Theoretical and Applied Genetics

, Volume 117, Issue 7, pp 1181–1189

Contrasting population genetic structure and gene flow between Oryza rufipogon and Oryza nivara

Authors

  • Hai-Fei Zhou
    • State Key Laboratory of Systematic and Evolutionary BotanyInstitute of Botany, Chinese Academy of Sciences
  • Xiao-Ming Zheng
    • State Key Laboratory of Systematic and Evolutionary BotanyInstitute of Botany, Chinese Academy of Sciences
  • Ruo-Xun Wei
    • State Key Laboratory of Systematic and Evolutionary BotanyInstitute of Botany, Chinese Academy of Sciences
  • Gerard Second
    • The Research Institute for Development (IRD)
  • Duncan A. Vaughan
    • National Institute of Agrobiological Sciences
    • State Key Laboratory of Systematic and Evolutionary BotanyInstitute of Botany, Chinese Academy of Sciences
    • Graduate SchoolChinese Academy of Sciences
Original Paper

DOI: 10.1007/s00122-008-0855-7

Cite this article as:
Zhou, H., Zheng, X., Wei, R. et al. Theor Appl Genet (2008) 117: 1181. doi:10.1007/s00122-008-0855-7

Abstract

The cross compatible wild relatives of crops have furnished valuable genes for crop improvement. Understanding the genetics of these wild species may enhance their further use in breeding. In this study, sequence variation of the nuclear Lhs1 gene was used to investigate the population genetic structure and gene flow of Oryza rufipogon and O. nivara, two wild species most closely related to O. sativa. The two species diverge markedly in life history and mating system, with O. rufipogon being perennial and outcrossing and O. nivara being annual and predominantly inbreeding. Based on sequence data from 105 plants representing 11 wild populations covering the entire geographic range of these wild species, we detected significantly higher nucleotide variation in O. rufipogon than in O. nivara at both the population and species levels. At the population level the diversity in O. rufipogon (Hd = 0.712; θsil = 0.0017) is 2–3 folds higher than that in O. nivara (Hd = 0.306; θsil = 0.0005). AMOVA partitioning indicated that genetic differentiation among O. nivara populations (78.2%) was much higher than that among O. rufipogon populations (52.3%). The different level of genetic diversity and contrasting population genetic structure between O. rufipogon and O. nivara might be explained by their distinct life histories and mating systems. Our simulation using IM models demonstrated significant gene flow from O. nivara to O. rufipogon, indicating a directional introgression from the annual and selfing species into the perennial and outcrossing species. The ongoing introgression has played an important role in shaping current patterns of genetic diversity of these two wild species.

Supplementary material

122_2008_855_MOESM1_ESM.doc (39 kb)
Table S1 Populations of O. nivara and O. rufipogon sampled in the present study. (DOC 39 kb).

Copyright information

© Springer-Verlag 2008