Theoretical and Applied Genetics

, Volume 117, Issue 4, pp 499–511

A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses, based on 283 SSR and 501 SNP-based markers

Authors

    • IASMA Research Centre
  • Michela Troggio
    • IASMA Research Centre
  • Giuseppina Coppola
    • IASMA Research Centre
  • Angelica Jermakow
    • CSIRO Plant Industry
  • Dustin Cartwright
    • Myriad Genetics Inc.
  • Andrey Zharkikh
    • Myriad Genetics Inc.
  • Marco Stefanini
    • IASMA Research Centre
  • M. Stella Grando
    • IASMA Research Centre
  • Roberto Viola
    • IASMA Research Centre
  • Anne-Françoise Adam-Blondon
    • UMR 1165, INRA-CNRS-Université d Evry Génomique Végétale
  • Mark Thomas
    • CSIRO Plant Industry
  • Patrice This
    • UMR 1097, DIA-PC Equipe génétique Vigne, INRA Supagro
  • Riccardo Velasco
    • IASMA Research Centre
Original Paper

DOI: 10.1007/s00122-008-0794-3

Cite this article as:
Vezzulli, S., Troggio, M., Coppola, G. et al. Theor Appl Genet (2008) 117: 499. doi:10.1007/s00122-008-0794-3

Abstract

We have developed an integrated map from five elite cultivars of Vitis vinifera L.; Syrah, Pinot Noir, Grenache, Cabernet Sauvignon and Riesling which are parents of three segregating populations. A new source of markers, SNPs, identified in ESTs and unique BAC-end sequences was added to the available IGGP reference set of SSRs. The complete integrated map comprises 1,134 markers (350 AFLP®, 332 BESs, 169 ESTs, 283 SSRs) spanning 1,443 cM over 19 linkage groups and shows a mean distance between neighbouring loci of 1.27 cM. Marker order was mainly conserved between the integrated map and the highly dense Syrah × Pinot Noir consensus map except for few inversions. Moreover, the marker order has been validated through the assembled genome sequence of Pinot Noir. We have also assessed the transferability of SNP-based markers among five V. vinifera varieties, enabling marker validation across different genotypes. This integrated map can serve as a fundamental tool for molecular breeding in V. vinifera and related species and provide a basis for studies of genome organization and evolution in grapevines.

Supplementary material

122_2008_794_MOESM1_ESM.ppt (265 kb)
Supplementary Figure S1 (PPT 265 kb)
122_2008_794_MOESM2_ESM.ppt (1.3 mb)
Supplementary Figure S2 (PPT 1366 kb)
122_2008_794_MOESM3_ESM.xls (196 kb)
Table S1 (supplementary information). The 501 SNP-based markers mapped in the integrated map. (XLS 196 kb)
122_2008_794_MOESM4_ESM.xls (28 kb)
Table S2 (supplementary information). Distribution of marker type in the linkage groups and physical genome contigs. (XLS 28 kb)

Copyright information

© Springer-Verlag 2008