Theoretical and Applied Genetics

, Volume 123, Issue 1, pp 143–157

Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat

  • Iago Lowe
  • Ljupcho Jankuloski
  • Shiaoman Chao
  • Xianming Chen
  • Deven See
  • Jorge Dubcovsky
Original Paper

DOI: 10.1007/s00122-011-1573-0

Cite this article as:
Lowe, I., Jankuloski, L., Chao, S. et al. Theor Appl Genet (2011) 123: 143. doi:10.1007/s00122-011-1573-0
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Abstract

A mapping population of 186 recombinant inbred lines developed from a cross between UC1110, an adapted California spring wheat, and PI610750, a synthetic derivative from CIMMYT’s Wide Cross Program, was evaluated for its response to current California races of stripe rust (Puccinia striiformis f. sp. tritici) in replicated field trials over four seasons (2007–2010) in the northern Sacramento Valley. A genetic map was constructed consisting of 1,494 polymorphic probes (SSRs, DArTs, and ESTs) mapped to 558 unique loci, and QTL analysis revealed the presence of four stripe rust resistance QTL segregating in this population, two from UC1110 (on chromosomes 3BS and 2BS) and two from PI610750 (5AL and 2AS). The two QTL of largest effects (on 3BS and 5AL) were validated in independent populations and their intervals narrowed to 2.5 and 5.3 cM, respectively. The 3BS QTL was shown, by allelism test and genotype, to carry a gene different from the Yr30/Sr2 complex. Mapped position also suggests that the 3BS QTL is associated with a gene different from either Yrns-B1 or YrRub, two stripe rust resistance genes mapped to this region in other studies. The 5AL QTL carries a previously unreported partial stripe rust resistance gene, designated here as Yr48. This paper discusses the individual contributions to resistance of these four QTL, their epistatic interactions, and their potential in durable resistance breeding strategies based on combinations of partial resistance genes.

Abbreviations

AFLP

Amplified fragment length polymorphism

ANOVA

Analysis of variance

cM

centiMorgan (in this population, Kosambi)

DArT

Diversity array technology

EST

Expressed sequence tag

HTAP

High-temperature adult plant

LOD

Log of the odds

NIL

Near-isogenic line

PST

Puccinia striiformis Westend. f. sp. tritici Eriks

QTL

Quantitative trait locus or quantitative trait loci, depending on context

RIL

Recombinant inbred line

SSR

Simple sequence repeat (microsatellite)

3BS

The short arm of chromosome 3B

5AL

The long arm of chromosome 5A

2BS

The short arm of chromosome 2B

2AS

The short arm of chromosome 2A

Supplementary material

122_2011_1573_MOESM1_ESM.pdf (1.9 mb)
Online Resource 1. Detailed description, with images, of the parental phenotypes and the 11-point reaction type disease evaluation scale used in this study (PDF 1922 kb)
122_2011_1573_MOESM2_ESM.pdf (223 kb)
Online Resource 2. Complete genetic map for the UC1110/PI610750 mapping population, including detailed notes and summary QTL information (PDF 222 kb)
122_2011_1573_MOESM3_ESM.xls (1.5 mb)
Online Resource 3. Searchable spreadsheet of the complete UC1110/PI610750 genetic map, including all genotypic and phenotypic data used in this study; also GSTR assignments of deposited lines (XLS 1.54 mb)
122_2011_1573_MOESM4_ESM.pdf (526 kb)
Online Resource 4. Supplementary figures (S1-S6): Correlation scatterplots among replications within each year, correlation scatterplots between years, LOD plots of the sub-threshold 3D and 7AL QTL regions, QTL interaction plots (11-point reaction type), bar charts of effects of isolated QTL regions (reaction type), and bar chart of effects of 3BS and 5AL QTL regions by year (PDF 526 kb)
122_2011_1573_MOESM5_ESM.pdf (741 kb)
Online Resource 5. Supplementary tables (S1-S3) summarizing validation and dissection data for the 3BS, 5AL, and 2BS QTL regions (PDF 740 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Iago Lowe
    • 1
  • Ljupcho Jankuloski
    • 1
    • 2
  • Shiaoman Chao
    • 3
  • Xianming Chen
    • 4
  • Deven See
    • 4
  • Jorge Dubcovsky
    • 1
  1. 1.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  2. 2.Department of Genetics and Plant BreedingFaculty of Agricultural Sciences and FoodSkopjeRepublic of Macedonia
  3. 3.Cereal Crops Research UnitUSDA-ARSFargoUSA
  4. 4.Wheat Genetics, Quality Physiology, and Disease Research UnitUSDA-ARSPullmanUSA