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A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat

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Abstract

Stem rust (Puccinia graminis f. sp. tritici Eriks. & E. Henn.) (the causal agent of wheat stem rust) race Ug99 (also designated TTKSK) and its derivatives have defeated several important stem rust resistance genes widely used in wheat (Triticum aestivum L.) production, rendering much of the worldwide wheat acreage susceptible. In order to identify new resistance sources, a large collection of wheat relatives and genetic stocks maintained at the Wheat Genetic and Genomic Resources Center was screened. The results revealed that most accessions of the diploid relative Dasypyrum villosum (L.) Candargy were highly resistant. The screening of a set of wheat–D. villosum chromosome addition lines revealed that the wheat–D. villosum disomic addition line DA6V#3 was moderately resistant to race Ug99. The objective of the present study was to produce and characterize compensating wheat–D. villosum whole arm Robertsonian translocations (RobTs) involving chromosomes 6D of wheat and 6V#3 of D. villosum through the mechanism of centric breakage-fusion. Seven 6V#3-specific EST–STS markers were developed for screening F2 progeny derived from plants double-monosomic for chromosomes 6D and 6V#3. Surprisingly, although 6D was the target chromosome, all recovered RobTs involved chromosome 6A implying a novel mechanism for the origin of RobTs. Homozygous translocations (T6AS·6V#3L and T6AL·6V#3S) with good plant vigor and full fertility were selected from F3 families. A stem rust resistance gene was mapped to the long arm 6V#3L in T6AS·6V#3L and was designated as Sr52. Sr52 is temperature-sensitive and is most effective at 16°C, partially effective at 24°C, and ineffective at 28°C. The T6AS·6V#3L stock is a new source of resistance to Ug99, is cytogenetically stable, and may be useful in wheat improvement.

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Acknowledgments

We thank Drs. Brady Vick and Steven S. Xu for critical review of the manuscript, W. John Raupp for editorial assistance with the manuscript, and Duane L. Wilson for excellent technical help. This research was supported by grants from the Kansas Wheat Commission, the Kansas Crop Improvement Association, a special USDA-CSREES grant to the Wheat Genetic and Genomic Resources Center, and a grant from Cornell University Project “Durable Rust Resistance in Wheat”. This paper is contribution number 11-166-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502.

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Authors and Affiliations

  1. USDA-ARS, Northern Crop Science Lab, Fargo, ND, 58102-2765, USA

    L. L. Qi

  2. Department of Crop and Soil Sciences, Washington State University, 381 Johnson Hall, Pullman, WA, 99164-6420, USA

    M. O. Pumphrey

  3. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    Bernd Friebe, C. Qian & B. S. Gill

  4. Plant Breeding Institute, University of Sydney, 107 Cobbitty Road, Camden, NSW, 2570, Australia

    P. Zhang

  5. USDA-ARS, Hard Winter Wheat Genetics Research Unit, Kansas State University, 4008 Throckmorton Hall, Manhattan, KS, 66506-5502, USA

    R. L. Bowden

  6. USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave, University of Minnesota, St. Paul, MN, 55108, USA

    M. N. Rouse & Y. Jin

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  1. L. L. Qi
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  2. M. O. Pumphrey
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  3. Bernd Friebe
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  5. C. Qian
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  7. M. N. Rouse
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  8. Y. Jin
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  9. B. S. Gill
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Correspondence to Bernd Friebe.

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Communicated by P. Langridge.

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Qi, L.L., Pumphrey, M.O., Friebe, B. et al. A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123, 159–167 (2011). https://doi.org/10.1007/s00122-011-1574-z

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  • DOI: https://doi.org/10.1007/s00122-011-1574-z

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