Theoretical and Applied Genetics

, Volume 119, Issue 3, pp 497–505

Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun

  • Matthew N. Nelson
  • Annaliese S. Mason
  • Marie-Claire Castello
  • Linda Thomson
  • Guijun Yan
  • Wallace A. Cowling
Original Paper

DOI: 10.1007/s00122-009-1056-8

Cite this article as:
Nelson, M.N., Mason, A.S., Castello, MC. et al. Theor Appl Genet (2009) 119: 497. doi:10.1007/s00122-009-1056-8

Abstract

We analysed the products of male meiosis in microspore-derived progeny from a Brassica napus (AACnCn) × Brassica carinata (BBCcCc) interspecific hybrid (ABCnCc). Genotyping at 102 microsatellite marker loci and nuclear DNA contents provided strong evidence that 26 of the 28 progeny (93%) were derived from unreduced (2n) gametes. The high level of CnCc marker heterozygosity, and parallel spindles at Anaphase II in the ABCnCc hybrid, indicated that unreduced gametes were formed by first division restitution. The frequency of dyads at the tetrad stage of pollen development (2.6%) suggested that unreduced gametes were preferentially selected in microspore culture. Segregation of marker alleles in the microspore-derived progeny was consistent with homologous recombination between Cn and Cc chromosomes and homoeologous recombination involving A-, B- and C-genome chromosomes during meiosis in the ABCnCc hybrid. We discuss the potential for using microspore culture of unreduced gametes in interspecific hybrids to map Brassica centromeres through half-tetrad analysis.

Supplementary material

122_2009_1056_MOESM1_ESM.doc (27 kb)
Supplementary material 1 (DOC 27 kb)
122_2009_1056_MOESM2_ESM.doc (56 kb)
Supplementary material 2 (DOC 55 kb)
122_2009_1056_MOESM3_ESM.xls (62 kb)
Supplementary material 3 (XLS 61 kb)
122_2009_1056_MOESM4_ESM.doc (50 kb)
Supplementary material 4 (DOC 50 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Matthew N. Nelson
    • 1
  • Annaliese S. Mason
    • 1
  • Marie-Claire Castello
    • 1
  • Linda Thomson
    • 1
  • Guijun Yan
    • 1
  • Wallace A. Cowling
    • 1
  1. 1.Faculty of Natural and Agricultural Sciences, School of Plant BiologyThe University of Western AustraliaCrawleyAustralia

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