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Identification of QTL associated with resistance to bacterial spot race T4 in tomato

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Abstract

Bacterial spot of tomato (Solanum lycopersicum L.), caused by several Xanthomonas sp., is a serious but difficult disease to control by chemical means. Development of resistance has been hindered by emergence of races virulent to tomato, by the quantitative inheritance of resistance, and by a low correlation between seedling assays and resistance in the field. Resistance to multiple races, including race T4, has been described in the S. lycopersicum var. cerasiformae accession PI 114490. We used molecular markers to identify associations with quantitative trait loci (QTL) in an elite inbred backcross (IBC) population derived from OH 9242, PI 114490 and Fla. 7600, a breeding line with tomato accession Hawaii 7998 (H7998) in its pedigree. Race T4 resistance has also been described in the advanced breeding lines Fla. 8233, Fla. 8517, and Fla. 8326, and a selective genotyping approach was used to identify introgressions associated with resistance in segregating progeny derived from crosses with these lines. In the IBC population, loci on chromosomes 11 and 3, respectively, explained as much as 29.4 and 4.8% of resistance variation. Both these loci were also confirmed by selective genotyping: PI 114490 and H7998 alleles on chromosome 11 each provided resistance. The PI 114490 allele on chromosome 3 was confirmed in the Fla. 8517 population, and an allele of undetermined descent was confirmed at this locus in the Fla. 8326 population. A chromosome 12 allele was associated with susceptibility in the Fla. 8517 population. Additional loci contributing minor effects were also implicated in the IBC population or by selective genotyping. Selection for the major QTL in a marker-directed phenotyping approach should significantly improve the efficiency of breeding for resistance to bacterial spot race T4, although as yet undetected QTL would be necessary to carry out strict marker assisted selection.

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Authors and Affiliations

  1. Gulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 14625 CR 672, Wimauma, FL, 33598-6101, USA

    Samuel F. Hutton & Jay W. Scott

  2. Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan Xi Lu, Haidian District, Beijing, 100193, People’s Republic of China

    Wencai Yang

  3. Department of Horticulture and Crop Science, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH, 44691, USA

    Sung-Chur Sim & David M. Francis

  4. Plant Pathology Department, University of Florida, 2515 Fifield Hall, P.O. Box 110680, Gainesville, FL, 32611-0680, USA

    Jeffrey B. Jones

Authors
  1. Samuel F. Hutton
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  2. Jay W. Scott
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  3. Wencai Yang
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  4. Sung-Chur Sim
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  5. David M. Francis
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  6. Jeffrey B. Jones
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Correspondence to Samuel F. Hutton.

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Communicated by I. Paran.

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Hutton, S.F., Scott, J.W., Yang, W. et al. Identification of QTL associated with resistance to bacterial spot race T4 in tomato. Theor Appl Genet 121, 1275–1287 (2010). https://doi.org/10.1007/s00122-010-1387-5

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  • DOI: https://doi.org/10.1007/s00122-010-1387-5

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