, 11, and 6% of the phenotypic variance, respectively. CIM across the remaining six environments identified four main QTL. Two QTL, QYrst.orr-2BS.2 and QYrst.orr-7AS, were detected in five of six environments and explained 11 and 15% of the phenotypic variance, respectively. Two other QTL, QYrst.orr-2AS and QYrpl.orr-4BL, were detected across four and three of six environments, and explained 19 and 9% of the phenotypic variance, respectively. The susceptible parent ‘Platte’ contributed QYrpl.orr-4BL and QYrpl.orr-6AL, with the remaining QTL originating from ‘Stephens’. For each environment, additional minor QTL were detected, each accounting for 6–10% of the phenotypic variance. Different QTL with moderate effects were identified in both ‘Stephens’ and ‘Platte’. Significant QTL × environment interactions were evident, suggesting that specificity to plant stage, pathogen genotype, and/or temperature was important.

Supplementary material

122_2011_1681_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 44 kb)
122_2011_1681_MOESM2_ESM.pdf (180 kb)
Frequency distributions of stripe rust ratings for 156 RILs derived from ‘Stephens’ x ‘Platte’ in eight environments. The response of the susceptible and resistant parents are shown on each frequency distribution Supplementary material 2 (PDF 180 kb)
122_2011_1681_MOESM3_ESM.pdf (64 kb)
Linkage map of wheat based on the mapping population from a cross between ‘Stephens’ x ‘Platte’ Supplementary material 3 (PDF 63 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • M. Dolores Vazquez
    • 1
  • C. James Peterson
    • 1
    • 6
  • Oscar Riera-Lizarazu
    • 1
    • 7
  • Xianming Chen
    • 2
    • 3
  • Adam Heesacker
    • 1
  • Karim Ammar
    • 4
  • Jose Crossa
    • 4
  • Christopher C. Mundt
    • 5
  1. 1.Department of Crop and Soil ScienceOregon State UniversityCorvallisUSA
  2. 2.US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology, and Disease Research UnitWashington State UniversityPullmanUSA
  3. 3.Department of Plant PathologyWashington State UniversityPullmanUSA
  4. 4.International Maize and Wheat Improvement Center (CIMMYT)Mexico D.F.Mexico
  5. 5.Department of Botany & Plant PathologyOregon State UniversityCorvallisUSA
  6. 6.Limagrain Cereals SeedsFort CollinsUSA
  7. 7.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)PatancheruIndia