Original Paper

Theoretical and Applied Genetics

, Volume 120, Issue 8, pp 1673-1687

Open Access This content is freely available online to anyone, anywhere at any time.

QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.)

  • N. BillotteAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement Email author 
  • , M. F. JourjonAffiliated withInstitut National de la Recherche Agronomique (INRA), Chemin de Borde-Rouge-Auzeville
  • , N. MarseillacAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
  • , A. BergerAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
  • , A. FloriAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
  • , H. AsmadyAffiliated withPT Socfin-Indonesia (SOCFINDO)
  • , B. AdonAffiliated withCentre National de Recherche Agronomique (CNRA)
  • , R. SinghAffiliated withMalaysian Palm Oil Board (MPOB)
  • , B. NouyAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
    • , F. PotierAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
    • , S. C. CheahAffiliated withMalaysian Palm Oil Board (MPOB)
    • , W. RohdeAffiliated withMax-Planck Institut für Züchtungsforschung (MPIZ)
    • , E. RitterAffiliated withCentro de Arkaute Departamento de Producción y Proteccion Vegetal (NEIKER)
    • , B. CourtoisAffiliated withCentre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement
    • , A. CharrierAffiliated withMontpellier SupAgro-University Montpellier II
    • , B. ManginAffiliated withInstitut National de la Recherche Agronomique (INRA), Chemin de Borde-Rouge-Auzeville

Abstract

A quantitative trait locus (QTL) analysis designed for a multi-parent population was carried out and tested in oil palm (Elaeis guineensis Jacq.), which is a diploid cross-fertilising perennial species. A new extension of the MCQTL package was especially designed for crosses between heterozygous parents. The algorithm, which is now available for any allogamous species, was used to perform and compare two types of QTL search for small size families, within-family analysis and across-family analysis, using data from a 2 × 2 complete factorial mating experiment involving four parents from three selected gene pools. A consensus genetic map of the factorial design was produced using 251 microsatellite loci, the locus of the Sh major gene controlling fruit shell presence, and an AFLP marker of that gene. A set of 76 QTLs involved in 24 quantitative phenotypic traits was identified. A comparison of the QTL detection results showed that the across-family analysis proved to be efficient due to the interconnected families, but the family size issue is just partially solved. The identification of QTL markers for small progeny numbers and for marker-assisted selection strategies is discussed.